Fermentation production method for hypocrea virens and solid medium of hypocrea virens

A solid medium and solid culture technology, applied in the field of microbial fermentation, can solve the problems of high cost, unsatisfactory, long fermentation time, etc.

Inactive Publication Date: 2016-11-23
JIANGXI TIANREN ECOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The activation culture method of Hypocreas chlorogenes cannot meet the application of preventing plant diseases in this field, while the traditional fungal fermentation method can realize ind

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] The H. chlorogenic strain provided by Jiangxi Tianren Ecological Co., Ltd. was inoculated with 1% on the PDA solid slant medium, and cultured at 28°C for 3 days to complete the activation of the strain. The activated H. chlorogenic strains were inoculated into a 250L fermenter equipped with a primary seed medium for liquid cultivation for 40 hours according to the inoculation amount of 1%. The culture temperature was 27°C and the initial pH value was 6.0. The primary seed medium The formula includes 1% of glucose, 1% of white sugar, 1% of soybean powder, 0.2% of ammonium sulfate, 0.2% of dipotassium hydrogen phosphate, 0.08% of potassium chloride, 0.02% of magnesium sulfate, 0.0002% of iron sulfate, and the balance is water. 24h after cultivation, according to 15m 3 / h ventilation for aeration culture, after 24 hours of liquid culture, according to 24m 3 The aeration rate per hour was used for aeration culture, the temperature of the liquid culture process was controll...

Embodiment 2

[0070] The H. chlorogenic strain provided by Jiangxi Tianren Ecological Co., Ltd. was inoculated with 0.8% on the PDA solid slant medium, and activated at 30°C for 4 days to complete the activation of the strain. The activated H. chlorogenic strains were inoculated into a 250L fermenter equipped with a primary seed medium for liquid cultivation for 50 hours according to an inoculation amount of 1.5%. The culture temperature was 28°C and the initial pH value was 6.5. The primary seed medium The formula includes 0.5% of glucose, 2.0% of white sugar, 0.3% of soybean powder, 0.1% of ammonium sulfate, 0.2% of dipotassium hydrogen phosphate, 0.05% of potassium chloride, 0.04% of magnesium sulfate, 0.0008% of iron sulfate, and the balance is water. 24 hours after cultivation, according to 10m 3 / h ventilation for aeration culture, after 24 hours of liquid culture, according to 15m 3 The aeration rate per hour was used for aeration culture, the temperature of the liquid culture proce...

Embodiment 3

[0072] The H. chlorogenic strain provided by Jiangxi Tianren Ecological Co., Ltd. was inoculated with 1.0% on the PDA solid slant medium, and activated at 30°C for 3 days to complete the activation of the strain. The activated H. chlorogenic strains were inoculated into a 250L fermenter equipped with a primary seed medium for liquid cultivation for 35 hours according to an inoculation amount of 1%. The culture temperature was 32° C., and the initial pH value was 5.5. The formula includes 2.00% of glucose, 0.50% of white sugar, 0.10% of soybean powder, 0.30% of ammonium sulfate, 0.05% of dipotassium hydrogen phosphate, 0.10% of potassium chloride, 0.01% of magnesium sulfate, and 0.0008% of iron sulfate. 24h after cultivation, according to 12m 3 / h ventilation for aeration culture, after 24 hours of liquid culture, according to 15m 3 The aeration rate per hour was used for aeration culture, the temperature of the liquid culture process was controlled at 30°C, the light intensit...

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Abstract

The invention provides a fermentation production method for hypocrea virens. A hypocrea virens strain is activated and cultured in a primary seed liquid medium and a secondary liquid medium for expanding reproduction, strong thalli and a certain number of spores are obtained, a solid medium formed by a compounded carbon source and nitrogen source is inoculated with secondary seed liquid, the compounded carbon source and nitrogen source are put in fermentation production, and meanwhile most proper non-bio impact factors are provided; rapid growth and spore production of hypocrea virens are promoted, and the content of the spores generated after solid fermentation is 100,000,000,000 per g.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a fermentation production method of H. chlorogenes and a solid culture medium of H. chlorogenes. Background technique [0002] Hypocrea virens is a hyperparasitic bacterium that inhibits a variety of soil-borne plant pathogenic fungi. The colony grows rapidly, and after 4 days of cultivation, the diameter is 7-9.5 cm. The aerial hyphae are curly, white or gray. Conidiospores are abundant and nearly spread flat on the flat plate. As the main mechanism of biological control of plant diseases, H. chloroform has been paid more and more attention by plant pathologists in various countries. As an important factor of biological control, hyperparasites play an important role in the control of plant diseases. [0003] In the prior art, there are few reports about the fermentation process of H. chlorogenes. Only the invention patent of CN10375911A records relevant content ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 罗建辉石仕福李英武梁小文王根豪严艺波李肖宇吴俊杰石峥曾升华罗双燕
Owner JIANGXI TIANREN ECOLOGY
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