Application of polyaspartic acid as agricultural bacillus spray drying protective agent

A technology of polyaspartic acid and drying protective agent, which is applied in the fields of application, agriculture, bacteria, etc., can solve the problem of undiscovered polyaspartic acid, etc., and achieve the effect of increasing the yield of viable bacteria and reducing the loss of activity

Active Publication Date: 2012-10-03
SHENZHEN BATIAN ECOTYPIC ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, so far, no application report has been found to utilize polyaspartic acid as a protective agent for Bacillus and other microorganisms in agriculture.

Method used

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  • Application of polyaspartic acid as agricultural bacillus spray drying protective agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Ferment Bacillus megaterium in liquid and make it in the sporulation stage, add 5% (kg / L) light calcium carbonate and 5% (kg / L) sterilized poly Aspartic acid, as a comparison, only 5% (kg / L) of light calcium carbonate was added, stirred evenly and then spray-dried. The spray drying conditions are as follows: the air inlet temperature is 140°C, the air outlet temperature is 60°C, and the nozzle pressure is 150KPa.

Embodiment 2

[0024] Subtilis (Bacillus subtilis) was fermented in liquid to make it in the sporulation stage, and 5% (kg / L) of light calcium carbonate and 5% (kg / L) of sterilized poly Aspartic acid, as a comparison, only 5% (kg / L) of light calcium carbonate was added, stirred evenly and then spray-dried. The spray drying conditions are as follows: the air inlet temperature is 140°C, the air outlet temperature is 60°C, and the nozzle pressure is 150KPa.

Embodiment 3

[0026] Ferment Bacillus mucilaginosus in liquid and make it in the spore formation stage, add 5% (kg / L) light calcium carbonate and 5% (kg / L) sterilized The polyaspartic acid, contrast only adds the light calcium carbonate of 5% (kg / L), sprays drying after stirring evenly. The spray drying conditions are as follows: the air inlet temperature is 140°C, the air outlet temperature is 60°C, and the nozzle pressure is 150KPa.

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Abstract

The invention belongs to the production field of agricultural microbial inoculum, which particularly relates to an application of polyaspartic acid as agricultural bacillus spray drying protective agent and an application method for preparing agricultural bacillus microbial inoculum. After the liquid fermentation culture solution of agricultural bacillus is adsorbed by light calcium carbonate to regulate thallus concentration, the sterilized polyaspartic acid is added to serve as the protective agent to carry out spray drying; and the activity loss of the agricultural bacillus in the spray drying process can be reduced, the viable bacteria yield is improved, the conditioning action on soil by the polyaspartic acid as a fertilizer increasing ingredient is performed in the use process with the agricultural bacillus, and the application of the polyaspartic acid is expanded. The average viable bacteria yield of the agricultural agricultural bacillus microbial inoculum produced with the method disclosed by the invention is above 60%, and the viable bacteria exceed 1010 / g, thereby satisfying the production requirement of the compound microbial fertilizer or the microbial organic fertilizer.

Description

technical field [0001] The invention relates to the field of agricultural microorganism production, in particular to the use of polyaspartic acid as a spray-drying protective agent for agricultural bacillus and its application method in the preparation of agricultural bacillus inoculum. Background technique [0002] For nearly half a century, chemical fertilizers have played a vital role in agricultural production, but excessive reliance on chemical fertilizers has become a bottleneck for sustainable agricultural development. As resource and environmental problems become increasingly prominent, people pay more and more attention to ecological environment and food safety issues. As an important supplement to traditional chemical fertilizers, microbial fertilizers have been widely used in ecological agriculture. [0003] Bacillus for agricultural use (such as Bacillus megaterium, Bacillus colloid, Bacillus subtilis and Bacillus licheniformis, etc.) is an important strain for t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C05G3/00C05G3/04C12R1/11C12R1/125C12R1/07C05G3/80
Inventor 罗树生王宗抗唐枫邵海峰段继贤吴小丽
Owner SHENZHEN BATIAN ECOTYPIC ENG
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