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Method for restoration of petroleum-polluted soil by aboriginal bacteria

A technology of oil pollution and indigenous bacteria, applied in the field of soil remediation, can solve the problems of high cost and heavy workload, and achieve the effect of avoiding death and enhancing ventilation

Inactive Publication Date: 2016-10-12
SOUTHWEST PETROLEUM UNIV
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AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of high cost and heavy workload in the remediation of oil-contaminated soil, and make full use of the degradation function of indigenous microorganisms in the soil, we provide a method for remediating oil-contaminated soil by using indigenous bacteria. The invention can achieve low cost , the purpose of reducing workload

Method used

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Embodiment

[0024] The specific implementation steps are as follows:

[0025] (1) Pretreatment of oil-contaminated soil: After removing the large debris in the oil-contaminated soil, air-dry it to a moisture content of about 20%, then use a grinder to grind the soil into small particles, and then divide the sample with 10 mesh The sieve sifts the soil.

[0026] (2) Screening and enrichment of indigenous bacteria: Weigh 2g of untreated petroleum-contaminated soil, add it to sterile water, shake at 35°C and 150rmp for 2h, inoculate 1mL of shaken soil suspension into 100mL of inorganic salt culture medium, and then cultured at 35°C, 150rmp for 3 days.

[0027] (3) Adding indigenous bacteria to the oil-contaminated soil: adding the screened and enriched indigenous bacteria to the oil-contaminated soil at a ratio of 40 mL / kg, so that the initial bacterial content of the oil-contaminated soil remediation reached 5.6×10 8 cells / g, and stirred.

[0028] (4) Dosing of inorganic nutrients and mo...

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Abstract

A method of using indigenous bacteria to restore oil-contaminated soil is to add mixed indigenous bacteria screened and enriched from oil-contaminated soil to oil-contaminated soil for bioremediation, and the specific steps include: oil-contaminated soil Grinding and sieving pretreatment, using inorganic salt medium to screen and enrich indigenous bacteria from oil-contaminated soil, adding enriched indigenous bacteria to oil-contaminated soil, and adding a certain proportion of inorganic nutrients to oil-contaminated soil Compost after stirring evenly, and plow and water the treated soil regularly. The method can be used for both in-situ remediation and ex-situ remediation of oil-contaminated soil, and can be used for remediation of high-concentration petroleum-contaminated soil and low-concentration petroleum-contaminated soil, and the medicines used are easy to obtain and easy to operate. And the degradation efficiency is high, and will not cause secondary pollution.

Description

technical field [0001] The invention belongs to the field of soil remediation, and in particular relates to a method for remediating petroleum-contaminated soil by using native bacteria. Background technique [0002] With the rapid development of the global industrialization process, the demand for petroleum products is also increasing. In the process of mining, production and transportation of petroleum and petroleum products, it is inevitable that a certain amount of petroleum and products will enter the soil, causing pollution. Petroleum is highly adhesive, which will affect some physical and chemical properties of the soil, and will also hinder the respiration of plant roots and the absorption of nutrients, seriously endangering the growth of plants. [0003] Although the soil has a certain self-purification ability for petroleum substances, when it exceeds this range, petroleum pollutants will accumulate in the soil, so that it will bring great harm to humans or the en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
CPCB09C1/105
Inventor 兰贵红杜娇严君华陈超邱海燕陈秀丽鲁莹纯李桂香
Owner SOUTHWEST PETROLEUM UNIV
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