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Preparation method for producing sodium heparin by using small sheep intestines

A technology of heparin sodium and small intestine, which is applied in the field of preparation of isolated biochemical drugs, can solve the problems of unreasonable utilization of sheep small intestinal mucosa, failure to reach industrialized production, and environmental pollution by waste water, and achieve the solution of odor in workshops, reduction of production costs, The effect of high yield

Active Publication Date: 2010-12-15
山东绅联药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

China is a large animal husbandry country, and the annual supply of sheep small intestine is huge, but because the number of mast cells in sheep small intestine mucosa is much smaller than that in pig small intestine mucosa, 10,000-12,000 sheep are often needed to produce 100 million units of heparin sodium Small intestinal mucosa, and the yield of the prepared heparin sodium is low, the activity is not stable enough, the actual operation process is relatively strict and complicated, and it cannot meet the requirements of industrial production, so that the use of sheep small intestinal mucosa is unreasonable for a long time, and in production A large amount of waste water will be generated in the process to pollute the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation method of producing heparin sodium by utilizing sheep small intestine adopts the following steps:

[0024] (1) Preparation of intestinal mucosa:

[0025] ①Take 200 lamb small intestines and rinse them with water to remove residual feces, clean them, and freeze them for storage;

[0026] ②Defrost the small intestine of the sheep naturally at room temperature, pour clean water into the intestine, squeeze out the small intestinal mucosa with a casing machine, operate evenly, and the mucosa enters the mucosa pool along the mucosal pipeline;

[0027] ③Intestine head and broken intestine skin in the scraping process should be minced and then put into the mucous membrane pool, collecting a total of 120 kg of mucous membrane;

[0028] (2) Enzymolysis:

[0029] ① Turn on the mucous membrane pump, pump 120 kg of mucous membrane from the mucous membrane pool into the enzymolysis tank, add 216 kg of water into the enzymolysis tank, turn on the mixer to stir at a s...

Embodiment 2

[0051] The preparation method of producing heparin sodium by utilizing sheep small intestine adopts the following steps:

[0052] (1) Preparation of intestinal mucosa:

[0053] ① Take 100 sheep small intestines and wash them with water, remove the residual feces, clean them, and freeze them for storage;

[0054] ②Defrost the small intestine of the sheep naturally at room temperature, pour clean water into the intestine, squeeze out the small intestinal mucosa with a casing machine, operate evenly, and the mucosa enters the mucosa pool along the mucosal pipeline;

[0055] ③Intestine heads and broken intestine skins in the scraping process should be minced and put into the mucous membrane pool, and a total of 70 kg of mucous membranes should be collected;

[0056] (2) Enzymolysis:

[0057] ① Turn on the mucous membrane pump, pump 70 kg of mucous membrane from the mucous membrane pool into the enzymolysis tank, add 119 kg of water into the enzymolysis tank, turn on the mixer to s...

Embodiment 3

[0079] The preparation method of producing heparin sodium by utilizing sheep small intestine adopts the following steps:

[0080] (1) Preparation of intestinal mucosa:

[0081] ① Take 300 sheep small intestines and wash them with water to remove the residual feces, clean them, and freeze them for storage;

[0082] ②Defrost the small intestine of the sheep naturally at room temperature, pour clean water into the intestine, squeeze out the small intestinal mucosa with a casing machine, operate evenly, and the mucosa enters the mucosa pool along the mucosal pipeline;

[0083] ③Intestine heads and broken intestine skins in the scraping process should be minced before being put into the mucous membrane pool, and a total of 150 kg of mucous membranes should be collected;

[0084] (2) Enzymolysis:

[0085] ① Turn on the mucous membrane pump, pump 150 kg of mucous membrane from the mucous membrane pool into the enzymolysis tank, add 285 kg of water into the enzymolysis tank, turn on...

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Abstract

The invention discloses a preparation method for separating a biochemical medicament from small sheep intestine mucosas, in particular a preparation method for producing sodium heparin by using small sheep intestines. The preparation method for producing the sodium heparin by using the small sheep intestines is characterized by comprising the following steps of: preparation of intestine mucosas, enzymolysis, adsorption, elution, precipitation, desalting, dehydration and drying to obtain the sodium heparin. Therefore, the method has the advantages of high yield, low sodium heparin activity loss, stable quality and capability of solving the problem of stink in a sodium heparin workshop by adopting a workshop waste gas purification treatment device; 10,000 to 12,000 small sheep intestine mucosas are needed for producing 100 million units of sodium heparin by using the conventional process, and 6,000 to 8,000 small sheep intestine mucosas are needed for producing 100 million units of sodium heparin by using the preparation method of the invention; and the valence and activity of the sodium heparin are greatly improved so that the production cost is greatly reduced, and the profit is improved by over 40 percent.

Description

(1) Technical field [0001] The invention relates to a preparation method for isolating biochemical drugs from sheep small intestine mucosa, in particular to a preparation method for producing heparin sodium from sheep small intestine. (2) Background technology [0002] Heparin sodium is a mucopolysaccharide sulfate anticoagulant drug. It is the sodium salt of aminodextran sulfate extracted from the intestinal mucosa of pigs or cattle. It belongs to mucopolysaccharides and naturally exists in mast cells. It is widely used in the treatment of arterial and venous thrombosis and pulmonary embolism, ischemic stroke, unstable angina, acute myocardial infarction, etc. At present, heparin sodium is mainly extracted from the small intestinal mucosa of pigs and sheep, bovine lungs, and salted casing saline. Heparin sodium is a natural anticoagulant substance and is one of the main export drugs in my country. With the deepening of research, it has been found that heparin sodium not on...

Claims

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Application Information

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IPC IPC(8): C08B37/10C12S3/02
Inventor 刘琪甄爱华刘建洋
Owner 山东绅联药业股份有限公司
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