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Phycomycete mixed microorganism preparation, preparation method and application thereof

A technology of mixing microorganisms and preparations, which is applied in the field of algae and bacteria mixed microbial preparations, can solve problems such as insufficient oxygen supply, achieve the effects of reducing repair costs, completely degrading, and solving the problem of dissolved oxygen limitation

Inactive Publication Date: 2009-10-21
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of the prior art, and to provide a mixed microbial preparation of algae and bacteria and its preparation method and application. The present invention aims at the problems faced by the above-mentioned existing water body oil pollution bioremediation technology, and designs complementary and synergistic degradation mechanisms. A good mixed bacterial agent, while using algae to provide O 2 , to solve the problem of insufficient oxygen supply in polluted waters

Method used

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  • Phycomycete mixed microorganism preparation, preparation method and application thereof
  • Phycomycete mixed microorganism preparation, preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0036] Example 1: The effect of the combination of algae and bacteria on crude oil degradation, the optimization of the best combination of bacteria and algae

[0037] The crude oil degradation effect of each combination of bacteria and algae was tested to obtain the mixed preparation with the best degradation effect. The degradation rate of each component of crude oil is shown in the figure Figure 1 to Figure 4 .

[0038] (1) Degradation of crude oil by petroleum component degrading bacteria

[0039] GS3C is a petroleum component-degrading bacterium obtained from n-hexadecane as a carbon source. It has a very good degradation effect on the alkane components of crude oil, but does not degrade aromatics; GY2B is a highly efficient phenanthrene-degrading strain. Naphthalene series, fluorene series, and phenanthrene series of polycyclic aromatic hydrocarbons have a certain degradation effect, but they cannot use alkanes; GP3 is a mixture of two strains of bacteria GP3A and GP3...

Embodiment 2

[0048] Example 2: Analysis of biological polymorphism in algae-bacteria mixed system

[0049] The polymorphism of each mixed system was analyzed by means of PCR-DGGE molecular biology, and the DGGE pictures were obtained as follows: Figure 5 shown.

[0050] (1) Single species of algae

[0051] Figure 5 In the DGGE map of the single Scenedesmus system, there are 5 bands in the single species of Scenedesmus, and the comparison results of their nucleic acid sequences in the GenBank database are shown in Table 1: Band 1 is the 16SrDNA fragment of Scenedesmus GH2, and the other 4 bands are The bands correspond to the four dominant bacteria. Band 2 has the highest similarity to Bacteroidetes; Band 3 is Hydrogenophaga; Band 4 is closest to unculturable β-proteobacterium; Band 5 is Mephiophilic Methylophilus.

[0052] In the case of no algae growth, bands 2 and 4 also disappeared, indicating that these two bacteria must attach to Scenedesmus to grow in the culture system.

[005...

Embodiment 3

[0061] Example 3: Preparation of algae-bacteria mixed preparation and GC / MS analysis of its effect on crude oil degradation

[0062] Preparation of algae-bacteria mixed microbial preparation of the present invention: utilize plate separation method to obtain single Scenedesmus, prepare lysozyme buffer solution, wherein the concentration of Tween80 in lysozyme buffer solution is 0.005mol / l, EDTA accounts for 0.1mol / l, SDS Accounted for 0.25mol / l, lysozyme accounted for 0.5mg / ml. Add lysozyme buffer solution in Scenedesmus and submerge Scenedesmus and place it for 10 minutes, centrifuge and discard the supernatant, add sterilized distilled water and repeat washing three times; add penicillin and gentamicin to the culture medium to sterilize Scenedesmus, and obtain Bacteria pure Scenedesmus; pure Scenedesmus and total petroleum component degrading bacteria were inoculated in the medium according to the volume ratio of 2:1, and the total petroleum component degrading bacteria were...

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Abstract

The invention discloses a phycomycete mixed microorganism preparation, a preparation method and the application thereof. The microorganism preparation provided by the invention is prepared by mixing sterile pure scenedesmus, alkane degrading bacteria GS3C, phenanthrene-degrading bacterial GY2B and pyrene degrading bacteria GP3. The combination of the alkane degrading bacteria and aromatic hydrocarbon degrading bacteria plays an excellent role in complementary degradation for alkane and aromatic hydrocarbon substance which are the main components in petroleum contamination. Algae can provide O2 needed by metabolizing crude oil for bacteria, and the simultaneously generated CO2 is utilized by the algae for growing. The mixed preparation can efficiently degrade and restore the petroleum contamination. Under the condition of crude oil contamination with the initial volume concentration of 0.3%, linear chain and branched paraffin which is difficult to degrade in alkane can be more thoroughly removed within 7 days, and the removal rates for naphthalin, fluorine, phenanthrene and homologous compound in aromatic hydrocarbon respectively reach over 96%, 58% and 61%.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, and in particular relates to a mixed microbial preparation of algae and bacteria as well as its preparation method and application. Background technique [0002] As a very important energy source, petroleum is widely used in the world, and the pollution caused by it is becoming more and more serious. According to incomplete statistics, oil and oil products leaked into water bodies through various channels account for about 0.5% of the world's total oil production every year, about 2 to 10 million tons. The oil content of the water quality in my country's coastal waters exceeds the national regulations by 2 to 8 times, and the pollution situation is very serious. [0003] Petroleum is a complex multi-component mixture, the main components of which are hydrocarbons (alkanes and aromatics, etc.). Among them, PAHs (polycyclic aromatic hydrocarbons) a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/12C02F3/34C12R1/89C12R1/38C12R1/01C02F101/32
Inventor 党志唐霞何丽媛易筱筠
Owner SOUTH CHINA UNIV OF TECH
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