Bacillus cereus and application thereof

A technology of Bacillus cereus and preservation number, applied in the field of bioengineering, can solve the problems of long duration and slow degradation rate of petroleum hydrocarbon pollutants, and achieve the effects of improving physical properties, optimizing soil microbial system, and enhancing biological activity

Inactive Publication Date: 2013-10-16
CHANGAN UNIV
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  • Abstract
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Problems solved by technology

[0002] The microbial remediation technology of oil-contaminated soil has been greatly developed in foreign countries in the past ten years, but the degradation rate of petroleum hydrocarbon pollutants is slow, resulting in a long duration of the treatment process, which has always been a prominent difficulty in this technology, so looking for High-efficiency petroleum-degrading bacteria are currently a research hotspot in microbial remediation of petroleum-contaminated soils

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  • Bacillus cereus and application thereof
  • Bacillus cereus and application thereof

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Embodiment Construction

[0028] Screening of strains:

[0029] Step 1. Enrichment: The soil samples collected from the soil oil pollution 10000mg / kg restoration area of ​​the red clover rhizosphere restoration area about 10cm away from the Shaanxi Provincial Groundwater and Ecological Environment Engineering Research Center Test Site in Weiyang District, Chang'an University, Weishui Campus, Xi'an City were sterile Weigh 10.0g, put it into 100.0mL petroleum / inorganic salt liquid medium, and place it in a constant temperature shaker at 28±1℃, 130r / min for enrichment culture for 7 days; In the inorganic salt liquid medium, enrichment and domestication were carried out 3 times, each time for 7 days; the composition of the petroleum / inorganic salt liquid medium was: NaNO 3 0.15%, (NH 4 ) SO 4 0.15%, K 2 HPO 4 0.1%, MgSO 4 ·7H 2 O 0.05%, KCl 0.05%, FeSO 4 ·7H 2 O 0.001%, CaCl 2 0.0002%, distilled water 1000mL, crude oil (Yanchang Petroleum Dehydrated Crude Oil) 0.5%, the above are mass percentage...

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Abstract

The invention discloses a bacillus cereus Bacillus cereus 4033. The conservation number of the bacillus cereus is CGMCCNO.6074. The invention also discloses the application of the bacillus cereus in degrading oil pollutant. The bacillus cereus is derived from soil, so that the strain rapidly becomes dominant strain after entering into the soil, occupies a certain ecological niche, effectively promotes degradation of oil hydrocarbon, optimizes the soil microbe system, improves the physical behavior of soil, enhances the biological activity of soil, and restores hardened and degraded soil. The bacillus cereus is a dominant bacteria which is selected by using crude oil as the sole carbon source, the property of crude oil can be changed in the process of growth and multiplication, components of crude oil can be changed, the functions of emulsifying, wetting and dispersing crude oil are realized, and the oil pollutant in the soil can be degraded in a short time.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a bacillus cereus and an application thereof. Background technique [0002] The microbial remediation technology of oil-contaminated soil has been greatly developed in foreign countries in the past ten years, but the degradation rate of petroleum hydrocarbon pollutants is slow, resulting in a long duration of the treatment process, which has always been a prominent difficulty in this technology, so looking for High-efficiency petroleum-degrading bacteria are currently a research hotspot in the microbial remediation technology of petroleum-contaminated soil. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a Bacillus cereus that can be used to degrade petroleum pollutants in view of the above-mentioned deficiencies in the prior art. This strain is a dominant strain selected from crude oil as the only...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10A62D3/02C12R1/085A62D101/20
Inventor 申圆圆王文科李春荣王周峰
Owner CHANGAN UNIV
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