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Preparation method and application of coniothyrium minitans ZS-1SB fungicide

A technology of ZS-1SB and C. shield, which is applied in the direction of using spores, botanical equipment and methods, and methods based on microorganisms. It can solve the problems of unsuitability for large-scale production of C. shield and achieve remarkable disease prevention effects. , low price, reduced quantity effect

Inactive Publication Date: 2013-10-30
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The reported methods all have higher requirements on equipment, need autoclaving equipment or special cultivation site (biochemical incubator or fermenter), and are not suitable for large-scale production of shell mold

Method used

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  • Preparation method and application of coniothyrium minitans ZS-1SB fungicide
  • Preparation method and application of coniothyrium minitans ZS-1SB fungicide

Examples

Experimental program
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Embodiment 1

[0025] Embodiment 1: the method for the large-scale mechanized solid fermentation of shell mold ZS-1SB bacterial agent, its step is:

[0026] 1. Preparation of C. shield ZS-1SB spore suspension: place C. shield ZS-1SB strain on the slant of PDA medium (formulation as described above) and culture at 20° C. for 7 days. Rinse the slope with sterile water and adjust the conidia suspension to 10 6 Spores / ml.

[0027] 2. Pre-cultivation of C. shield ZS-1SB: Shake the conidium suspension of C. shield ZS-1SB prepared in step 1 at 150r / min and 20°C for 2 hours to obtain C. shield ZS-1SB bacterial liquid ,spare.

[0028] 3. Large-scale mechanized solid fermentation culture of C. shieldum ZS-1SB inoculum: dry compost (water content is generally no more than 8%) oatmeal (commercially purchased) and chaff (commercially purchased) with a volume ratio of 1:1 Mix evenly, add appropriate amount of water (make it swell, make the water content reach 40%, use edible fungus bagging machine (ZDJ...

Embodiment 2

[0029] Embodiment 2: the application of a kind of biocontrol fungus Clamella shield ZS-1SB bacterial agent in the preparation treatment or prevention rape sclerotinia sclerotinia medicaments, its application process is:

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Abstract

The invention discloses a preparation method and an application of a coniothyrium minitans ZS-1SB fungicide. The preparation method comprises the following steps of: (1) first, performing PDA (Potato Dextrose Agar) slant culture for the coniothyrium minitans ZS-1SB fungicide; washing the slant with sterile water, and adjusting a conidium suspension liquid to 106 spores / ml; (2) oscillating the conidium suspension liquid of coniothyrium minitans ZS-1SB prepared by the step 1 to obtain a coniothyrium minitans ZB-1SB bacterial liquid; and (3) uniformly mixing oatmeal and husks, adding water till the water content reaches 40%, filling by an edible fungus bagging machine, and steam sterilizing at normal pressure; after cooling, inoculating the coniothyrium minitans ZB-1SB bacterial liquid, and culturing in a certain condition by a 30ml / culture bottle. The yield of coniothyrium minitans prepared by the preparation method disclosed by the invention can reach 3.2*10<10> spore / g dry compost. Under field culture condition of an antibiological inoculant, soil broadcast fertilization and overground part in blossom period realizes the preventing effect of 57% to sclerotinia rot of colza. The fungicide can be applied to large-scale disease prevention and treatment.

Description

technical field [0001] The present invention relates to the technical field of microbial pesticides, more specifically to a method for large-scale mechanized solid fermentation of a C. shield ZS-1SB inoculum with biocontrol potential for Sclerotinia sclerotiorum, and also relates to a C. shield ZS-1SB bacterium The application of the agent in the preparation of medicines for treating or preventing Sclerotinia sclerotiorum. Background technique [0002] S. sclerotiorum can infect oilseed rape, soybean, sunflower and other major oil crops and lettuce, Chinese cabbage, celery, carrot and other important vegetable crops, causing sclerotinia of various plants worldwide. Since no planting resources with obvious disease resistance have been found so far, and no disease-resistant varieties can be used, the sclerotinia caused by it is still a worldwide problem in agricultural production. Because chemical pesticides are difficult to reach the damaged parts such as the stems and branc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00A01N63/04A01P3/00C12R1/645
Inventor 姜道宏程家森付艳苹谢甲涛
Owner HUAZHONG AGRI UNIV
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