Preparation and application of Paenibacillus polymyxa gy40 and its wettable powder

The technology of polymyxa spore and wettable powder is applied to the fermentation and cultivation of Paenibacillus polymyxa GY40 strain, the preparation of bacterial powder, wettable powder, and the field of Paenibacillus polymyxa GY40 strain, which can solve the problem of less excellent strains in production. , poor adaptability, high cultivation temperature

Active Publication Date: 2021-04-06
JIANGSU GENGYUN CHEM CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is aimed at the deficiencies of the prior art, especially the deficiencies such as fewer excellent bacterial strains in production, poor adaptability, and higher cultivation temperature, and provides a salt-tolerant, low-cultivation temperature and simple nutrition derived from the ocean. A New Paenibacillus polymyxa GY40 Strain CGMCC NO.14481

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  • Preparation and application of Paenibacillus polymyxa gy40 and its wettable powder
  • Preparation and application of Paenibacillus polymyxa gy40 and its wettable powder
  • Preparation and application of Paenibacillus polymyxa gy40 and its wettable powder

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Embodiment 1

[0313] The specific technical solutions of the present invention are further described below, so that those skilled in the art can further understand the present invention, without limiting their rights. Example 1, a strain of Paenibacillus polymyxa GY40, its preservation number is: CGMCC NO.14481.

[0314] The spore-producing fermentation culture method of the described Paenibacillus polymyxa GY40 bacterial strain, its steps are as follows:

[0315] (1) Inoculate the GY40 strain on the PDA medium and activate it for 24 hours. Under sterile conditions, pick the GY40 strain with an inoculation loop and place it in a 250mL Erlenmeyer flask containing 60mL of seed liquid medium, and cultivate it at 28°C for 16h with shaking at 180r / min. ; Adjust the bacterial concentration to 10 9 cells / mL, as seed liquid for fermentation; the formula of the seed liquid culture medium is: glucose 20g / L, potato 200g / L, water 1L;

[0316](2) The seed solution is inoculated into a fermenter equipp...

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Abstract

The invention discloses a Paenibacillus polymyxa GY40 strain, and its preservation number is: CGMCC NO.14481. The invention also discloses a spore-forming fermentation culture method of the Paenibacillus polymyxa GY40 strain and a method for preparing bacteria powder and wettable powder by using the Paenibacillus polymyxa GY40 strain. The bacterial strain of the present invention has the advantages of strong salt tolerance, low growth temperature, and strong antibacterial effect that terrestrial microorganisms do not have; its spore-forming fermentation method has a simple formula, and the raw materials are mainly agricultural product leftovers, with low price, wide sources, and rapid growth of the bacterial strain. Fast, low fermentation temperature, short fermentation time, easy control of fermentation conditions, high yield of fermentation spores, reduced cost of raw materials, reduced energy consumption of fermentation, improved fermentation production efficiency, the wettable powder prepared by this strain has good control Wheat head blight and cucumber wilt can be used for wheat and cucumber planting.

Description

technical field [0001] The present invention relates to a bacterial strain derived from the ocean, in particular to a Paenibacillus polymyxa GY40 bacterial strain, and the present invention also relates to a fermentation and cultivation method of the Paenibacillus polymyxa GY40 bacterial strain, using the bacterial strain to prepare bacteria powder and wettable powder methods and uses. Background technique [0002] Bacillus can produce endophytic spores with strong stress resistance, and the spores are suitable for storage and transportation, so they are the main active ingredients of the developed microbial agents. At present, most of the successfully developed biocontrol live bacteria preparations use spores as the main component. To study the spore-producing medium and fermentation conditions of the strains, and to improve the fermentation level and spore yield is the key to the development of live bacillus preparations, which is of great significance for improving produc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01N25/14A01P3/00C12R1/12
CPCA01N25/14A01N63/00A01N63/10C12N1/20C12N1/205C12R2001/12
Inventor 李楠贾杰张海军刘慕兰李惠陶忠
Owner JIANGSU GENGYUN CHEM CO LTD
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