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Streptomyces jingyang wettable powder and preparation method thereof

A technology of Streptomyces jingyang and wettable powder, which is applied in the field of agricultural microorganisms and biological pesticides, and can solve the problems of obtaining fertile soil that is not as convenient and stable as before, low in bacteria content, and unable to be killed

Active Publication Date: 2019-09-10
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the preparation method of the wettable powder of Streptomyces jingyang has not been reported yet. The cultivation and research of Streptomyces jingyang were studied more in the 1960s and 1970s, and most of them were fermented in fertile soil, but we found through experiments that After more than 30 years of re-application of chemical fertilizers and less application of organic fertilizers in most of the soil in China, the acquisition of fertilizer soil is not as convenient and stable as before. We have carried out fermentation tests on land samples obtained from more than 20 provinces across the country. , it was found that after fermentation using the existing technology, the highest spore content of Streptomyces jingyang did not exceed 2 billion CFU / g, and a large number of pests would remain in the fertilizer soil fermentation, which could not be killed. If it is applied to healthy soil It will cause the spread of pests, and the fertile soil is not suitable as a carrier for wettable powder
The existing Streptomyces wettable powder has too low bacterial content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A Streptomyces Jingyang wettable powder is characterized in that, the Streptomyces Jingyang wettable powder is composed of the following parts by weight: 90 parts of Streptomyces Jingyang fermentation bacteria powder; wetting agent: 3 parts of sodium dodecylbenzenesulfonate ; Dispersant: 0.7 parts of tea saponin; UV protection agent: 1.5 parts of ascorbic acid, 0.6 part of β-dextrin; dry protection agent: 3 parts of maltose, 0.8 part of mannitol; its specific preparation method consists of the following steps:

[0022] One, the preparation of Streptomyces jingyang fermentation bacteria powder

[0023]Take out the strain preservation tube, scratch the plate with Gaoshi No. 1 solid medium for recovery, culture at 30°C for 4-5 days, pick a single colony under the plate and inoculate it on the Gaoshi No. 1 solid medium in the eggplant bottle, and spread it all over the place. Eggplant bottle, incubate in an incubator at 30°C for 4-6 days, wait until there are light pink spo...

Embodiment 2

[0034] Application effect of Streptomyces jingyang wettable powder of the present invention in the control of banana wilt

[0035] 1 Test time: May 2014-June 2016

[0036] 2. Test location: a farm in Xuwen County, Guangdong Province

[0037] 3. Test plan: test group: drip irrigation once a month, the dosage is 1 kg / mu of Streptomyces jingyang wettable powder of the present invention, and the use area is 22 mu; control group: use a commercially available Bacillus wettable powder (special for bananas) 10 kilograms / mu, use area is 108 mus, control group uses bacillus wettable powder to use until March, 2015, also uses Streptomyces jingyang wettable powder of the present invention from April, 2015; Other management is identical.

[0038] 4. test results:

[0039] We have counted the morbidity situation of test group and matched group, the results are as follows in table 1, as can be seen clearly from table 1, test group uses the morbidity rate of Streptomyces jingyang wettabl...

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PUM

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Abstract

The invention provides a preparation method of Streptomyces jingyangensis wettable powder. The method comprises the following steps: fermenting a carrier kaolin, Rhodopseudomonas palustris, and nutrients corn starch and bean pulp powder to obtain high-concentration Streptomyces jingyangensis powder; and mixing 90-95 parts of Streptomyces jingyangensis fermented powder, 1-4 parts of wetting agent sodium dodecyl benzene sulfonate, 0.2-1 part of dispersing agent tea saponin, 0.5-2 parts of ultraviolet protective agent ascorbic acid, 0.2-1 part of beta-dextrin, 1-4 parts of drying protective agent maltose and 0.2-1 part of mannitol to obtain the stable Streptomyces jingyangensis wettable powder with the bacterium content of more than 10 billion CFU / g.

Description

technical field [0001] The invention relates to a preparation method of Streptomyces jingyang wettable powder, which belongs to the technical field of agricultural microorganisms and biological pesticides. Background technique [0002] Streptomyces jingyangensis is an actinomycetes, commonly known as 5406. The metabolic activity of Streptomyces jingyang can promote the decomposition of organic matter, make nutrients effective, and release antibacterial substances, which can effectively prevent various crop diseases and insect pests, and can produce hormones that stimulate plant growth. It can also promote the formation of soil aggregates , has the ability to promote the formation of humus and produce polysaccharides. This antibiotic has been widely used on a variety of crops. Streptomyces is the most valuable actinomycete for biocontrol, and many species in the genus Streptomyces have antagonistic effects on plant pathogens. At present, more than 10 kinds of Streptomyces ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01N25/14A01P1/00
CPCA01N25/14A01N63/10
Inventor 蒋常德胡艳晖
Owner FOSHAN YANHUI BIOTECH CO LTD
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