High temperature aerobic fermentation bacteria and application of high temperature aerobic fermentation bacteria in quantitative reduction and resource recovery of domestic garbage, sludge and other solid waste

A technology of aerobic fermentation and domestic waste, which is applied in the field of microorganisms to achieve the effect of rapid reduction and treatment of waste

Inactive Publication Date: 2013-04-03
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been no reports about the successful application of high-temperature bacteria in waste reduction treatment in China.

Method used

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  • High temperature aerobic fermentation bacteria and application of high temperature aerobic fermentation bacteria in quantitative reduction and resource recovery of domestic garbage, sludge and other solid waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the screening of high-temperature aerobic fermentation bacteria

[0043] 1. Isolation of high-temperature aerobic fermentation bacterial strains

[0044] Collect mud samples from 8 points of high-temperature hot springs and 10 points of high-temperature pools of power plants, and enrich and cultivate them in liquid enrichment medium for 3-10 days. Continue culturing the enriched culture solution on the separation medium plate at a temperature of 75-80°C to obtain a single isolated strain. Then, the isolated single bacterial strain was purified, isolated and cultivated for 2-6 times, thereby obtaining 10 strains of high-temperature aerobic fermentation bacteria.

[0045] Among them, the formula of the enrichment medium is: beef peptone 15g / L, yeast extract 4g, NaCl 4g / L, pH 8.0, 75°C-80°C.

[0046] The formulation of the separation medium is: beef peptone 15g / L, yeast extract 4g, NaCL 4g / L, agar 20g / L, pH 8.0, 75°C-80°C.

[0047] 2. Preparation of high-t...

Embodiment 2

[0054] Embodiment 2, use the high-temperature aerobic fermentation bacteria of the present invention to process garbage

[0055] 1. Expanded cultivation of high-temperature aerobic fermentation bacterial compound bacterial agent

[0056] The three strains (M1, M2, and F1 combined according to the ratio of 1:1:2) screened in Example 1 for expanded culture were expanded and cultured in the required amount by using a compound bacterial culture medium.

[0057] The composition of the compound culture medium is: beef peptone 15g / L, yeast extract 4g, NaCL 4g / L, pH8.0, cultured at 75-80°C.

[0058] 2. Garbage degradation

[0059] 1) Start-up of the biodegradation system

[0060] Taking the waste treatment capacity as 300kg as an example, add 100kg of sawdust with a moisture content of 50%-60% prepared in advance into the processing equipment, and then add 1.5kg of the above-mentioned high-temperature aerobic fermentation bacterial compound agent and 1.5kg of porous perlite particle...

Embodiment 3

[0066] Embodiment 3, high-temperature aerobic fermentation bacterium of the present invention is used as the index determination of rubbish degrading bacterial agent

[0067] 1) Determination of genetic stability

[0068] The three high-temperature aerobic fermentation bacteria can remain stable genetically within 15 generations.

[0069] 2) Determination of antibiotic susceptibility

[0070] Three antibiotics sensitive to high-temperature aerobic fermentation bacteria are chloramphenicol, tetracycline, penicillin G, cephalothin (cephalosporin No. I), gentamicin, ofloxacin (ofloxacin), polymyxin B, gram Lindamycin (clindamycin), ketoconazole, miconazole / dactomazole, and clotrimazole.

[0071] 3) Determination of acute skin irritation to rabbits

[0072] The three high-temperature aerobic fermentation bacteria were slightly irritating to the acute skin irritation of rabbits.

[0073] 4) Determination of acute oral toxicity in mice

[0074] The acute oral toxicity test resu...

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Abstract

The present invention relates to the field of microorganism, specifically to high temperature aerobic fermentation bacteria, and an application of the high temperature aerobic fermentation bacteria in quantitative reduction and resource recovery of domestic garbage, sludge and other solid waste. The screened strains of the present invention comprise bacillus sp., pseudomonas sp. and thermus sp.. The preservation numbers of the strains are CGMCC NO. 4020, CGMCC NO. 4021 and CGMCC NO. 4022 respectively. According to the present invention, an in situ multi-point sampling method is adopted to screen plural strains of the microorganisms so as to overcome the difficulty of poor stability of the cultured bacteria solution by the single-point sampling method; a plurality of single factor experiments and orthogonal experiments are adopted to realize the optimal selection of the strains, and master the optimal growth conditions of the composite bacteria agent; with the successful application ofthe high temperature bacteria in the treatments of the garbage, the sludge and other solid waste, the technical bottleneck problems of the treatments of the garbage, the sludge and other solid waste are solved, the purposes of the quantitative reduction and the resource recovery of the garbage are achieved.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular, the invention relates to a high-temperature aerobic fermentation bacterium and its application in the reduction and recycling of solid waste such as domestic garbage and sludge. Background technique [0002] With the rapid development of social economy, the continuous improvement of people's living standards and the rapid growth of population, the corresponding amount of urban domestic waste is also increasing, and the contradiction between waste production and consumption is becoming more and more prominent. Up to 500 million square meters. The garbage dump is close to saturation, the processing capacity is seriously insufficient and does not meet the needs of standardized garbage disposal, and it pollutes the surrounding environment. New technologies for waste reduction, resource utilization and efficient disposal are urgently needed. [0003] At present, the industrialized treatme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/07C12R1/38C12R1/01
CPCY02A40/20Y02W30/40
Inventor 李捍东
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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