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Microbial remediation curing adsorbing bacterial preparation applicable to marine environment petroleum pollution as well as preparation method and application of same

A technology for microbial remediation and oil pollution, applied in the field of microbial biotechnology and environmental biology, can solve the problems of low density of inoculants, limited application scope of inoculants, and cannot be used in areas where seabed sediments cannot be used, so as to prolong residence time and improve survival. rate, the effect of expanding the scope of application

Active Publication Date: 2013-04-17
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem that the repairing bacteria used in the existing microbial repairing method has a low density of the bacteria and cannot be applied to the repairing area of ​​seabed sediments, etc., thus limiting the application range of the bacteria, and provides a method suitable for use in the ocean. Microbial remediation of oil pollution in marine environments such as tidal flats and seabed sediments Preparation method of solidified adsorption bacteria agent, and its application in bioremediation of marine environmental oil pollution such as marine tidal flats and seabed sediments

Method used

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  • Microbial remediation curing adsorbing bacterial preparation applicable to marine environment petroleum pollution as well as preparation method and application of same
  • Microbial remediation curing adsorbing bacterial preparation applicable to marine environment petroleum pollution as well as preparation method and application of same
  • Microbial remediation curing adsorbing bacterial preparation applicable to marine environment petroleum pollution as well as preparation method and application of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, the preparation method of Rhodococcus T7-3 solidified adsorption bacterial agent

[0032] 1. Screening of degrading bacteria

[0033] Collect crude oil from polluted areas (this example temporarily takes the mixed crude oil of Haier Station as an example, hereinafter referred to as crude oil), and add it to inorganic salt medium (KH2 PO 4 3.48g / L, Na 2 HPO 4 1.5g / L, MgSO 4 0.70g / L, (NH 4 ) 2 SO 4 4g / L, NaCl 20g / L, yeast powder 0.05g / L, pH 7.0~7.2) to form a crude oil inorganic salt medium, sterilized at 121°C for 20min, and screened strains with good crude oil degradation performance, the culture condition was 25 ℃, 150r / min, inoculum size 2%, culture time 7d.

[0034] In this example, Rhodococcus erythoropolis T7-3 was selected from 30 strains to be selected, (it was preserved in the "General Microbiology Center of China Committee for the Collection of Microbial Cultures" on May 11, 2012, and its address is Beijing Institute of Microbiology, C...

Embodiment 2

[0055] Embodiment 2, the preparation method of Burkholderia ATCC23344 solidified adsorption bacterial agent

[0056] 1. Screening of degrading bacteria

[0057] Collect crude oil from polluted areas (this example temporarily takes the mixed crude oil of Haier Station as an example, hereinafter referred to as crude oil), and add it to inorganic salt medium (KH 2 PO 4 3.48g / L, Na 2 HPO 4 1.5g / L, MgSO 4 0.70g / L, (NH 4 ) 2 SO 4 4g / L, NaCl 20g / L, yeast powder 0.05g / L, pH 7.0~7.2), to screen strains with good oil degradation performance, culture conditions are 25℃, 150r / min, inoculum size 2%, culture time for 7d.

[0058] In this embodiment, Burkholderia mallei ATCC 23344 (Burkholderia mallei ATCC 23344, purchased from the American Type Culture Center, its item number is ATCC23344, hereinafter referred to as ATCC23344) was selected from 30 strains to be selected, and inoculated into uninoculated crude oil inorganic Salt culture medium, the crude oil degradation rate of ...

Embodiment 3

[0067] Embodiment 3, the preparation method of Acinetobacter ATCC19194 solidified adsorption bacteria agent

[0068] 1. Screening of degrading bacteria

[0069] Collect crude oil from polluted areas (this example temporarily takes the mixed crude oil of Haier Station as an example, hereinafter referred to as crude oil), and add it to inorganic salt medium (KH 2 PO 4 3.48g / L, Na 2HPO 4 1.5g / L, MgSO 4 0.70g / L, (NH 4 ) 2 SO 4 4g / L, NaCl 20g / L, yeast powder 0.05g / L, pH 7.0~7.2), to screen strains with good oil degradation performance, culture conditions are 25℃, 150r / min, inoculum size 2%, culture time for 7d.

[0070] In this example, Acinetobacter haemolyticus ATCC 19194 (Acinetobacter haemolyticus ATCC 19194, purchased from the American Type Culture Center, its article number is ATCC19194, hereinafter referred to as ATCC 19194) was selected from 30 strains to be selected, and inoculated to cultured with uninoculated crude oil inorganic salts. Based on the crude oil...

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Abstract

The invention provides a microbial remediation curing adsorbing bacterial preparation applicable to marine environment petroleum pollution as well as a preparation method and an application of the microbial remediation curing adsorbing bacterial preparation. The preparation process of the curing adsorbing bacterial preparation comprises the following steps: selecting microbial strains suitable for degrading crude oil in a polluted area; enlarging cultivation step by step so as to obtain a fermenting bacteria liquid with bacteria concentration of 108-1010 bacteria / mL; and then adding 100-1000 g / L porous medium (zeolite or ceramsite) to the fermenting bacteria liquid; further adding 0.1-2 g / L flocculating agent to the fermenting bacteria liquid; sufficiently mixing the fermenting bacteria liquid, the porous medium and the flocculating agent; standing an obtained mixture for 0.5-24 h so as to prepare the curing adsorbing bacterial preparation with bacterial adsorbing rate of not less than 90%. The curing adsorbing bacterial preparation can be applied to biological remediations of marine environment petroleum pollutions such as marine shoal muds or marine bottom sediments; highly efficient petroleum hydrocarbon degrading bacteria and nutritions can be carried in the marine bottom sediments; and the retention times of the highly efficient petroleum hydrocarbon degrading bacteria and the nutritions in the shoals can be prolonged; the survival rate of target bacteria in a target location is obviously increased; and the application range of a remediation bacterial preparation is enlarged.

Description

technical field [0001] The invention belongs to the field of microbial biotechnology and environmental biotechnology, and specifically relates to a method for preparing a microbial solidification and adsorption bacterial agent suitable for the restoration of oil pollution in marine tidal flats and seabed sediments, and its application in marine tidal flats and seabed sediments to oil pollution. applications in bioremediation. Background technique [0002] With the frequent occurrence of offshore oil development and tanker accidents, offshore oil pollution is becoming more and more serious, which directly affects the development of marine aquaculture and fishing industries. Oil spills at sea not only directly cause pollution hazards, but also cannot be completely removed, some residual oil is difficult to degrade under natural conditions, and toxic and harmful components are accumulated, thus causing long-term damage to marine ecology. According to the national monitoring re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14A62D3/02C12R1/07C12R1/38C12R1/01A62D101/20
Inventor 马挺李国强梁凤来
Owner NANKAI UNIV
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