Efficient repairing complex inoculant and application thereof

A composite bacterial agent and water restoration technology, which is applied in the biological field, can solve the problems of single microbial restoration technology, cure the symptoms but not the root cause, instability, etc., and achieve the effect of resource-based treatment, significant effect, and saving operating costs

Inactive Publication Date: 2014-01-01
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. Although a single artificial aeration and reoxygenation technology can effectively improve the water quality in a short period of time, it has disadvantages such as temporary, unstable, and temporary solutions, and the efficiency of nitrogen and phosphorus removal is low
[0007] 2. Single microbial remediation technology

Method used

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  • Efficient repairing complex inoculant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Enrichment medium:

[0026] ① Yeast: glucose 100g, peptone 7g, yeast extract 7g, KH2PO41g, MgSO40.5, distilled water 1L, pH 4.0-6.5;

[0027] ② Photosynthetic bacteria: NH4Cl0.1g, NaHCO 3 0.1g (5% aqueous solution, filter sterilized to take 2ml to add), K 2 HPO 4 0.02g, sodium acetate 0.3g, MgSO 4 7H 2 O0.02g, NaCl0.2g, growth factor 1ml, trace element solution 1ml, distilled water 97ml, pH7.0.

[0028] ③ Heterotrophic nitrifying bacteria: beef peptone 15g / L, NaCl 5g / L, pH7.0: 25-35℃.

[0029] Separation medium:

[0030] ①Yeast: Potatoes 200g (peeled, poached, washed, sliced, weighed 200g, put in 1000ml tap water, boiled for 30min, filtered with double gauze, and the filtrate was diluted with water to 1000ml), glucose 20g, lactic acid 5ml, agar 20g , distilled water 1000ml, pH 4.0-6.5;

[0031] ② Photosynthetic bacteria: NH4Cl0.1g, NaHCO 3 0.1g (5% aqueous solution, filter sterilized to take 2ml to add), K 2 HPO 4 ,0.02g,sodium acetate 0.3g,MgSO 4 7H 2 ...

Embodiment 2

[0045] 1. Zhuanhe (including Taiping Lake) and North Moat (Songlin Gate-Paper Mill Ship Lock) water bloom prevention and control projects

[0046] The COD, nitrogen, phosphorus, etc. in the Zhuanhe River and the North Moat water exceeded the standard, and the water bloom pollution was serious. In order to solve this type of pollution control, in 2012, the high-efficiency restoration compound bacterial agent expanded and cultivated in Example 1 was added to remove the excessive water in the water body. COD, nitrogen, phosphorus, etc. (COD, nitrogen, phosphorus, and transparency were 67mg / L, 31.2mg / L, 2.26mg / L, and 30cm before adding, and COD, nitrogen, phosphorus, and transparency were 23mg / L after adding L, 7.4mg / L, 0.18mg / L, 70cm), the water bloom has been effectively controlled.

[0047] 2. Beijing Nansha River standard treatment project

[0048] In 2011, the COD, nitrogen, and phosphorus in the Nansha River in Beijing exceeded the standard seriously, and the water bloom wa...

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Abstract

The invention relates to the field of biology and in particular to an efficient repairing complex inoculant and application thereof. The complex inoculant provided by the invention comprises strains of rhodococcus, pseudomonas, saccharomyces and micrococcus luteus, where the preservation number of the rhodococcus is CGMCC No.3276; the preservation number of the pseudomonas is CGMCC No.3277; the preservation number of the saccharomyces is CGMCC No.3278; the preservation number of the micrococcus luteus is CGMCC No.3903. The complex inoculant has remarkable effects of water ecosystem restoration, water bloom prevention and deodorization, in addition, the removal rate of the complex inoculant to all organic matters reach more than 70 percent, and national policies of circular economy and energy conservation and emission reduction are met better. By using the complex inoculant, a large amount of running cost for various water areas can be saved, and recycling treatment to wastewater is achieved.

Description

technical field [0001] The invention relates to the field of sub-biology, in particular to a high-efficiency repairing composite bacterial agent and its application. Background technique [0002] During the formation and development of the water biological chain, each period has its specific ecological characteristics and fixed structural composition, thus showing certain ecological processes such as material circulation and energy flow. When the external disturbance is weak, the water biological chain will show a certain self-regulation ability to maintain its inherent structure and function; when the interference exceeds its self-regulation ability, the structure and function of the biological chain will undergo greater variation. There are even broken links. The structure and function of the broken water biological chain generally manifests as the decline of species diversity, the loss of population regulation ability, the decline of primary productivity of the system, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/38C12R1/85C12R1/265C12R1/01
Inventor 李捍东
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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