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A kind of preparation method and application of shell mold zs-1sb ​​bacterial agent

A technology of ZS-1SB, Conchlea scutellum, which is applied in the methods of using spores, botanical equipment and methods, and methods based on microorganisms, etc., can solve problems such as being unsuitable for large-scale production of scutellum scutellum, and achieve a remarkable disease prevention effect. , low price, high spore content

Inactive Publication Date: 2015-12-02
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported methods all have high requirements on equipment, and require high-pressure sterilization equipment or special cultivation sites (biochemical incubators or fermentation tanks), which are not suitable for large-scale production of C. shieldiae

Method used

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  • A kind of preparation method and application of shell mold zs-1sb ​​bacterial agent
  • A kind of preparation method and application of shell mold zs-1sb ​​bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the method for the large-scale mechanized solid fermentation of shell mold ZS-1SB bacterial agent, its step is:

[0026] 1. Preparation of C. shield ZS-1SB spore suspension: put the C. shield ZS-1SB strain on the slant of PDA medium (the formula is as described above), and cultivate it at 20°C for 7 days. Rinse the slope with sterile water and adjust the conidia suspension to 10 6 Spores / ml.

[0027] 2. Pre-cultivation of C. shield ZS-1SB: Shake the conidium suspension of C. shield ZS-1SB prepared in step 1 at 150r / min and 20°C for 2 hours to obtain C. shield ZS-1SB bacterial liquid ,spare.

[0028] 3. Large-scale mechanized solid fermentation culture of C. shieldum ZS-1SB inoculum: dry compost (water content generally not exceeding 8%) oatmeal (commercially purchased) and chaff (commercially purchased) at a volume ratio of 1:1 Mix well, add appropriate amount of water (to make it swell, so that the water content reaches 40%, and use the edible fungi ba...

Embodiment 2

[0029] Embodiment 2: the application of a kind of biocontrol fungus Clamella shield ZS-1SB bacterial agent in the preparation treatment or prevention rape sclerotinia sclerotinia medicaments, its application process is:

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Abstract

The invention discloses a preparation method and an application of a coniothyrium minitans ZS-1SB fungicide. The preparation method comprises the following steps of: (1) first, performing PDA (Potato Dextrose Agar) slant culture for the coniothyrium minitans ZS-1SB fungicide; washing the slant with sterile water, and adjusting a conidium suspension liquid to 106 spores / ml; (2) oscillating the conidium suspension liquid of coniothyrium minitans ZS-1SB prepared by the step 1 to obtain a coniothyrium minitans ZB-1SB bacterial liquid; and (3) uniformly mixing oatmeal and husks, adding water till the water content reaches 40%, filling by an edible fungus bagging machine, and steam sterilizing at normal pressure; after cooling, inoculating the coniothyrium minitans ZB-1SB bacterial liquid, and culturing in a certain condition by a 30ml / culture bottle. The yield of coniothyrium minitans prepared by the preparation method disclosed by the invention can reach 3.2*10<10> spore / g dry compost. Under field culture condition of an antibiological inoculant, soil broadcast fertilization and overground part in blossom period realizes the preventing effect of 57% to sclerotinia rot of colza. The fungicide can be applied to large-scale disease prevention and treatment.

Description

technical field [0001] The present invention relates to the technical field of microbial pesticides, more specifically to a method for large-scale mechanized solid fermentation of a C. shield ZS-1SB inoculum with biocontrol potential for Sclerotinia sclerotiorum, and also relates to a C. shield ZS-1SB bacterium The application of the agent in the preparation of medicines for treating or preventing Sclerotinia sclerotiorum. Background technique [0002] S. sclerotiorum can infect oilseed rape, soybean, sunflower and other major oil crops and lettuce, Chinese cabbage, celery, carrot and other important vegetable crops, causing sclerotinia of various plants worldwide. Since no planting resources with obvious disease resistance have been found so far, and no disease-resistant varieties can be used, the sclerotinia caused by it is still a worldwide problem in agricultural production. Because chemical pesticides are difficult to reach the damaged parts such as the stems and branc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00A01N63/04A01P3/00C12R1/645
Inventor 姜道宏程家森付艳苹谢甲涛
Owner HUAZHONG AGRI UNIV
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