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Traditional Chinese medicine antibacteria composition for inhibiting micrococcus luteus

一种藤黄微球菌、组合物的技术，应用在中药领域，能够解决影响人体健康、一次用剂量大等问题，达到增强大黄抑菌性能、降低用量的效果

Active Publication Date: 2014-09-03
ANSON BIO-TECH(HUNAN) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Concentrated Chinese medicine preparations made into oral liquids or antibacterial agents, coating agents, etc. have a large one-time dosage, and long-term use will affect human health

Method used

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  • Traditional Chinese medicine antibacteria composition for inhibiting micrococcus luteus
  • Traditional Chinese medicine antibacteria composition for inhibiting micrococcus luteus
  • Traditional Chinese medicine antibacteria composition for inhibiting micrococcus luteus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. The preparation process of rhubarb extract:

[0025] Weigh 50 g of rhubarb, add 450 mL of water, extract at 95 °C for 4 h, pass through a vacuum rotary concentrator, use a 0.22 μm filter to remove impurities and bacteria in the extract, and finally use high performance liquid chromatography to measure The emodin content in the extract was 1.92 μg / g.

[0026] 2. Preparation of water-soluble silver nanoparticles

[0027] Take 22.8 g of myristic acid and dissolve it in 140 mL of methanol and water mixed at a ratio of 2:5, add 4 g of sodium hydroxide to the solution to precipitate a precipitate, filter, add the precipitate to 100 mL of 10 mol / L water-soluble Silver myristate was obtained in a neutral silver nitrate solution; 6.7 g of silver myristate with a concentration of 20 mmoL / L was weighed in a 100 mL beaker, and 58 mL of triethylamine with a concentration of 40 mmoL / L was added to the beaker , under electromagnetic stirring at 80°C for 2 h, the white silver myri...

Embodiment 2

[0043] 1. The preparation process of rhubarb extract:

[0044] With embodiment 1.

[0045] 2. Preparation of water-soluble silver nanoparticles

[0046]With embodiment 1.

[0047] 3. The synergistic antibacterial effect of rhubarb extract and water-soluble nano-silver.

[0048] a. Culture of bacteria

[0049] Pick a single colony of Escherichia coli O157:H7 on LB agar medium, place it in 10 mL LB broth medium, culture it in a 37 °C incubator for 16 h, and then inoculate it into 5 mL LB at an inoculum size of 1%. After incubation in broth medium for 4 h at 37 °C. Take 1 mL of the above bacterial solution and serially dilute three gradients with 0.1% peptone water, the final bacterial solution is about 10 6 CFU / mL.

[0050] b. The synergistic effect of water-soluble nano-silver and rhubarb extract on antibacterial properties

[0051] Take 1 mL of the above-mentioned diluted bacterial solution in 1.5 mL sterilized centrifuge tubes, add 150 μL of rhubarb extract to No. 1 c...

Embodiment 3

[0062] 1. The preparation process of rhubarb extract:

[0063] With embodiment 1.

[0064] 2. Preparation of water-soluble silver nanoparticles

[0065] With embodiment 1.

[0066] 3. The synergistic antibacterial effect of rhubarb extract and water-soluble nano-silver.

[0067] a. Culture of bacteria

[0068] Pick a single colony of Micrococcus luteus on the beef extract-peptone agar medium and put it in 5 mL of beef extract-peptone liquid medium, culture it in a 30 °C incubator for 24 h, and then inoculate it in 5 mL of After cultured in beef extract peptone liquid medium at 30 °C for 12 h. Take 1 mL of the above bacterial solution and serially dilute three gradients in a mixture of PBS and liquid medium at a ratio of 4:1, and the final bacterial solution is about 10 6 ~10 7 CFU / mL.

[0069] b. The synergistic effect of water-soluble nano-silver and rhubarb extract on antibacterial properties

[0070] Take 1 mL of the above-mentioned diluted bacterial solution into ...

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Abstract

The invention discloses a traditional Chinese medicine antibacteria composition for inhibiting micrococcus luteus, and belongs to the traditional Chinese medicine field. A rheum officinale extracting solution and water-soluble nano-silver are mixed in the antibacteria composition in proportion, two medicines are synthesized to play the synergistic effect to inhibit the micrococcus luteus. Compared with the prior art, the antibacteria composition containing the rheum officinale extract and the nano-silver can solve the problem that the dosage of the rheum officinale bacteriostatic agent in the conventional antibacteria method is great; and meanwhile, the microorganism medicine resistance generating rate is reduced by adopting two antibacterial substances in low dosage, the toxicity to the human body is lowered, and the composition is safer.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, in particular to a traditional Chinese medicine antibacterial composition. Background technique [0002] Rhubarb is a collective name for various perennial plants of the Polygonaceae Rhubarb genus, and it is also the name of Chinese medicinal materials. Because it likes a cool climate and is resistant to cold, it mainly grows in the damp environment on the edge of mountain forests or grass slopes. The traditional Chinese medicine rhubarb has the functions of attacking stagnation, clearing damp and heat, purging fire, cooling blood, removing blood stasis, and detoxifying. Modern pharmacological studies have proved that rhubarb has antibacterial effects, including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Shigella, hemolytic streptococcus, pneumococcus, diphtheria bacillus, Bacillus anthracis, Proteus, etc. Strong inhibitory effect, in addition,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/708A61P31/04A61K33/38
CPCY02A50/30
Inventor 许恒毅熊勇华王力均郭亮徐锋万翠香魏华赖卫华
Owner ANSON BIO-TECH(HUNAN) CO LTD
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