Low-temperature-resistant petroleum degradation strains as well as culture method, culture medium and application thereof

Abstract

The invention relates to low-temperature-resistant petroleum degradation strains as well as a culture method, a culture medium and application thereof, wherein the low-temperature-resistant petroleum degradation strains are Paracoccus sp. D17 and Paracoccus sp. D24;a liquid culture medium for the two strains is screened, and per 1000ml of the liquid culture medium comprises 0.5g of NaCl, 0.1g of K2HPO4, 0.1g of NH4H2PO4, 0.1g of (NH4)2SO4, 0.02g of MgSO4.7H2O, 0.3g of KNO3, 700-900ml of oilfield wastewater and the balance of ultrapure water; a solid culture medium for the two strains is screened and is formed by additionally adding 15-20g of agar into each 1000ml of the liquid culture medium and further uniformly coating 20-30 mu l of sterile crude oil on the surface of the culture medium; and the two strains can be applied in degradation of petroleum hydrocarbon and bioremediation of petroleum pollution in soil or a water body. The two low-temperature strains can be applied in a low-temperature environment and have great value for controlling the petroleum pollution in the soil in the low-temperature environment.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to bacterial strains for degrading petroleum hydrocarbons in a low-temperature environment, liquid medium and solid medium for screening thereof, and their use in biological treatment of soil or water body oil pollution and environmental pollution restoration Applications. Background technique [0002] Petroleum is a viscous, flammable, yellow to black mixture of gaseous, liquid and solid hydrocarbons that occurs naturally beneath the surface of the earth and contains a variety of hydrocarbons (normal alkanes, branched alkanes, aromatic A complex mixture of cyclic hydrocarbons) and a small amount of other organic compounds (sulfides, nitrides, naphthenic acids, etc.). As an energy source, petroleum has become one of the most important sources of energy for all human beings. With the development of the economy, the human demand f...

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Problems solved by technology

Research on the microbial degradation of petroleum and its products is often reported, however, most of the previous research focused on the use of room temperature or high temperature microorganisms for environmental governance, and there are few corresponding studies on low temperature environments

In high mountains and high latitude areas, especially in the oil field areas in northern my country, the survival rate of microorganisms is low and the natural degradation ability is poor under cold climate conditions, which seriously affects the soil quality in oil-polluted areas.

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