A kind of method for cultivating Bacillus brevis

A microbacillus, short-term technology, applied in the field of culturing small bacillus, can solve the problems of long fermentation time, insufficient live bacteria content and spore content

Active Publication Date: 2020-10-09
内蒙古永太化学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Bacillus brevis TW has a good control effect on rice blast, but the fermentation time is too long, and the content of live bacteria and spores in the fermentation liquid is not high enough

Method used

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  • A kind of method for cultivating Bacillus brevis
  • A kind of method for cultivating Bacillus brevis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1 adopts culture medium of the present invention to cultivate Microbacillus pumilus TW

[0014] Use a sterile toothpick to pick out a single colony of Microbacillus brevis TW that has been streaked and cultured on LB solid medium in advance, inoculate it in a 20mL Erlenmeyer flask containing 5mL of PDA liquid culture medium, and incubate at 30°C and 200rpm Cultivate under shaking for 16 hours to obtain the primary seed liquid. Inoculate all 5 mL of the obtained seed liquid into a 1000 mL Erlenmeyer flask containing 400 mL of PDA culture liquid, and shake and culture at 30°C and 200 rpm for 16 hours to obtain a secondary seed liquid. The resulting 400 mL culture solution was inoculated into 20 L of fermentation medium 1.

[0015] Fermentation medium 1, containing the following components: molasses 6g / L, cornstarch 8g / L, soybean cake powder 15g / L, corn steep liquor 10g / L, yeast powder 5g / L, ammonium chloride 4.5g / L, calcium carbonate 5g / L, K 2 HPO 4 ·3H 2 ...

Embodiment 2

[0028] Use a sterile toothpick to pick out a single colony of Microbacillus brevis TW that has been streaked and cultured on LB solid medium in advance, and inoculate it in a 20mL Erlenmeyer flask containing 5mL of PDA liquid medium. Cultivate under shaking for 16 hours to obtain the primary seed liquid. All the 5 mL of seed liquid obtained was inoculated into a 1000 mL Erlenmeyer flask containing 400 mL of PDA liquid medium, and cultured with shaking at 30°C and 200 rpm for 16 hours to obtain a secondary seed liquid. The resulting 400 mL culture solution was inoculated into 20 L fermentation medium 2.

[0029] Fermentation medium 2 contains the following components: molasses 5g / L, cornstarch 9g / L, soybean cake powder 14g / L, corn steep liquor 9g / L, yeast powder 6g / L, ammonium chloride 5g / L, calcium carbonate 4.5 g / L,K 2 HPO 4 ·3H 2 O1.2g / L, ZnSO 4 ·7H 2 O1.8g / L, pH7.0, solvent is water. Fermentation conditions: control the pH of the fermentation broth to 7.0-7.2, dissol...

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Abstract

The invention provides a method for culturing brevibacillus brevis and relates to the field of bio-pesticides. The method culturing the brevibacillus brevis by a fermentation medium containing the following components: 5-7 g / L of molasses, 7-9 g / L of corn starch, 14-16 g / L of soybean cake meal, 9-11 g / L of corn steep liquor, 4-6 g / L of yeast powder, 4-5 g / L of ammonium chloride, 4.5-5 g / L of calcium carbonate, 1.2-1.7 g / L of dipotassium phosphate, 1.8-2.2 g / L of zinc sulfate, and the pH is 7.0-7.5. The method for culturing brevibacillus brevis can obtain more viable bacteria in a shorter time,and the spore content is higher.

Description

technical field [0001] The invention relates to the field of biological pesticides, in particular to a method for cultivating Microbacillus brevis. Background technique [0002] Rice blast is one of the most serious rice diseases worldwide, causing a 10-20% reduction in rice yield. Rice blast can seriously affect rice throughout the year, harming various parts of rice, including seedling blast, leaf blast, and leaf pillow blast. [0003] Bacillus brevis TW has a good control effect on rice blast, but the fermentation time is too long, and the content of live bacteria and spores in the fermentation liquid is not high enough. Contents of the invention [0004] The object of the present invention is to provide a method for cultivating Bacillus pumilus, which can obtain more viable bacteria in a shorter period of time, and has a higher content of spores. [0005] The object of the present invention adopts following technical scheme to realize. [0006] A method for cultivat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 李燕赵福兵孙海勇
Owner 内蒙古永太化学有限公司
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