Method for purifying inosine from fermentation liquor
A technology for fermentation broth and inosine, applied in the field of purifying inosine, can solve the problems of high pressure on environmental protection of waste, low product recovery rate and high energy consumption, and achieve the effects of protecting the environment, high yield and reducing environmental pollution
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Embodiment 1
[0025] Embodiments of the present invention provide a method for purifying inosine from fermentation broth, comprising the following steps:
[0026] S1. Select the inosine fermentation broth for processing. When processing, add sodium hydroxide to the inosine fermentation broth to adjust the pH to 6. Adjusting the acidity can ensure that the inosine fermentation broth is easy to extract, and then use a stirring device to mix the inosine Stir the fermented liquid, heat it up to 110 degrees Celsius when stirring, and set the temperature to sterilize the inosine fermented liquid. After stirring for 2 minutes, stir to make the inosine fermented liquid mix evenly, so that the organic matter in the inosine fermented liquid Or separate impurities such as solid particles, use a filter device to filter the inosine fermentation broth, filter the solids and impurities in the inosine fermentation broth, and filter the solids or impurities in the inosine fermentation broth to ensure the nex...
Embodiment 2
[0038] Embodiments of the present invention provide a method for purifying inosine from fermentation broth, comprising the following steps:
[0039] S1. Select the inosine fermentation broth to process. When processing, add sodium hydroxide to the inosine fermentation broth to adjust its pH to 8. Then use a stirring device to stir the inosine fermentation broth. When stirring, it is heated to 115 degrees Celsius, after stirring for 3 minutes, use a filter device to filter the inosine fermentation broth, and filter the solids and impurities in the inosine fermentation broth;
[0040] S2. Put the inosine fermentation broth filtered in S1 into the reactor, then add tap water with the same volume as the inosine fermentation broth to the reactor, and then use the pump body to pass the diluted inosine fermentation broth through the nano Filter membrane, when the pump body is transporting the inosine fermentation broth, use another new pump body to slowly pass tap water into the inos...
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