Fermentation production method and culture medium of hypocrea virens

A technology for bacillus and production method, applied in the field of fermentation production method and culture medium of Paenibacillus crebens, can solve the problems of inability to meet the application of preventing plant diseases, inability to meet plant diseases, and high cost

Inactive Publication Date: 2016-11-23
JIANGXI TIANREN ECOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The Paenibacillus Kleben activation culture method cannot meet the application of preventing plant diseases in this field, while the traditional fungal fermentation method can realize indus

Method used

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  • Fermentation production method and culture medium of hypocrea virens

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Embodiment 1

[0082] The Paenibacillus clariben strains provided by Jiangxi Tianren Ecological Co., Ltd. were inoculated with 1% of the inoculum on the solid slant of the improved solid Chapei medium II, and cultivated for 3 days at 30°C. activation. Inoculate the activated bacteria into the first-level liquid medium according to the inoculation amount of 2%, and cultivate it at 30°C for 30 hours. During the cultivation process, light is added, and the light intensity is 3000lx. Within 24 hours of cultivation, the ventilation volume is 18m 3 / h, after 24 hours of culture, the ventilation rate is 20m 3 / h, at the same time, the cultivation process needs to be stirred under the condition of stirring once per hour, and each stirring lasts for 20 minutes. The stirring speed is 150-300r / min. , dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.05%, potassium chloride 0.05%, iron sulfate 0.001%, the balance is water, and the initial pH value of the culture is 7.5. After finishing the pri...

Embodiment 2

[0084] The Paenibacillus clariben strains provided by Jiangxi Tianren Ecological Co., Ltd. were inoculated with 1% of the inoculum on the solid slant of the improved solid Chapei medium II, and cultivated for 3 days at 30°C. activation. Inoculate the activated bacteria into the first-level liquid medium according to the inoculum amount of 1.5%, and cultivate it at 32°C for 28 hours. The cultivation process is carried out under the light, and the light intensity is 4000lx. , into the culture within 24h, the ventilation volume is 10m 3 / h, after 24 hours of culture, the ventilation rate is 15m 3 / h, while the cultivation process needs to be stirred under the condition of stirring once per hour, and each stirring lasts for 20min. 0.1%, dipotassium hydrogen phosphate 0.2%, magnesium sulfate 0.03%, potassium chloride 0.1%, iron sulfate 0.001%, the balance is water, and the initial pH value of the culture is 7.0. After finishing the primary culture, inoculate the obtained primary...

Embodiment 3

[0086] The Paenibacillus clariben strains provided by Jiangxi Tianren Ecological Co., Ltd. were inoculated with 1% of the inoculum on the solid slant of the improved solid Chapei medium II, and cultivated for 3 days at 30°C. activation. Inoculate the activated bacteria into the first-level liquid medium according to the inoculation amount of 2.5%, and cultivate it at 28°C for 32 hours. The cultivation process is carried out under the light, and the light intensity is 3500lx. , into the culture within 24h, the ventilation volume is 20m 3 / h, after 24 hours of culture, the ventilation rate is 22m 3 / h, the cultivation process needs to be stirred under the condition of stirring once per hour, and each stirring lasts for 20min. The stirring speed is 150~300r / min. 0.2%, dipotassium hydrogen phosphate 0.08%, magnesium sulfate 0.1%, potassium chloride 0.02%, iron sulfate 0.003%, the balance is water, and the initial pH value of the culture is 8.0. After finishing the primary cultu...

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Abstract

The invention provides a fermentation production method of hypocrea virens. Strains of the hypocrea virens are activated and are cultured in a first-stage seed liquid culture medium and a second-stage liquid culture medium so as to expand reproduction, so as to obtain strong thallus and spores, and second-stage seed liquid is inoculated to a third-stage liquid culture medium formed by a composite carbon source and a nitrogen source, so that the hypocrea virens quickly grows and generates spores. The third-stage liquid culture medium is prepared from the following components according to mass percent: 2.00 to 3.00% of glucose, 0.50 to 1.50% of corn flour, 0.03 to 0.10% of ammonium sulfate, 0.03 to 0.10% of dipotassium phosphate, 0.07 to 0.20% of calcium chloride and the balance of water. The viable count in obtained fermentation liquid is 1 to 5 billion CFU/ml, and the yield of bacterial powder is 10% to 15% after spray drying. By adopting the technical scheme, hypocrea virens powder with high spore content can be obtained at last.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a fermentation production method of Paenibacillus Klibben and a culture medium of Paenibacillus Klibben. Background technique [0002] Paenibacillus Kleben (Hypocrea virens) is a hyperparasitic bacterium that inhibits a variety of soil-borne phytopathogenic fungi. The colony grows rapidly, and after 4 days of cultivation, the diameter is 7-9.5cm. The aerial hyphae are curly, white or gray . Conidiospores are abundant and nearly spread flat on the flat plate. As the main mechanism of biological control of plant diseases, Paenibacillus kliben has been paid more and more attention by plant pathologists in various countries. As an important factor of biological control, hyperparasites play an important role in the control of plant diseases. [0003] In the prior art, there are few reports on the fermentation process of Paenibacillus Klibben. Only the invention paten...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 罗双燕李英武罗建辉梁小文王根豪严艺波李肖宇吴俊杰石峥曾升华石仕福
Owner JIANGXI TIANREN ECOLOGY
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