Gene recombination bacillus amyloliquefaciens and microbial inoculum preparation method

A technology of amylolytic spores and gene recombination, applied in botany equipment and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problem of affecting the growth and colonization ability of bacteria, affecting the effect of disease prevention and growth promotion, etc. problem, to achieve the effect of high antibacterial lipopeptide production, good antibacterial effect and high genetic stability
CN101717744BInactive Publication Date: 2012-05-23HUNAN NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
HUNAN NORMAL UNIVERSITY
Publication Date
2012-05-23
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses gene recombination bacillus amyloliquefaciens and a microbial inoculum preparation method. A bacillus amyloliquefaciens FZB42 strain is taken as a host bacterium to construct the gene recombination bacillus amyloliquefaciens XLV09-1 with transparent vitreoscilla hemoglobin which is regulated and controlled by a P43 strong promoter by contrasting a gene recombination vector. The produced spore number of microbial inoculum prepared by fermenting the bacillus amyloliquefaciens can reach more than 100 hundred millions per milliliter, the overall yield of antibacterial lipopeptid reaches more than 1 gram per liter and prevention effect on various fungal plant diseases is improved by over 30 percent. The microbial inoculum is a green microecological preparation, of no toxic residue, safe to human and livestock, and favorable for protecting the ecological environment.
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Description

technical field

[0001] The invention relates to a plant disease-resistant and growth-promoting engineering bacterium and a preparation method of a bacterial agent, in particular to a genetically recombined Bacillus amyloliquefaciens containing a hyaline hemoglobin gene and a preparation method of a bacterial agent. Background technique

[0002] In agricultural production, the use of chemical pesticides and chemical fertilizers is increasing year by year, causing problems such as environmental pollution, ecological deterioration, soil compaction and soil degradation, and poses a huge threat and challenge to food safety, human health and sustainable agricultural development. Therefore, to develop sustainable agriculture, it is necessary to reduce the use of chemical pesticides and chemical fertilizers and find effective alternatives.

[0003] Microbial pesticides and microbial fertilizers have the characteristics of long-lasting effect, no pollution to the environment, and low...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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