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A kind of culture medium and application of bacillus amyloliquefaciens

A technology for dissolving starch spores and fermenting medium, applied in the field of microorganisms, can solve the problems of affecting the use effect of preparations, long spore formation time, and low spore formation rate, and achieve the effects of shortening production time, stable product quality and long shelf life.

Active Publication Date: 2015-10-21
内蒙古永太化学有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the inventor found during the research process that the liquid bacterial agent prepared by the method disclosed in the application number 201310070624.6 has a low sporulation rate and a long time for sporulation
If the spore formation rate in the liquid microbial agent is low, there will be problems such as high bacterial mortality, low product viable bacteria, unstable quality, and short shelf life in the subsequent processing of microbial preparations such as adsorption and drying, which will seriously affect the preparation. The effect of using

Method used

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  • A kind of culture medium and application of bacillus amyloliquefaciens
  • A kind of culture medium and application of bacillus amyloliquefaciens

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Experimental program
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Effect test

Embodiment 1

[0014] Embodiment 1 Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) Cultivation of K-8

[0015] (1) Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) Activation of K-8 and preparation of seed solution

[0016] Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) K-8 activation: from Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) K-8 freeze-drying tube, use a sterile inoculation needle to pick part of the bacterial powder into an LB shake flask (250mL shake flask, LB liquid medium filling volume: 50ml), and incubate at 30°C, 150r / min for 24h Afterwards, the LB medium became turbid. Take the bacterial liquid and put it into the LB solid medium for streak culture for 24h-36h, Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) K-8 grows well on LB solid medium, the colony is white or off-white, the surface is irregular and opaque.

[0017] Preparation of the primary seed solution: pick a single colony on the LB solid medium and inoc...

Embodiment 2

[0040] The synergy of embodiment 2 inorganic salts

[0041] In order to investigate the influence of some inorganic salts in the fermentation broth on the fermentation of bacteria and the formation of spores, this comparative experiment was specially designed.

[0042] The basal medium used in the comparative test contains: glucose 5g / L, molasses 20g / L, silkworm chrysalis powder 5g / L, corn steep liquor 15g / L, sodium chloride 15g / L, calcium carbonate 5g / L, pH7.0~7.5 .

[0043] The cultivation conditions were also the same:

[0044] Tank pressure 0.01MPa;

[0045] 0h-8h: The gas-liquid ratio is 0.5:1, and the temperature is 28°C;

[0046] 8h-24h: The gas-liquid ratio is 1:1, and the temperature is 30°C;

[0047] After 24 hours: the gas-liquid ratio is 0.8:1, and the temperature is 32°C.

[0048] The other ingredients and contents added in each test are as follows:

[0049] Test 5: Magnesium sulfate 0.5g / L, manganese sulfate 0.05g / L, ferrous chloride 0.04g / L.

[0050] ...

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Abstract

The invention provides a culture medium and application of Bacillus amyloliquefaciens, and relates to the field of microorganisms. The fermentation medium contains 5-10 g / L of glucose, 10-30 g / L of molasses, 5-10 g / L of silkworm chrysalis powder, 5-20 g / L of corn steep liquor, 10-20 g / L of sodium chloride, and 1-10 g of calcium carbonate / L, magnesium sulfate 0.1~1g / L, manganese sulfate 0.01~0.1g / L, ferrous chloride 0.02~0.08g / L, pH7.0~7.5. Use this medium to cultivate Bacillus amyloliquefaciens K-8, and control the culture conditions as follows: 0h-8h: gas-liquid ratio (0.4-0.6): 1, temperature 25°C-28°C; 8h-24h : The gas-liquid ratio is (0.9~1.1):1, the temperature is 28℃~30℃; after 24 hours: the gas-liquid ratio is (0.7~0.9):1, the temperature is 30℃~32℃. Adopting the culture medium and the culture method of the present invention is beneficial to the mass reproduction of bacillus amyloliquefaciens K-8 in a relatively short period of time, so that a large number of spores can be formed in a relatively short period of time.

Description

technical field [0001] The present invention relates to the field of microorganisms, more specifically to a kind of Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) K-8 culture medium and its application. Background technique [0002] Bacillus amyloliquefaciens is a class of biocontrol bacteria that has been studied a lot and has a wide antibacterial spectrum. Researchers from all over the world have carried out a lot of research work on it in biological control, growth promotion, fertilizer and so on. [0003] The invention patent with the application number 201310070624.6 discloses the understanding of Bacillus amylobacter ( bacillus amyloliquefaciens ) K-8, can simultaneously inhibit a variety of crop pathogens, such as tomato bacterial wilt pathogen, cucumber angular leaf spot pathogen, kiwifruit canker pathogen, Chinese cabbage soft rot pathogen and other bacterial diseases, as well as watermelon wilt pathogen, rice sheath blight Fungal diseases such as the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/07
Inventor 李际军魏鸿刚王琦温学标赵福兵
Owner 内蒙古永太化学有限公司
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