Quick detecting method for aspartate amino transferase vitality

A technology of aspartic acid amino group and aspartic acid amino group, which is applied in the field of electrochemical detection, can solve the problems of high cleanliness, complicated steps, complicated process, etc., and achieves reduction of voltage, improvement of response speed and detection sensitivity, and accurate detection results. Effect
CN1782701AInactive Publication Date: 2006-06-07INST OF ELECTRONICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF ELECTRONICS CHINESE ACAD OF SCI
Publication Date
2006-06-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to electrochemical detection technology, and is quick detecting method of aspartate aminotransferase vitality. Electrochemical or optical change is produced corresponding to the vitality of the measured matter. The method includes: A. sucking the measured matter liquid drop into the reaction area between electric poles to generate oxidation-reduction on the surface of the electric poles so as to produce electron transfer and form current; and B. converting the aspartic acid into glutamic acid and oxaloacetic acid under the cooperated action of current and auxiliary factors and measuring the amount of glutamic acid and oxaloacetic acid so as to obtain the corresponding aspartate aminotransferase vitality. The present invention has raised response speed and detection sensitivity of current type enzyme sensor and lowered reaction voltage.
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Description

technical field

[0001] The invention relates to the technical field of electrochemical detection, and is a rapid test method for detecting the activity of aspartate aminotransferase (Aspartate Aminotransferase, AST), also known as aspartate aminotransferase (Glutamine-Oxalacetate Transferase, GOT). Redox reactions occur on the electrode surface. This paper mainly introduces the detection method of biosensor for aspartate aminotransferase (AST) and the preparation method of sensitive reagent, which can quickly detect the activity of aspartate aminotransferase (AST). technical background

[0002] In the just-concluded Athens Olympic Games, Chinese athletes worked hard and achieved good results that attracted the attention of the world. In addition to the hard work and sweat of the athletes, scientific training methods are also an important part of it. The evaluation of athletes' physical function status is the most critical part. A series of physiological and biochemical chan...

Claims

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