37results about How to "Reduced purification process" patented technology

The invention provides a novel library construction method based on an illumina sequencing platform, and in particular provides a small-fragment DNA library construction method. Aiming at the defects of the small-fragment DNA library construction of the existing illumina sequencing platform, the purification steps can be reduced, the loss and waste of a library are reduced, the cost is lowered, and the working efficiency is improved.

The invention relates to an oredressing method for pertinently reducing content of sesquioxide of iron and aluminum (R2O3) in the collophanite floatation process in advance. The process comprises the following steps of: (1) levigating raw collophanite until grains with the grain size of 0.038mm are more than or equal to 76 weight percent, and controlling the pulp mixing concentration to be 25-40 weight percent; (2) introducing ore pulp into a mineralizing agitation tank of a floatation machine, adding an agent for floating conventional mid-low grade collophanite for pneumatic flotation, ensuring that a spindle speed of the floatation machine is 1,800-2,100r/min, the floating aeration quantity is 0.05-0.10m<3>/h and the floating scraper speed is 20-30r/min, and obtaining phosphate concentrate subjected to rough concentration; and (3) controlling the pH of the phosphate concentrate subjected to rough concentration to be 4.5-5.5, adding a collector for removing R2O3, performing pneumatic flotation for 4 to 8 minutes, removing an impurity of R2O3 from foam, and obtaining base flow, namely a high grade phosphate concentrate product. By the method, the R2O3 content in the concentrate can be obviously reduced, the quality of the phosphate concentrate is improved, and production processes, flows and cost during purification in the subsequent acid making process can be reduced.

The invention provides a levalbuterol intermediate and a levalbuterol hydrochloride synthesis method, and relates to a levalbuterol intermediate and a method for preparing levalbuterol hydrochloride from the intermediate. The method comprises steps as follows: 2-halogenate-1-(2,2-dimelthyl-4-hydrogen-benzo [d][1,3] dioxane)-butanone and organic amine have a Hoffman alkylation reaction to prepare a compound in the formula 2, the structural formula of the compound is shown in the specification, and the compound in the formula 2 is subjected to a reduction reaction, optically pure organic acid resolution and deprotection by hydrochloric acid to obtain levalbuterol hydrochloride. The method does not need processes of protection or deprotection and the like of hydroxyl groups on a benzene ring, protection, deprotection and purification processes are reduced, the synthesis route is short, operation is simple, meanwhile, borane-thioether does not need to be used as a reduction agent, and safety and environmental protection are realized.

The invention discloses a method and device for removing metal impurities in polycrystalline silicon by electron beam overheat smelting, and belongs to the field of metallurgy. The device comprises a water-cooled copper smelting crucible, wherein an inclined sidewall design is adopted for the water-cooled copper smelting crucible; the inner sidewall of the water-cooled copper smelting crucible forms an included angle of 105 to 120 degrees with the bottom of the water-cooled copper smelting crucible; a graphite bushing is arranged in the water-cooled copper smelting crucible; the outer surface of the graphite bushing is attached to the inner surface of the water-cooled copper smelting crucible to form a close fit design; the bottom of the graphite bushing and the bottom of the water-cooled copper smelting crucible are horizontal, the inner surface sidewall of the graphite bushing forms an included angle of 95 to 100 degrees with the bottom of the graphite bushing. According to the device, the metal impurities are removed by overheat smelting, so that the number of times of subsequent directional solidification and ingot casting can be reduced, a purification process can be eliminated, and the production cost can be reduced; by electron beam overheat smelting for polycrystalline silicon purification, post directional solidification can be reduced by one time or more, and the metal impurities in the polycrystalline silicon can be reduced by 30 percent or more.

The invention discloses a separation and purification method of recombinant parathyroid hormone (PTH), which comprises the following steps: 1) bacterium crushing and inclusion body treatment: carryingout high-pressure homogenization and bacterium crushing, carrying out high-speed centrifugation after bacterium crushing to collect precipitates, carrying out resuspension washing on the precipitates, and mixing and redissolving the precipitates with a redissolved solution; 2) affinity chromatography; wherein a filler is selected from one of Ni-NTA, GST Sepharose and MBP Sepharose; 3) carrying out enzyme digestion and renaturation on a column; 4) carrying out cation exchange chromatography and/or hydrophobic chromatography, and (5) desalting. According to the present invention, through the method, the PTH (1-34) polypeptide with biological activity can be efficiently extracted from an inclusion body so as to make the large-scale industrial production of the PTH (1-34) possible.

The invention discloses a production device and a production method of electronic-grade hydrofluoric acid. The production method comprises the following steps: gasifying industrial anhydrous hydrogenfluoride serving as a raw material through an evaporator, conveying the gasified anhydrous hydrogen fluoride to a purification tower, and carrying out rectifying to obtain high-purity hydrogen fluoride gas; conveying the high-purity hydrogen fluoride gas to an absorption tower, and conducting absorbing with a hydrofluoric acid liquid phase having a certain concentration to obtain a crude electronic-grade hydrofluoric acid product; and finally, carrying out ultra-pure filtration to obtain a finished electronic-grade hydrofluoric acid product. The technical problems of low yield and low purity in the prior art are solved in the invention, and the production method meets environmental protection requirements and is suitable for industrial large-scale production.

