Method for purifying inosine from fermentation liquor

A technology for fermentation broth and inosine, applied in the field of purifying inosine, can solve the problems of high pressure on environmental protection of waste, low product recovery rate and high energy consumption, and achieve the effects of protecting the environment, high yield and reducing environmental pollution

Inactive Publication Date: 2021-07-30
JINAN MINGXIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a method for purifying inosine from fermentation broth, which solves the problems of a large amount of waste produced by the conventional process of purifying inosine, high pressure on environmental protection, long process, high energy consumption, and low product recovery rate.

Method used

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Embodiment 1

[0025] Embodiments of the present invention provide a method for purifying inosine from fermentation broth, comprising the following steps:

[0026] S1. Select the inosine fermentation broth for processing. When processing, add sodium hydroxide to the inosine fermentation broth to adjust the pH to 6. Adjusting the acidity can ensure that the inosine fermentation broth is easy to extract, and then use a stirring device to mix the inosine Stir the fermented liquid, heat it up to 110 degrees Celsius when stirring, and set the temperature to sterilize the inosine fermented liquid. After stirring for 2 minutes, stir to make the inosine fermented liquid mix evenly, so that the organic matter in the inosine fermented liquid Or separate impurities such as solid particles, use a filter device to filter the inosine fermentation broth, filter the solids and impurities in the inosine fermentation broth, and filter the solids or impurities in the inosine fermentation broth to ensure the nex...

Embodiment 2

[0038] Embodiments of the present invention provide a method for purifying inosine from fermentation broth, comprising the following steps:

[0039] S1. Select the inosine fermentation broth to process. When processing, add sodium hydroxide to the inosine fermentation broth to adjust its pH to 8. Then use a stirring device to stir the inosine fermentation broth. When stirring, it is heated to 115 degrees Celsius, after stirring for 3 minutes, use a filter device to filter the inosine fermentation broth, and filter the solids and impurities in the inosine fermentation broth;

[0040] S2. Put the inosine fermentation broth filtered in S1 into the reactor, then add tap water with the same volume as the inosine fermentation broth to the reactor, and then use the pump body to pass the diluted inosine fermentation broth through the nano Filter membrane, when the pump body is transporting the inosine fermentation broth, use another new pump body to slowly pass tap water into the inos...

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Abstract

The invention provides a method for purifying inosine from fermentation liquor, and relates to the technical field of chemical engineering. The method for purifying inosine from fermentation liquor comprises the following steps: S1, selecting inosine fermentation liquor for treatment; S2, putting the inosine fermentation liquor filtered in the step S1 into a reaction kettle; S3, separately storing the filtrate collected in S2; S4, concentrating, crystallizing and drying the inosine aqueous solution filtered in S2; S5, filtering the filtrate in the step S2 by using a micro-filtration membrane; S6, purifying the filtrate filtered in the step S5 and the wastewater collected in the step S3 by using a sewage treatment device. The inosine fermentation liquor filtrate glycogen is collected, meanwhile, purification and discharge are conducted in a filtrate sewage treatment mode, the environmental protection pressure is small, inosine can be collected to the maximum extent through the arrangement that the inosine fermentation liquor is diluted and filtered at the same time through the nanofiltration membrane, and the device is worthy of being vigorously popularized.

Description

technical field [0001] The invention relates to the technical field of chemical industry, in particular to a method for purifying inosine from fermentation broth. Background technique [0002] Inosine, also known as inosine, inosine nucleoside, etc., is a nucleoside compound formed by the combination of hypoxanthine and ribose. In the de novo synthesis of purine, inosine can be used to synthesize adenosine and guanosine The precursor of glycoside acid is suitable for leukopenia, thrombocytopenia, and various heart diseases caused by various reasons. Inosine can also be used as a coenzyme drug. Inosine is ATP, coenzyme A, ribonucleic acid and deoxygenation in the body A component of ribonucleic acid, it participates in the material metabolism and energy metabolism of the body. It can increase the level of ATP in the body, can be converted into a variety of nucleotides, and participate in protein synthesis. Inosine has good permeability to the cell membrane and can It directl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/167C07H1/06
CPCC07H19/167C07H1/06
Inventor 韩绍滋孔意隗浩
Owner JINAN MINGXIN PHARMA
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