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Production of trichodermin agricultural chemicals

A biopesticide and preparation process technology, applied in the fields of chemicals for biological control, botanical equipment and methods, biocides, etc., can solve the problems of poor adaptability, unstable effect, poor control effect, etc. content, increase the effect of loose permeability

Inactive Publication Date: 2007-09-05
云南生物制药有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the shortcomings of existing Trichoderma preparations such as poor adaptability, poor control effect, and unstable effect, the invention provides a method that can promote the production of chlamydospores, increase the activity and biomass of Trichoderma in soil, and improve the quality of Trichoderma. Proliferation and colonization ability in soil, method for preparing Trichoderma preparation that improves spore content of Trichoderma in solid fermentation product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] 1. Place Trichoderma in the following existing slant medium:

[0061] Potatoes 200g

[0062] Glucose 20g

[0063] Agar 20g

[0064] Add water to 1000ml;

[0065] Cultivate for 4-5 days at a temperature of 25°C; then use the same medium and the same culture conditions for spore bottle propagation;

[0066] 2. Inoculate the Trichoderma culture after the expansion and propagation in the liquid fermentation medium of the following existing mass ratios:

[0067] Molasses 1%

[0068] Yeast powder 0.25%

[0069] NaNO 3 0.7%

[0070] MnCl 2 4H 2 O 0.25%

[0071] MgSO 4 7H 2 O 0.15%

[0072] K H 2 PO 4 0.36%

[0073] The balance is water

[0074] At a temperature of 25°C, according to the amount of 1 liter of medium: 1.5 liters of air, air was introduced to stir, and stirred for 5 minutes every 2 hours, and cultivated for 60 hours;

[0075] 3. Inoculate 15 kilograms of liquid fermentation product into 100 kilogr...

Embodiment 2

[0086] 1. Place Trichoderma in the following slant medium:

[0087] Potatoes 200g

[0088] Glucose 20g

[0089] Agar 18g

[0090] Add water to 1000ml;

[0091] Cultivate for 4-5 days at a temperature of 25°C; then use the same medium and the same culture conditions for spore bottle propagation;

[0092] 2. Inoculate the Trichoderma culture after the expansion and propagation in the liquid fermentation medium of the following mass ratio:

[0093] Molasses 1%

[0094] Yeast powder 0.25%

[0095] NaNO 3 0.7%

[0096] MnCl 2 4H 2 O 0.25%

[0097] MgSO 4 7H 2 O 0.15%

[0098] K H 2 PO 4 0.36%

[0099] The balance is water

[0100] At a temperature of 28°C, according to the amount of 1 liter of medium: 2 liters of air, air was introduced to stir, and stirred for 3 minutes every 3 hours, and cultivated for 48 hours;

[0101] 3. Inoculate 25 kilograms of liquid fermentation product into 100 kilograms of solid fermenta...

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PUM

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Abstract

A process for preparing the biologic agricultural chemical from trichoderma includes such steps as reproductive culture of trichoderma, fermenting in liquid, inoculating it in the culture medium prepared from cane dregs, wheat bran and rice husk for solid fermenting, culturing at 25-28 deg.C under 60-80% of relative humidity for 4-5 days, culturing at low temp (lower than 12 deg.C) for 2-3 days, drying at 40-50 deg.C for 12-24 hr, adding promoter prepared from diatomite, wheat bran and sulfur, mixing, pulverizing and sieving.

Description

technical field [0001] The invention relates to a preparation process of a trichoderma biological pesticide, in particular to a preparation method of a microbial pesticide for preventing and treating crop root rot, and belongs to the technical field of biological pesticides. Background technique [0002] Plant root rot caused by pathogens such as Rhizoctonia solani, Sclerotium rolfsii, Pythium spp., Fusarium spp., and Phytophthoraspp. It is an important cause of root and even whole plant rot and death, and it is also a kind of plant disease that is widely distributed, seriously harmful, and difficult to control. The disease is very serious to food crops, commercial crops and trees. Commonly used chemical control tools remain, polluting food and the environment. Trichoderma spp. is an important class of antagonistic microorganisms, which have obvious inhibitory effects on the above-mentioned plant pathogenic fungi. The biocontrol mechanisms of Trichoderma against phytopath...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P1/00
Inventor 武宽刘云龙徐同徐永华李文春张建国
Owner 云南生物制药有限公司
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