The invention relates to the field of water treatment, and particularly relates to a rainwater sewage treatment system. The rainwater sewage treatment system comprises a discharge pipe, a sewage pipe and a rainwater pipe, wherein a transfer station is arranged between the discharge pipe and the sewage pipe and the rainwater pipe; one end, far away from a road surface, of the discharge pipe is fixedly connected and communicates with the transfer station; one end of the sewage pipe is fixedly connected and communicated with the transfer station, and the other end of the sewage pipe communicates with a sewage treatment plant; one end of the rainwater pipe is fixedly connected and communicates with the transfer station, and the other end of the rainwater pipe communicates with a river channel; the rainwater pipe and the sewage pipe are not opened and closed at the same time; and a conversion device for converting the opening and closing states of the rainwater pipe and the sewage pipe is arranged between the rainwater pipe and the sewage pipe. According to the rainwater sewage treatment system, the opening states of the rainwater pipe and the sewage pipe are converted, sewage and rainwater are separated, the process of purifying the rainwater by the sewage treatment plant is reduced, and the effect of reducing the power consumption waste of the sewage treatment plant is achieved.

The invention discloses a preparation method of trans-4-(trans-4-alkyl cyclohexyl) cyclohexanecarboxaldehyde, and belongs to the technical field of liquid crystal intermediate synthesis methods. The preparation method comprises the following steps: by taking (4-alkyl cyclohexyl) benzene as an initial raw material, carrying out carbonylation reaction, aldehyde group protection hydrogenation and deprotection to obtain the trans-4-(trans-4-alkyl cyclohexyl) cyclohexanecarboxaldehyde. The preparation method of the compound is simple, and the purposes of shortening the route, simplifying post-treatment and saving working hours and cost are achieved.

The invention discloses a subunit influenza vaccine split agent and application thereof, and belongs to the technical field of vaccine production. The subunit influenza vaccine split agent comprises nonoxynol-9 with a mass concentration of 0.1-5%, sodium hydroxide with a mass concentration of 1-5 g/L, sodium dihydrogen phosphate with a mass concentration of 5-20 g/L, sucrose with a mass concentration of 10-60% and the balance distilled water. According to the subunit influenza vaccine split agent and application thereof, the split agent is subjected to a density gradient centrifugation process, due to the fact that the split agent with the formula has the character of efficiently splitting influenza viruses, virus particles do not need to be split in advance, and are split in the centrifugation process instead, that is to say, one-step splitting is achieved, thus a purification treatment process is shortened, no obvious M protein bands or N protein bands appear in a product PAGE electrophoresis, and the purity of a main antigen component HA of a vaccine is higher than 90%, and is higher than those of products which are obtained by adopting aforehand splitting and other split agentformulas.

The invention belongs to the field of alkaloid and discloses a method for synthesizing active alkaloid Misszrtlide and an analogue thereof. The method comprises the following steps: adopting chiral raw materials, namely L-methyl tryptophan hydrochloride and L-phenyl lactic acid as substrates and performing non-racemic amide connection under the condition of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/1-hydroxybenzotriazole; subsequently reacting with tert-butyldimethylsilyl chloride under imidazole conditions, so that hydroxyl is promoted, and the competition with N-H on tryptophan indole in the process of reaction with R1Br (R1 can be one of 3,3-dimethylpropenyl, p-toluoyl and decanoyl) under alkaline conditions in the next step is avoided; finally, mildly and efficientlyremoving the tert-butyldimethylsilyl protection with a hydrogen fluoride pyridine solution to obtain the Misszrtlide or the analogue thereof with high yield and easiness in processing through four-step reaction. The method disclosed by the invention is simple, low in raw material cost, mild in reaction conditions, high in purity and easy to industrialize and has wide market prospect.

A preparation method of gingko leaf wine comprises the following steps of (1) preparation of gingko leaf dry powder: picking green and fresh gingko leaves without being eaten by worms in summers and autumns, drying the green and fresh gingko leaves in the sun, pulverizing the dried gingko leaves, sieving the smashed gingko leaves by using a sieve with mesh of 30-70, and packing the sieved gingko leaves for subsequent use; (2) preparation of extract solvent: diluting wine or alcohol with water until the concentration of the wine or alcohol is 20-36%, adding 1% of activated carbon in the diluted wine or alcohol, performing deodorization treatment for 5-10 days and filtering for subsequent use; (3) preparation of gingko leaf leach liquor: adding 300-900g of gingko leaf dry powder in 100L of extract solvent, stirring the mixture at intervals of 2-3 days for four times to five times, standing for 48-60 hours, and sucking up supernatant liquor by siphoning; and (4) preparation of syrup: preparing 50-60% of liquor by using sugar and water, adding 0-1% of citric acid in the liquor, boiling the mixture, removing bubbles, filtering the hot mixture by using filtering cloth with mesh of 200 to remove residues, weighing 13-95% of gingko leaf leach liquor, 0-10% of syrup, 0-10% of fruit juice and the balanced water according to volume ratio, stirring and standing in a sealed environment for 20-30 days, and filtering, sterilizing and filling to obtain the gingko leaf wine.

The invention provides a method for purifying inosine from fermentation liquor, and relates to the technical field of chemical engineering. The method for purifying inosine from fermentation liquor comprises the following steps: S1, selecting inosine fermentation liquor for treatment; S2, putting the inosine fermentation liquor filtered in the step S1 into a reaction kettle; S3, separately storing the filtrate collected in S2; S4, concentrating, crystallizing and drying the inosine aqueous solution filtered in S2; S5, filtering the filtrate in the step S2 by using a micro-filtration membrane; S6, purifying the filtrate filtered in the step S5 and the wastewater collected in the step S3 by using a sewage treatment device. The inosine fermentation liquor filtrate glycogen is collected, meanwhile, purification and discharge are conducted in a filtrate sewage treatment mode, the environmental protection pressure is small, inosine can be collected to the maximum extent through the arrangement that the inosine fermentation liquor is diluted and filtered at the same time through the nanofiltration membrane, and the device is worthy of being vigorously popularized.