Protein-free, hydrolysate-free and serum-free culture medium and preparation method thereof

A serum-free medium and hydrolysate technology, applied in the field of serum-free medium without protein and hydrolysate and its preparation field, can solve the problems such as difficulty in controlling the differences between product batches, interference of research results, and difficulty in clarifying components, and achieve Small difference between product batches, simple ingredients, and the effect of reducing production costs

Active Publication Date: 2015-09-16
SICHUAN BAINUOJI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As a raw material for vaccine production, bovine serum has its obvious disadvantages: expensive, difficult to determine the composition, difficult to control product batch differences, and requires extremely strict testing procedures to ensure that the serum does not contain bacteria, viruses, etc. that may be carried by the serum-derived animal
At the same time, PK15 cells are also one of the models for cell metabolism research in vitro, and unclear components such as protein hydrolyzate and serum in the medium will interfere with the research results

Method used

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  • Protein-free, hydrolysate-free and serum-free culture medium and preparation method thereof
  • Protein-free, hydrolysate-free and serum-free culture medium and preparation method thereof
  • Protein-free, hydrolysate-free and serum-free culture medium and preparation method thereof

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Embodiment Construction

[0024] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. Unless otherwise indicated or defined, all terms used have their ordinary meanings in the art, which will be understood by those skilled in the art.

[0025] The preparation method of protein-free, hydrolyzate-free serum-free medium is as follows:

[0026] Add Tween 80 into a clean container with a capacity of 1000 ml, add 50 ml of water for injection to dissolve, add linoleic acid, linolenic acid, and arachidonic acid in sequence, wait until it is fully dissolved, rehydrate to 500 ml with water for injection, and then Add sodium chloride, glucose, and fructose in sequence, stir until clear, rehydrate with water for injection to 900 ml, and then add in sequence: calcium chloride, magnesium sulfate, potassium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, L fine hydrochloride Amino acid, L aspartic acid, L asparagine, L cyst...

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Abstract

The invention relates to a protein-free, hydrolysate-free and serum-free culture medium and a preparation method thereof. The culture medium contains the following substances: manganese gluconate, calcium citrate malate, disodium edetate, progesterone, alanyl-glutamine,glycerophosphate, fructose, vitamin C, vitamin E, sodium selenite, hydroxypropyl-beta-cyclodextrin, cholesterol, malic acid, oxaloacetic acid, vitamin B12, lipoic acid, ferrous sulfate, ferric citrate, uric acid, taurine, reduced glutathione, zinc sulfate, copper sulfate and ferric nitrate. The protein-free, hydrolysate-free and serum-free culture medium has the advantages that the cost is low, the difference among different batches of products is small, the purification step of down-stream products is simplified, and potential safety hazards are reduced.

Description

technical field [0001] The invention belongs to the field of cell engineering, in particular to a protein-free, hydrolyzate-free serum-free medium and a preparation method thereof, in particular to a protein-free, hydrolyzate-free serum-free medium suitable for the adherent culture of PK15 cells and its preparation method. Background technique [0002] Serum-free cell culture is the basis of cell metabolism research in vitro, and it is also one of the common technical means in the field of biological products and stem cell therapy. Most of the existing medium for serum-free culture of animal cells needs to add protein components such as insulin, transferrin, and albumin. Although these components have excellent cell growth-promoting effects, they are not compatible with protein-free cells. Compared with culture medium: its cost is high; the purification of downstream products is more complicated; and, the foreign virus that may be carried in it will bring serious safety ris...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
Inventor 扶星星吴福文王永胜
Owner SICHUAN BAINUOJI TECH CO LTD
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