Phycomycete mixed microorganism preparation, preparation method and application thereof

A technology of mixing microorganisms and preparations, which is applied in the field of algae and bacteria mixed microbial preparations, can solve problems such as insufficient oxygen supply, achieve the effects of reducing repair costs, completely degrading, and solving the problem of dissolved oxygen limitation

Inactive Publication Date: 2009-10-21
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of the prior art, and to provide a mixed microbial preparation of algae and bacteria and its preparation method and application. The present invention aims at the problems faced by the above-men

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  • Phycomycete mixed microorganism preparation, preparation method and application thereof
  • Phycomycete mixed microorganism preparation, preparation method and application thereof

Examples

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Example Embodiment

[0036] Example 1: Algae and bacteria combination on crude oil degradation, optimization of the best bacteria and algae combination

[0037] The degradation effect of each combination of bacteria and algae on crude oil was determined to obtain the mixed preparation with the best degradation effect. The degradation rate of each component of crude oil is shown in the figure Figure 1 to Figure 4 .

[0038] (1) Petroleum component degrading bacteria degrade crude oil

[0039] GS3C is a petroleum component degrading bacteria derived from n-hexadecane as a carbon source. It has a very good degradation effect on the alkane component of crude oil, but does not degrade aromatic hydrocarbons; GY2B is a high-efficiency phenanthrene degrading strain, which is effective in degrading petroleum components. The naphthalene series, fluorene series and phenanthrene series of polycyclic aromatic hydrocarbons have a certain degradation effect, but can not use alkanes; GP3 is a mixture of two strains ...

Example Embodiment

[0048] Example 2: Biological polymorphism analysis of the mixed system of algae and bacteria

[0049] Use PCR-DGGE molecular biology method to analyze the polymorphism of each mixed system, and get the DGGE picture like Figure 5 Shown.

[0050] (1) Single species of algae

[0051]Figure 5 In the DGGE diagram of the single species of Scenedesmus system, there are 5 bands in the single species of algae system. The comparison results of their nucleic acid sequences in the GenBank database are shown in Table 1: Band 1 is the 16SrDNA fragment of Scenedesmus GH2, and the other 4 bands The belt corresponds to the four dominant bacteria. Band 2 has the highest similarity with Bacteroidetes bacteria; Band 3 is Hydrogenophaga; Band 4 is the closest to non-cultivable beta-proteobacterium; Band 5 is forearmophilus Methylophilus.

[0052] In the absence of algae growth, bands 2 and 4 also disappeared, indicating that these two bacteria must be attached to Scenedesmus in order to grow in the ...

Example Embodiment

[0061] Example 3: Preparation of a mixture of algae and bacteria and GC / MS analysis of its effect on crude oil degradation

[0062] The preparation of the mixed microbial preparation of algae and bacteria of the present invention: A single species of Scenedesmus is obtained by a plate separation method, and a lysozyme buffer is prepared, wherein the concentration of Tween80 in the lysozyme buffer is 0.005mol / l, EDTA accounts for 0.1mol / l, and SDS It accounts for 0.25mol / l, and lysozyme accounts for 0.5mg / ml. After adding lysozyme buffer to Scenedesmus to immerse Scenedesmus for 10 minutes, centrifuge to discard the supernatant, add sterile distilled water and repeat washing three times; add penicillin and gentamicin to the culture medium to sterilize Scenedesmus, get no Bacteria pure Scenedesmus; Pure Scenedesmus and total petroleum component degrading bacteria are inoculated with the culture medium at a volume ratio of 2:1, and the total petroleum component degrading bacteria are...

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Abstract

The invention discloses a phycomycete mixed microorganism preparation, a preparation method and the application thereof. The microorganism preparation provided by the invention is prepared by mixing sterile pure scenedesmus, alkane degrading bacteria GS3C, phenanthrene-degrading bacterial GY2B and pyrene degrading bacteria GP3. The combination of the alkane degrading bacteria and aromatic hydrocarbon degrading bacteria plays an excellent role in complementary degradation for alkane and aromatic hydrocarbon substance which are the main components in petroleum contamination. Algae can provide O2 needed by metabolizing crude oil for bacteria, and the simultaneously generated CO2 is utilized by the algae for growing. The mixed preparation can efficiently degrade and restore the petroleum contamination. Under the condition of crude oil contamination with the initial volume concentration of 0.3%, linear chain and branched paraffin which is difficult to degrade in alkane can be more thoroughly removed within 7 days, and the removal rates for naphthalin, fluorine, phenanthrene and homologous compound in aromatic hydrocarbon respectively reach over 96%, 58% and 61%.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, and in particular relates to a mixed microbial preparation of algae and bacteria as well as its preparation method and application. Background technique [0002] As a very important energy source, petroleum is widely used in the world, and the pollution caused by it is becoming more and more serious. According to incomplete statistics, oil and oil products leaked into water bodies through various channels account for about 0.5% of the world's total oil production every year, about 2 to 10 million tons. The oil content of the water quality in my country's coastal waters exceeds the national regulations by 2 to 8 times, and the pollution situation is very serious. [0003] Petroleum is a complex multi-component mixture, the main components of which are hydrocarbons (alkanes and aromatics, etc.). Among them, PAHs (polycyclic aromatic hydrocarbons) a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/12C02F3/34C12R1/89C12R1/38C12R1/01C02F101/32
Inventor 党志唐霞何丽媛易筱筠
Owner SOUTH CHINA UNIV OF TECH
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