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Bacillus subtilis, bacterium agent and application thereof

A technology of Bacillus subtilis and microbial inoculum, applied in the field of agricultural microorganisms, can solve the problems of difficulty in disease resistance breeding, high cost, environmental pollution and the like

Active Publication Date: 2008-03-26
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the purposes of the present invention is to provide a kind of high-efficiency, broad-spectrum bactericidal activity that can be used for biological control against tomato gray mold disease-resistant breeding, and the problems of high cost, fruit pollution and environmental pollution caused by chemical pesticide control. Bacillus subtilis strains

Method used

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  • Bacillus subtilis, bacterium agent and application thereof
  • Bacillus subtilis, bacterium agent and application thereof
  • Bacillus subtilis, bacterium agent and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] The preparation of BAB-1 microbial inoculum is carried out according to the following steps:

[0044] (1) Strain activation: Activate the strain BAB-1 stored at -40°C on LB plate medium (30°C), pick a single colony and propagate it on LB slant medium (30°C) for later use. As an inoculum;

[0045] (2) Preparation of seed solution: Prepare LB liquid culture medium according to the conventional method, fill 100mL of LB culture solution in a 250mL Erlenmeyer flask, sterilize by autoclaving, moist heat, and after the temperature drops to room temperature, insert the steps in each bottle

[0046] (1) An inoculation loop of the above-mentioned activated BAB-1 strain, shake culture on a shaker at a rotation speed of 190 rpm, and incubate at 30°C for 24 hours to obtain the seed solution;

[0047] (3) Preparation of sucrose bean cake medium: according to 3.0% sucrose, 2% bean cake powder, 0.1% NaCl, 0.3% CaCO 3 , 0.02% KH 2 PO 4 And 0.03% MgSO 4 ·7H 2 O to prepare the medium, then ad...

Embodiment 2

[0051] The experiment was carried out in the greenhouse of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences, and Botrytis cinerea was selected (provided by the Plant Disease Biocontrol Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences). Seedlings are grown in flower pots, each with one seedling. Choose leaves with the same age and size. First, rinse the leaf surface with sterile water. After the sterile filter paper absorbs the water, the water is diluted with the water obtained in Example 1 (suspension Contains bacteria 10 8 cfu / mL) Bacillus subtilis BAB-1 inoculum, so that the surface of the leaf is fully coated, and then placed in a petri dish covered with double sterile filter paper, and at the same time connected to a tomato gray mold plate (diameter 6mm ), moisturizing culture. The leaves not treated with biocontrol bacteria were used as blank control. After the blank control is fully onse...

Embodiment 3

[0055] The experiment was carried out in the tomato greenhouse of Liu Yuhu, a farmer in Xushui County, Baoding City, Hebei Province, with two rows per plot, four replicates, and random block arrangement. During the experiment, the gray mold disease occurred slightly on the fruits in the whole shed, and the diseased fruits were removed before application. A knapsack-type hand sprayer was used to inoculate the Bacillus subtilis BAB-1 inoculum prepared in Example 1 (diluted with water, containing 10 8 cfu / mL); another control agent (50% Limexang wettable powder, provided by Baoding Kelvfeng Biochemical Technology Co., Ltd., Hebei Province, 600 times water dilution spray treatment) and blank control (clean water spray). After the blank control is fully onset, investigate the number of diseased fruits and healthy fruits per plant in each treatment plot, and calculate the incidence and control effect. The test results (see Table 3) show that the disease index of tomato gray mold after t...

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Abstract

The present invention discloses one kind of Bacillius subtilis BAB-1 preserved in the preservation number of CGMCC No. 2099, and the microbial preparation prepared with Bacillius subtilis BAB-1 and its preparation process. The Bacillius subtilis BAB-1 may be applied in controlling gray mold of tomato, and has the features of high efficiency, broad bacteriostasis spectrum, simple use, low cost, no environmental pollution, etc.

Description

Technical field [0001] The invention belongs to the field of agricultural microorganisms, and specifically relates to a Bacillus subtilis, and a microbial inoculum produced by the Bacillus subtilis, a preparation method and an application. Background technique [0002] Botrytis cinerea is a worldwide important disease of tomato, and its pathogen belongs to the genus Botrytis cinerea (Botrytis cinerea). The pathogen mainly damages fruits and poses a great threat to tomato production in protected areas (Li Baoju. Research Progress in Tomato Diseases and Pests and Their Control in China. China Agricultural Press, 1998.31-35). It began to spread in my country in the 1980s, and it is happening everywhere at present, which has become a restrictive obstacle to tomato cultivation. As there is no source of resistance to Botrytis cinerea, it is difficult to breed for disease resistance. Cultivation and control (controlling the temperature and humidity of the greenhouse) are used abroad (Mo...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02C12R1/125
Inventor 马平李社增鹿秀云郭庆港
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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