A kind of method for cultivating Bacillus brevis
A microbacillus, short-term technology, applied in the field of culturing small bacillus, can solve the problems of long fermentation time, insufficient live bacteria content and spore content
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Embodiment 1
[0013] Embodiment 1 adopts culture medium of the present invention to cultivate Microbacillus pumilus TW
[0014] Use a sterile toothpick to pick out a single colony of Microbacillus brevis TW that has been streaked and cultured on LB solid medium in advance, inoculate it in a 20mL Erlenmeyer flask containing 5mL of PDA liquid culture medium, and incubate at 30°C and 200rpm Cultivate under shaking for 16 hours to obtain the primary seed liquid. Inoculate all 5 mL of the obtained seed liquid into a 1000 mL Erlenmeyer flask containing 400 mL of PDA culture liquid, and shake and culture at 30°C and 200 rpm for 16 hours to obtain a secondary seed liquid. The resulting 400 mL culture solution was inoculated into 20 L of fermentation medium 1.
[0015] Fermentation medium 1, containing the following components: molasses 6g / L, cornstarch 8g / L, soybean cake powder 15g / L, corn steep liquor 10g / L, yeast powder 5g / L, ammonium chloride 4.5g / L, calcium carbonate 5g / L, K 2 HPO 4 ·3H 2 ...
Embodiment 2
[0028] Use a sterile toothpick to pick out a single colony of Microbacillus brevis TW that has been streaked and cultured on LB solid medium in advance, and inoculate it in a 20mL Erlenmeyer flask containing 5mL of PDA liquid medium. Cultivate under shaking for 16 hours to obtain the primary seed liquid. All the 5 mL of seed liquid obtained was inoculated into a 1000 mL Erlenmeyer flask containing 400 mL of PDA liquid medium, and cultured with shaking at 30°C and 200 rpm for 16 hours to obtain a secondary seed liquid. The resulting 400 mL culture solution was inoculated into 20 L fermentation medium 2.
[0029] Fermentation medium 2 contains the following components: molasses 5g / L, cornstarch 9g / L, soybean cake powder 14g / L, corn steep liquor 9g / L, yeast powder 6g / L, ammonium chloride 5g / L, calcium carbonate 4.5 g / L,K 2 HPO 4 ·3H 2 O1.2g / L, ZnSO 4 ·7H 2 O1.8g / L, pH7.0, solvent is water. Fermentation conditions: control the pH of the fermentation broth to 7.0-7.2, dissol...
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