Preparation method and application of petroleum-degrading bacteria immobilized embedded particles

A petroleum-degrading bacteria and petroleum-degrading technology, applied in the field of preparation of immobilized embedding particles of petroleum-degrading bacteria, can solve the problems of affecting the activity of the bacteria and the inability to guarantee the adsorption capacity of the carrier to the bacteria, so as to improve the permeability and increase the Microbial degradation ability and the effect of increasing the amount of bacteria absorbed

Active Publication Date: 2015-04-29
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method directly embeds the bacteria, which affects the activity of the bacteria, and cannot guarantee the amount of adsorption of the carrier to the bacteria. The prepared fly a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Preparation of auxiliary carrier

[0023] The water hyacinth and wheat straw are sun-dried, crushed, passed through an 80-mesh sieve, and then mixed with walnut shell activated carbon, of which water hyacinth is 46.5 wt%, wheat straw is 18.5 wt%, and walnut shell activated carbon is 35 wt%. The mixture was soaked in distilled water for 24 hours, adjusted to pH 7.0, filtered off the floating impurities and supernatant, and the filtered mixture was dried at 50°C for 24 hours and autoclaved at 121°C for 20 minutes, ready to be used as an auxiliary carrier.

[0024] The total carbon content of the water hyacinth in the auxiliary carrier is 250g / kg, the total nitrogen content is 43.8g / kg, and the total phosphorus content is 10.2g / kg. The total carbon content of wheat straw is 32.6 g / kg, total nitrogen content is 40.8 g / kg, and total phosphorus content is 0.76 g / kg. Walnut shell activated carbon is powdered activated carbon with a specific surface area of ​​960m 2 / g, the iodi...

Embodiment 2

[0035] One of the differences from Example 1 is that in the pretreatment process of the auxiliary carrier, the ratio of each material is: water hyacinth 43wt%, wheat straw 17wt%, walnut shell activated carbon 40wt%. The second difference is that when preparing the seed liquid of petroleum degrading bacteria, ensure that the number of petroleum degrading bacteria in the seed liquid reaches 3×10 10 Pieces / mL. The third difference is that when preparing immobilized embedded particles of petroleum degrading bacteria, the mass of polyvinyl alcohol added is 10wt%, sodium alginate 1.5wt%, and CaCl 2 The concentration of the solution is 3wt% in 10wt% Na 2 SO 4 The reaction time in the solution is 2h.

[0036] Other conditions are the same. Under the condition of 40 days of repair time, the removal rate of petroleum pollutants can reach 64.7%, and the removal rate of saturated hydrocarbons can reach 73%.

Embodiment 3

[0038] One of the differences from Example 1 is that in the pretreatment process of the auxiliary carrier, the ratio of each material is: water hyacinth 35.8 wt%, wheat straw 14.2 wt%, and walnut shell activated carbon 50 wt%. The second difference is that when preparing the seed liquid of petroleum degrading bacteria, ensure that the number of petroleum degrading bacteria in the seed liquid reaches 5×10 10 Pieces / mL. The third difference is that when preparing the immobilized embedded particles of petroleum degrading bacteria, the auxiliary carrier added is 3wt%, and cultured at 120r / min and 30℃ for 24h. The mass of PVA added is 8wt%, sodium alginate 1.2wt%, CaCl 2 The concentration of the solution is 5wt%, at 10wt% Na 2 SO 4 The reaction time in the solution is 2h. The fourth difference is that the oily soil contains 50g / kg of oil, and the amount of immobilized embedded particles of petroleum degrading bacteria is 1kg / m. 2 .

[0039] Other conditions are the same. Under the co...

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PUM

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Abstract

The invention relates to a preparation method of petroleum-degrading bacteria immobilized embedded particles. The preparation method comprises the following steps: (1) mixing 35.8wt%-46.5wt% of water hyacinth, 14.2wt%-18.5wt% of wheat straws and 35wt%-50wt% of walnut shell-based activated carbon to obtain a mixture as an auxiliary carrier; (2) inoculating 3.0wt%-7.0wt% of the auxiliary carrier onto 10wt%-20wt% of a petroleum-degrading bacteria seed liquid and adsorbing; (3) dissolving 8.0wt%-10wt% of polyvinyl alcohol in 80-100 DEG C water, adding 1.2wt%-1.5wt% of sodium alginate, uniformly mixing, cooling and adding the balance of water; and (4) uniformly mixing the substances obtained in the step (2) and (3) to constitute an embedded system, dropwise adding the embedded system into a CaCl2 solution and crosslinking to form balls, transferring the balls into a Na2SO4 solution and immobilizing to obtain the immobilized embedded particles. The petroleum-degrading bacteria immobilized embedded particles are applied in in-situ remediation of petroleum-polluted soil to achieve good remediation effect on the petroleum-polluted soil.

Description

Technical field [0001] The invention relates to the field of microbial immobilization, in particular to a preparation method and application of immobilized embedded particles of petroleum degrading bacteria for repairing petroleum contaminated soil. Background technique [0002] Petroleum contaminated soil is one of the important pollutions of petroleum and petrochemical companies. Petroleum pollutants are highly toxic to humans and the environment. People are paying more and more attention to soil pollution. It is becoming more and more important to ensure the efficiency of soil restoration and restore healthy production. [0003] The treatment methods of oil-contaminated soil at home and abroad mainly include incineration, landfill, stacking, and bioremediation. However, the biological method is the most economical and thorough, and it is the most effective means to achieve soil ecological restoration. In order to solve the shortcomings of free microorganisms and indigenous bac...

Claims

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Application Information

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IPC IPC(8): C12N11/14B09C1/10
Inventor 秦丽娇张胜中邓德刚周彤
Owner CHINA PETROLEUM & CHEM CORP
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