Pseudomonas sp. LD23 and preparation of immobilized microspheres of Pseudomonas sp. LD23

A technology of Pseudomonas and microspheres, applied in the field of microorganisms, can solve problems such as lack of research

Active Publication Date: 2016-08-17
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few studies on the immobilization of petroleum-degrading bacteria using biochar as a carrier.

Method used

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  • Pseudomonas sp. LD23 and preparation of immobilized microspheres of Pseudomonas sp. LD23
  • Pseudomonas sp. LD23 and preparation of immobilized microspheres of Pseudomonas sp. LD23
  • Pseudomonas sp. LD23 and preparation of immobilized microspheres of Pseudomonas sp. LD23

Examples

Experimental program
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Effect test

Embodiment 1

[0028] This example illustrates strain selection.

[0029] The soil from the representative oil-polluted plots in Daqing was randomly mixed and sieved, and the mixed soil sample was weighed and enriched in the inorganic salt liquid wax medium for enrichment culture; the bacterial solution was drawn and connected to LB solid medium for gradient dilution Cloth, cultivated in a constant temperature incubator at 15°C for 3-5 days. A single colony with good growth was picked for strain purification, and a total of 13 strains of low-temperature bacteria were screened.

[0030] 1 Determination of bacterial growth curve

[0031] Select four representative strains of low-temperature bacteria (LD23, DDX82, DD4 and DD6) with good growth potential, inoculate them in LB liquid medium with 10% inoculum, cultivate them at 15°C, and inoculate them every 12 hours. Bacteria and non-inoculated medium to determine its OD 600 value to draw a growth curve. Such as figure 1 As shown, the select...

Embodiment 2

[0049] This example illustrates the preparation of sodium alginate-activated carbon immobilized microspheres.

[0050] Prepare 45mL of sodium alginate (SA) solution with a certain concentration, add appropriate amount of activated carbon, mix well, and sterilize at 121°C for 25min. Squeeze 5% CaCl into the sterile syringe 2 Formed in solution, cross-linked in a refrigerator at 4°C for a certain period of time, rinsed with normal saline or sterile water for 3 times, and stored in a refrigerator at 4°C to measure physical properties and oil degradation rate (see the table for the proportion of orthogonal experiments for each factor) 1). The prepared immobilized microspheres were added to the petroleum fermentation medium in an amount of 10%, cultured in a shaker at 15°C at 160r / min for 7 days, and then the degradation rate was measured by ultraviolet spectrophotometry, and the method was the same as above.

[0051] By measuring the degradation rate of LD23 petroleum-degrading ...

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Abstract

The invention relates to Pseudomonas sp. LD23, and the strain preservation number is CGMCC No,11679. The invention further relates to application of the Pseudomonas sp. LD23 in petroleum degradation and preparation of sodium alginate-activated carbon immobilized microspheres containing the Pseudomonas sp. LD23. The Pseudomonas sp. LD23 is a strain separated and screened from soil which is serious in petroleum pollution, can keep the high petroleum degradation rate under the low temperature condition and lays a foundation for petroleum-polluted soil treatment in future; meanwhile, the biological carbon immobilization conditions are optimized, the degrading effect of the immobilized petroleum degrading bacterium to raw petroleum is initially inspected, and a scientific basis is provided for remedying petroleum oil through the biological carbon immobilized microspheres.

Description

technical field [0001] The invention belongs to the field of microorganisms and relates to the preparation of a strain of pseudomonas LD23 and its immobilized microspheres. Background technique [0002] As the global industry enters a period of rapid development, the demand for petroleum and its products is increasing day by day. Due to a large number of exploitation and use, as well as the bursting of some underground oil pipelines and the occurrence of leakage accidents of underground oil storage tanks, a large amount of oil and its processed products have entered the soil, which has brought harm to living things and human beings. Oil entering the soil will affect soil permeability, reduce soil fertility, and hinder plant growth, and the pollutants in petroleum hydrocarbons that are not easily absorbed by the soil can penetrate into groundwater with surface precipitation, directly affecting the quality of drinking water; PAHs are carcinogenic, teratogenic, and mutagenic, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/14B09C1/10A62D3/02C12R1/38A62D101/20
CPCA62D3/02A62D2101/20B09C1/10C12N11/10C12N11/14C12N1/205C12R2001/38
Inventor 曹迪苑泽宁丛明阳魏海东陈梦晨白琳刘安娜
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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