Preparation method of acanthopanax root extract

Abstract

The invention discloses a preparation method of an acanthopanax root extract, which comprises the following steps of: with acanthopanax root as a raw material, placing the material into excessive alcohol for soaking for at least 12h, then heating, extracting by reflux for 1-3h; repeating the operation for at least 2 times, mixing extraction solutions to obtain the acanthopanax root extract; and taking macroporous resin, packing by adopting a wet process, sampling the acanthopanax root extract to ensure that the acanthopanax root extract is saturated through absorption, washing until the Molishi reaction shows negative, and then carrying out alcohol gradient eluting, collecting eluents in all sections to obtain the acanthopanax root extract. The invention discloses a preparation method of the acanthopanax root extract. Experiments prove that when the concentration of a sample to be tested is 100 mu g / mL, a 50 percent alcohol elution part shows strongest NO release inhibition activity with inhibition rate being more than 95 percent; and when the concentration of the sample to be tested is 30mu g / mL, a 70 percent alcohol elution part shows strongest NO release inhibition activity with inhibition rate being more than 85 percent, and the 50 percent alcohol elution part and the 70 percent alcohol elution part have inhibition rates higher than the inhibition rate of positive control and have no cell toxicity.

Description

technical field [0001] The invention relates to a preparation method of Acanthopanax Radix Extract. Background technique [0002] Acanthopanax is the root, rhizome and stem of Araliaceae plant Acanthopanax senticosus (Rupr. et Maxim.) Harms. The stems, leaves and fruits can be used for medicinal purposes. Also known as Wujiashen and thorn crutches, it is mainly distributed in Northeast my country, North China, North Korea, Japan, Russia and other places. It has a long history of being widely used as a medicine in the motherland's medicine. It has the effects of replenishing qi, invigorating the spleen, invigorating the kidney and calming the nerves. It is used for spleen and kidney yang deficiency, physical weakness, loss of appetite, sore waist and knees, insomnia and dreaminess. [0003] One of the main components of Acanthopanax roots and rhizomes is phenolic glycosides and flavonoid glycosides, which are one of its biologically active components, and syringin and eleuth...

AI Technical Summary

Problems solved by technology

[0005] At present, the products related to Acanthopanax that are available in the market include Acanthopanax senticosus extract, Acanthopanax ginseng tea, Acanthopanax acanthopanica tablets, Acanthopanax senticosus Granules, Acanthopanax senticosus Injection, etc. The crude extract of the drug is processed, the composition is complex, and its active ingredient content and quality control are very vague, so it is difficult to conduct in-depth research

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