Quick detecting method for aspartate amino transferase vitality

A technology of aspartic acid amino group and aspartic acid amino group, which is applied in the field of electrochemical detection, can solve the problems of high cleanliness, complicated steps, complicated process, etc., and achieves reduction of voltage, improvement of response speed and detection sensitivity, and accurate detection results. Effect

Inactive Publication Date: 2006-06-07
INST OF ELECTRONICS CHINESE ACAD OF SCI
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Problems solved by technology

[0004] At present, there is no portable instrument product that uses electrochemical methods to detect aspartate aminotransferase (AST) on the market. Most of the detection equipment uses optical methods, which require high processing accuracy, high cleanliness, and portability. poor performance
Moreover, the traditional measurement method needs to draw a sufficient amount of blood from the forearm with a needle tube and separate the serum by centrifugation, which requires a large amount of blood, and the steps required are complicated and time-consuming, and the subjects feel a certai

Method used

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  • Quick detecting method for aspartate amino transferase vitality
  • Quick detecting method for aspartate amino transferase vitality
  • Quick detecting method for aspartate amino transferase vitality

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Embodiment

[0073] In this embodiment, ferricyanide is preferred as an electron carrier with redox effect, and the reaction that takes place is as follows:

[0074]

[0075] take as attached figure 1 The electrodes shown are spare.

[0076] Preparation of reagents: Take 0.1U / μl of glutamate dehydrogenase mother solution, dilute it to 16U / ml, take 50μl and add it to a centrifuge tube. Then take 4M aspartic acid solution, 0.16M α-ketoglutarate solution, 80mM trehalose solution, 40μM vitamin B6 (pyridoxal phosphate) solution, 2mM NAD+ solution, 0.8U / μl diaphorase solution, 0.2M iron Add 50 μl of each cyanate solution into the same centrifuge tube. Shake lightly until combined. 5 μl of the above solution was dropped onto the electrode reaction zone 3, and dried in a drying oven at 37° C. for 20 minutes. Take it out and add a cover layer 7 to form a capillary action channel.

[0077] On the prepared electrode, add 3~5μl standard solution through capillary action, and the following ...

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Abstract

The present invention relates to electrochemical detection technology, and is quick detecting method of aspartate aminotransferase vitality. Electrochemical or optical change is produced corresponding to the vitality of the measured matter. The method includes: A. sucking the measured matter liquid drop into the reaction area between electric poles to generate oxidation-reduction on the surface of the electric poles so as to produce electron transfer and form current; and B. converting the aspartic acid into glutamic acid and oxaloacetic acid under the cooperated action of current and auxiliary factors and measuring the amount of glutamic acid and oxaloacetic acid so as to obtain the corresponding aspartate aminotransferase vitality. The present invention has raised response speed and detection sensitivity of current type enzyme sensor and lowered reaction voltage.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection, and is a rapid test method for detecting the activity of aspartate aminotransferase (Aspartate Aminotransferase, AST), also known as aspartate aminotransferase (Glutamine-Oxalacetate Transferase, GOT). Redox reactions occur on the electrode surface. This paper mainly introduces the detection method of biosensor for aspartate aminotransferase (AST) and the preparation method of sensitive reagent, which can quickly detect the activity of aspartate aminotransferase (AST). technical background [0002] In the just-concluded Athens Olympic Games, Chinese athletes worked hard and achieved good results that attracted the attention of the world. In addition to the hard work and sweat of the athletes, scientific training methods are also an important part of it. The evaluation of athletes' physical function status is the most critical part. A series of physiological and biochemical chan...

Claims

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Application Information

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IPC IPC(8): G01N27/403G01N27/327
Inventor 郭宗慧蔡新霞刘春秀姜利英李华清罗贤波王慧周爱玉
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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