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Method for concentrating, stabilizing and separating liquid enzyme

A liquid enzyme and stabilization technology, which is applied in the field of separation, concentration and stabilization of liquid enzyme preparations, and can solve the problems of enzyme inactivation and low content of encapsulated biological enzymes

Inactive Publication Date: 2014-04-23
SHANXI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] At present, some technologies mention the use of supramolecules to encapsulate liquid enzyme preparations. However, due to the characteristics of the three-dimensional structure of biological enzymes, in supramolecular technology, enzyme inactivation and low content of encapsulated biological enzymes have been encountered to varying degrees.

Method used

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  • Method for concentrating, stabilizing and separating liquid enzyme
  • Method for concentrating, stabilizing and separating liquid enzyme
  • Method for concentrating, stabilizing and separating liquid enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The nonionic surfactant EO 11 PO 69 EO 11 (HLB value 6.0) 0.9g was dissolved in 50g water, then 1g of liquid protease preparation was mixed with it, the mixture was added dropwise to 0.8g α-cyclodextrin, and then 2.4g 1,2-propanediol stabilizer was added and mixed well.

[0044]The above-mentioned uniformly mixed liquid was sealed and stirred at 20°C for 20 hours. The enzyme activity of the liquid protease preparation was determined according to the national standard GB / T23527-2009, as shown in the table below.

[0045]

[0046] Dynamic Light Scattering (DLS) was used to test the nanostructure of the sample. Particle size distribution of supramolecular microspheres (see figure 1 ), the average size of the encapsulated enzyme particles is about 270nm.

Embodiment 2

[0048] The nonionic surfactant EO 133 PO 50 EO 133 (HLB value 26.0) 9g was dissolved in 50g ethanol, then 38g liquid lipase preparation was mixed with it, the mixture was added dropwise to 1.5g β-cyclodextrin, and then 0.5g 1,3-propanediol stabilizer was added and mixed well.

[0049] The above-mentioned uniformly mixed liquid was sealed and stirred at 20°C for 22 hours. The enzyme activity of the liquid lipase preparation was determined according to the national standard GB / T23527-2009, as shown in the table below.

[0050]

[0051] Dynamic Light Scattering (DLS) was used to test the nanostructure of samples. Particle size distribution of supramolecular microspheres (see figure 2 ), the average size of the encapsulated enzyme particles is about 298nm.

Embodiment 3

[0053] The nonionic surfactant EO 3 PO 43 EO 3 (HLB value 6.0) 20g is dissolved in 100g of acetone, then take 18g of liquid hemicellulase preparation and mix with it, the mixture is added dropwise to 10g of small cyclopan, then add 0.55g of 1,2-butanediol stabilizer and mix well .

[0054] The above-mentioned uniformly mixed liquid was sealed and stirred at 30°C for 24 hours, and the enzyme activity of the liquid hemicellulase preparation was determined according to the national standard GB / T23527-2009, as shown in the table below.

[0055]

[0056] Dynamic Light Scattering (DLS) was used to test the nanostructure of samples. The particle size distribution of supramolecular microspheres is shown in the figure below (see image 3 ), the average size of the encapsulated enzyme particles is about 806nm.

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Abstract

The invention provides a method for concentrating, stabilizing and separating liquid enzyme. The liquid enzyme comprises the following components by weight percent: 1.0-20.0% of macrocyclic molecular synthetic receptor, 0.1-50% of nonionic surfactant, 0.25-5% of enzyme stabilizer, 0.01-80% of biological enzyme, and the balance of polar solvent. The method comprises the following steps: mixing the components, and reacting for 1-24 hours under the conditions that the pH is 3-12 and the temperature is 0-80 DEG C; forming supramolecular microspheres containing the biological enzyme in the solution, wherein the particle size of the supramolecular microspheres is 10-1000nm. The selected materials are friendly to the environment, harmless, and easy to degrade. Above all, the method does not damage the activity of enzyme, the enzyme has high activity within an extended period, and the activity of the enzyme coated in the supramolecular microspheres is improved by 20% in comparison with the activity of common liquid enzyme. The activity loss of the biological enzyme is smaller than 10% four weeks after the liquid enzyme is applied to the formula of a textile emulsifier or a liquid detergent.

Description

technical field [0001] The invention relates to a liquid enzyme preparation, in particular to methods for concentrating, stabilizing and separating liquid enzyme preparations. Background technique [0002] Liquid enzyme preparation is a raw material that is easy to use, environmentally friendly and has good biodegradability. Specific catalytic properties and mild reaction conditions have been more and more used and valued in the industry. However, the concentration, stabilization and separation of liquid enzyme preparations have always been a problem that plagues people. [0003] Of course, there are many people trying to find solutions to the problems of concentration, stabilization and separation of liquid enzyme preparations. And put forward some feasible technologies, including adding enzyme stabilizer, membrane separation technology, microwave vacuum concentration method, reagent concentration, and temperature-sensitive hydrogel method. [0004] The method of adding ...

Claims

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Application Information

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IPC IPC(8): C12N9/96
CPCC12N9/96
Inventor 张剑王素文徐淑宜
Owner SHANXI UNIV
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