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Gene recombination bacillus amyloliquefaciens and microbial inoculum preparation method

A technology of amylolytic spores and gene recombination, which is applied in the field of plant disease-resistant and growth-promoting engineering bacteria and the preparation of bacterial agents, which can solve problems affecting the growth and colonization of bacteria, affecting the effect of disease prevention and growth promotion, and achieve antibacterial lipopeptide High yield, good antibacterial effect, and high genetic stability

Inactive Publication Date: 2010-06-02
HUNAN NORMAL UNIVERSITY
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Problems solved by technology

In addition, after being applied to the soil, if the oxygen content in the soil is low, it will affect the growth and colonization ability of the bacteria, and then affect its disease prevention and growth promotion effect

Method used

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  • Gene recombination bacillus amyloliquefaciens and microbial inoculum preparation method
  • Gene recombination bacillus amyloliquefaciens and microbial inoculum preparation method
  • Gene recombination bacillus amyloliquefaciens and microbial inoculum preparation method

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Embodiment Construction

[0056] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.

[0057] In the embodiment of the present invention, the plant rhizosphere growth-promoting pathogen Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) FZB42 is used as the recipient bacterium to integrate the vgb gene vgb of Vitiligo hyaline into the amyloliquefaciens by constructing the gene recombination integration vector pDGP43-vgb Achieve high expression in Bacillus, construct Bacillus amyloliquefaciens engineering bacteria strain XLV09-1 with higher spore number and antibacterial lipopeptide production, and prepare Bacillus amyloliquefaciens engineering bacteria strain.

[0058] 1. Example of the construction method of genetically recombined Bacillus amyloliquefaciens engineering bacteria

[0059] (1) PCR amplification of P 43 Promoter

[0060] Extract the total DNA of Bacillus subtilis B. subtilis 168 strain, method reference [Iso...

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Abstract

The invention discloses gene recombination bacillus amyloliquefaciens and a microbial inoculum preparation method. A bacillus amyloliquefaciens FZB42 strain is taken as a host bacterium to construct the gene recombination bacillus amyloliquefaciens XLV09-1 with transparent vitreoscilla hemoglobin which is regulated and controlled by a P43 strong promoter by contrasting a gene recombination vector. The produced spore number of microbial inoculum prepared by fermenting the bacillus amyloliquefaciens can reach more than 100 hundred millions per milliliter, the overall yield of antibacterial lipopeptid reaches more than 1 gram per liter and prevention effect on various fungal plant diseases is improved by over 30 percent. The microbial inoculum is a green microecological preparation, of no toxic residue, safe to human and livestock, and favorable for protecting the ecological environment.

Description

technical field [0001] The invention relates to a plant disease-resistant and growth-promoting engineering bacterium and a preparation method of a bacterial agent, in particular to a genetically recombined Bacillus amyloliquefaciens containing the hemoglobin gene of Vitella hyaline and a preparation method of a bacterial agent. Background technique [0002] In agricultural production, the use of chemical pesticides and chemical fertilizers is increasing year by year, causing problems such as environmental pollution, ecological deterioration, soil compaction and soil degradation, and poses a huge threat and challenge to food safety, human health and sustainable agricultural development. Therefore, to develop sustainable agriculture, it is necessary to reduce the use of chemical pesticides and chemical fertilizers and find effective alternatives. [0003] Microbial pesticides and microbial fertilizers have the characteristics of long-lasting effect, no pollution to the environ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12N15/31C12N15/75C12P21/02C12R1/07
Inventor 夏立秋丁学知刘清术余子全胡胜标孙运军
Owner HUNAN NORMAL UNIVERSITY
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