A method for efficiently improving the germination rate of Bacillus subtilis

A technology of Bacillus subtilis and germination rate, applied in the field of microbial preparation, can solve problems such as low spore content, and achieve the effects of improving activity, low cost and simple fermentation method

Inactive Publication Date: 2016-05-25
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defect of low spore content in bacillus microbial preparation products in the prior art, and provide a method for efficiently improving the germination rate of bacillus subtilis

Method used

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  • A method for efficiently improving the germination rate of Bacillus subtilis

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Embodiment 1

[0038] The strain used in this embodiment is Bacillus subtilis ( Bacillus subtilis ), Bacillus subtilis purchased from China General Microorganism Culture Collection Center, number CGMCC1.433.

[0039] The fermentation preparation method of bacillus product comprises the following steps:

[0040] (1) Preparation of strain liquid medium: 5g glucose, 5g sodium chloride, 3g peptone and 1L water (pH value 6.5); prepare three parts;

[0041] Preparation of seed rejuvenation liquid medium: 5g glucose, 5g sodium chloride, 3g peptone, 6g soybean powder, 12g food grade isomaltooligosaccharide, 10g food grade soybean oligosaccharide and 1L water (pH value is 6.5), prepare a share;

[0042] Add 2% agar to a portion of the above strain liquid culture medium and pour it into a sterile test tube to make a solid slant medium for seeds;

[0043] Add 2% agar to another part of the above-mentioned bacterial strain liquid medium, pour it into a sterile plate, and make a solid plate medium for...

Embodiment 2

[0059] The strain used in this embodiment is Bacillus subtilis ( Bacillus subtilis ) 1 strain, Bacillus subtilis purchased from China General Microorganism Culture Collection Center, No. CGMCC1.504.

[0060] The fermentation preparation method of bacillus product comprises the following steps:

[0061] (1) Preparation of bacterial culture medium: 3g glucose, 3g sodium chloride, 3g peptone and 1L water (pH value 6.5); prepare three parts;

[0062] Preparation of seed rejuvenation liquid medium: 3g glucose, 3g sodium chloride, 5g peptone, 5g soybean flour, 10g food grade isomaltooligosaccharide, 8g food grade soybean oligosaccharide and 1L water (pH value is 6.5), prepare a share;

[0063] Add 2% agar to a portion of the above strain liquid culture medium and pour it into a sterile test tube to make a solid slant medium for seeds;

[0064] Add 2% agar to another part of the above-mentioned bacterial strain liquid medium, pour it into a sterile plate, and make a solid plate me...

Embodiment 3

[0080]The strain used in this embodiment is Bacillus subtilis ( Bacillus subtilis ), Bacillus subtilis purchased from China General Microorganism Culture Collection Center, number CGMCC1.460.

[0081] The fermentation preparation method of bacillus product comprises the following steps:

[0082] (1) Preparation of bacterial culture medium: 4g glucose, 3.5g sodium chloride, 4g peptone and 1L water (pH value 7); prepare three parts;

[0083] The preparation of seed rejuvenation liquid medium: 4g glucose, 4g sodium chloride, 2g peptone, 5.5g soybean powder, 11g food grade isomaltooligosaccharide, 8.5g food grade soybean oligosaccharide and 1L water (pH value is 7), Prepare a copy;

[0084] Add 2% agar to a portion of the above strain liquid culture medium and pour it into a sterile test tube to make a solid slant medium for seeds;

[0085] Add 2% agar to another part of the above-mentioned bacterial strain liquid medium, pour it into a sterile plate, and make a solid plate med...

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Abstract

The invention relates to the technical field of preparation of microbes and specifically relates to a method for efficiently improving the germination rate of Bacillus subtilis. According to the method provided by the invention, glycerine is added in a Bacillus subtilis fermentation broth as a fermentation strain, an inorganic salt solution capable of improving the spore germination rate is added when the fermentation strain is cultured for 15-18 hours, and the inorganic salt solution contains the following components in percentage by weight: 0.12-0.125% of MgSO4.7H2O, 0.01-0.015% of MnSO4.H2O, 0.05-0.1% of CaCl2, 0.005-0.008% of FeSO4.7H2O, 0.015-0.02% of KI and 0.035-0.04% of KH2PO4. The fermentation of the strain obtained by the method can achieve 5.8*10<11> CFU / ml, the number of spores is 5.49*10<11> CUF / ml, and the spore germination rate is 94.7%.

Description

technical field [0001] The invention relates to the technical field of microorganism preparation, more specifically, to a method for efficiently improving the germination rate of Bacillus subtilis. Background technique [0002] Bacillus subtilis is a kind of Bacillus, its thallus is rod-shaped, the colony surface is rough and opaque, dirty white or slightly yellow. Bacillus subtilis can form spores and is an important industrial enzyme production bacterium. It can be used to produce α-amylase, protease, β-glucanase, alkaline phosphatase, etc. In addition, the bacteria of Bacillus subtilis can be It is directly used in biological pesticides, so it has a wide range of applications and application value. [0003] Bacillus is a stress-resistant dormant body formed during the growth of spore-forming bacteria. Compared with its vegetative cells, spores of Bacillus subtilis are easier to preserve and have a higher resurrection rate. They are ideal forms of existence for preparing ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/125
Inventor 张玲华邝哲师蔡海明赵祥杰楚品品叶明强
Owner SOUTH CHINA AGRI UNIV
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