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Preparation method and application of sea cucumber epiphytic Bacillus subtilis exopolysaccharide

The technology of Bacillus subtilis and exopolysaccharide is applied in the field of preparation of Bacillus subtilis exopolysaccharide co-adhering to sea cucumbers, which can solve the problem that metabolites are not fully utilized, and achieves inhibiting the formation of micronucleus in broad bean root tip cells, Low cost and simple operation

Inactive Publication Date: 2015-06-10
DALIAN OCEAN UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, there are a large number of bacteria living in sea cucumbers and intestines. These bacterial metabolites have unique structures and functions, but these metabolites have not been fully utilized so far.

Method used

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  • Preparation method and application of sea cucumber epiphytic Bacillus subtilis exopolysaccharide
  • Preparation method and application of sea cucumber epiphytic Bacillus subtilis exopolysaccharide

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Embodiment Construction

[0015] Specific embodiments of the present invention will be described below.

[0016] A preparation method of sea cucumber co-associated Bacillus subtilis exopolysaccharide is characterized in that the method is carried out according to the following steps:

[0017] First configure the medium solution. Mix starch, potassium nitrate, NaCl, K2HPO4, MgSO4, FeSO4 into water to form a medium solution, and the mass percentage of each component in the medium solution is starch: 1.5%, potassium nitrate: 2%, NaCl: 0.05%, K2HPO4: 0.05%, MgSO4: 0.05%, FeSO4: 0.001%, and adjust the pH value of the medium solution to 5;

[0018] Then take the sea cucumber co-associated Bacillus subtilis HS22 (Bacillus subtilis HS22), which has been deposited in the General Microbiology Center (CGMCC) of the China Microbial Culture Collection and Management Committee on January 10, 2013, with the preservation number: CGMCC No: 7080; Place it in the culture medium solution, the ratio of HS22 to the culture...

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Abstract

The invention discloses a preparation method of a sea cucumber epiphytic Bacillus subtilis exopolysaccharide. The method has the characteristics of simple operation and low cost, and can stably acquire the exopolysaccharide of sea cucumber epiphytic Bacillus subtilis. At the same time, the sea cucumber epiphytic Bacillus subtilis exopolysaccharide prepared by the method has strong antioxidant activity, can inhibit the formation of Vicia faba root tip cell micronucleuses, and can be widely used in the anti-tumor medicines, biological control, food preservation, corrosion prevention, cosmetic additives and other fields, thus having broad market prospects and industrial value.

Description

Technical field [0001] The present invention relates to a method for preparing extracellular polysaccharide of sea cucumber co-associated with Bacillus subtilis, and the application of the polysaccharide in growth inhibition of bacteria and plant pathogens, removal of peroxide, anti-tumor and inhibition of micronucleus formation. Background technique [0002] Sea Cucumber is a phylum Echinoderm ( Echinodermata) , Sea cucumbers ( Holothuroidea) . There are about 1,200 kinds of sea cucumbers in the world, mainly living in tropical and temperate oceans. Common edible sea cucumbers include sea cucumbers, plum blossoms, and white melons. In addition to their edible value, these sea cucumbers also contain rich biological active substances, such as Sea cucumber toxins, sea cucumber polysaccharides, sea cucumber saponins, fatty acids, polypeptides, sea cucumber gangliosides, etc., these components have a variety of biological activities. In addition, a large number of bacteria live in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/00A61K31/715A61P35/00C12R1/125
Inventor 张付云刘晔王维才赵宇李振
Owner DALIAN OCEAN UNIV
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