The invention relates to a waste bacterial stick biologically fermented feed and a preparation method thereof. The waste bacterial stick biologically fermented feed is prepared by taking waste bacterial sticks as main raw materials and adding corn flour, soybean meal, rice bran, vegetables, beet sugar residues, soybean probiotic preparations, hawthorn, purslane, acanthopanax senticosus, dandelion,plantains and the like for fermentation. According to the feed, abundant mycelia, proteins, amino acids, vitamins, minerals and other components contained in the waste bacterial sticks are fully utilized, and medicinal effects of hawthorn, purslane, acanthopanax senticosus, dandelion and plantains are exerted, so that appetite of livestock is increased, and disease resistance of livestock is improved. Finally, the fermented feed disclosed by the invention has the characteristics of rich nutrition, sour and fragrant taste, good palatability, rich bacterial proteins, high absorption conversionrate, high quality, no harm, low cost, improved breeding product quality and the like. Agricultural wastes such as the waste bacterial sticks are effectively utilized, so that development of circulating agriculture is facilitated, and breeding cost is reduced.