A kind of culture medium and application of bacillus amyloliquefaciens
A technology for dissolving starch spores and fermenting medium, applied in the field of microorganisms, can solve the problems of affecting the use effect of preparations, long spore formation time, and low spore formation rate, and achieve the effects of shortening production time, stable product quality and long shelf life.
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Embodiment 1
[0014] Embodiment 1 Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) Cultivation of K-8
[0015] (1) Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) Activation of K-8 and preparation of seed solution
[0016] Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) K-8 activation: from Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) K-8 freeze-drying tube, use a sterile inoculation needle to pick part of the bacterial powder into an LB shake flask (250mL shake flask, LB liquid medium filling volume: 50ml), and incubate at 30°C, 150r / min for 24h Afterwards, the LB medium became turbid. Take the bacterial liquid and put it into the LB solid medium for streak culture for 24h-36h, Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) K-8 grows well on LB solid medium, the colony is white or off-white, the surface is irregular and opaque.
[0017] Preparation of the primary seed solution: pick a single colony on the LB solid medium and inoc...
Embodiment 2
[0040] The synergy of embodiment 2 inorganic salts
[0041] In order to investigate the influence of some inorganic salts in the fermentation broth on the fermentation of bacteria and the formation of spores, this comparative experiment was specially designed.
[0042] The basal medium used in the comparative test contains: glucose 5g / L, molasses 20g / L, silkworm chrysalis powder 5g / L, corn steep liquor 15g / L, sodium chloride 15g / L, calcium carbonate 5g / L, pH7.0~7.5 .
[0043] The cultivation conditions were also the same:
[0044] Tank pressure 0.01MPa;
[0045] 0h-8h: The gas-liquid ratio is 0.5:1, and the temperature is 28°C;
[0046] 8h-24h: The gas-liquid ratio is 1:1, and the temperature is 30°C;
[0047] After 24 hours: the gas-liquid ratio is 0.8:1, and the temperature is 32°C.
[0048] The other ingredients and contents added in each test are as follows:
[0049] Test 5: Magnesium sulfate 0.5g / L, manganese sulfate 0.05g / L, ferrous chloride 0.04g / L.
[0050] ...
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