Bacillus subtilis and application in preventing fusarium graminearum

A technology of Bacillus subtilis and Fusarium graminearum, which is applied in the directions of application, bacteria, fungicides, etc., can solve the problems of increasing difficulty in control and lack of ideal high-resistant wheat varieties, and achieve the effect of good application prospects.

Active Publication Date: 2014-01-08
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For many years, the control of wheat head blight has mainly relied on chemical pesticides, but with the environmental problems caused by chemical pesticides, coupled with years of drug use has led to the emergence of pathogenic bacteria resistant Fusarium graminea

Method used

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  • Bacillus subtilis and application in preventing fusarium graminearum
  • Bacillus subtilis and application in preventing fusarium graminearum
  • Bacillus subtilis and application in preventing fusarium graminearum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the separation and identification of bacteria

[0031] 1. Isolation of bacteria

[0032] (1) In October 2011, in the ultra-clean workbench, the loofah anthers collected in the suburbs of Beijing were placed in sterile distilled water and shaken for 15 minutes to prepare the bacterial suspension, and the shaker speed was 180rpm.

[0033] (2) Dilute the bacterial suspension in a concentration gradient with sterile distilled water, spread it on the NA medium plate, and incubate at 30°C for 24 hours. The colonies cover the entire plate. Use an inoculation loop to pick out the shape, size, The strains with different colors and transparency were streaked and purified on the plate, and the purified strains were applied to the confrontation culture experiment of Fusarium graminearum. The obtained strain with the strongest antagonistic ability against Fusarium graminearum was named SG6.

[0034] 2. Identification

[0035] (1) According to the method described in...

Embodiment 2

[0046] Embodiment 2, the antagonism of Bacillus subtilis SG6 to Fusarium graminearum

[0047] The antagonism of Bacillus subtilis SG6 to Fusarium graminearum was detected by confrontation culture method.

[0048]1. Use a puncher with a diameter of 5 mm to punch out the plate at the edge of the cultured pathogenic fungus Fusarium graminearum (Fusarium graminearum) FG1 colony, and use a sterilized toothpick to pick the plate into the center of the PDA plate.

[0049] 2. Inoculate the same volume and concentration of SG6 on a four-dot dash line at a distance of 25 mm from the center of the PDA plate. Take the PDA plate inoculated only with the plate of Fusarium graminearum as the control group, culture at 28°C for 4-5 days, and observe whether there is any antibacterial effect. bands formed. Do three parallels for each group. The result is as figure 1 and shown in Table 1.

[0050] figure 1 Middle, 1: control group; 2: SG6 group

[0051] figure 1 It shows that SG6 has a st...

Embodiment 3

[0055] Embodiment 3, SG6 are to the field control of Fusarium graminearum

[0056] 1. Culture SG6 overnight at 28°C in NB medium with shaking, and dilute to a concentration of 2.4×10 8 cfu / ml. The Shixin 828 variety was sprayed at the flowering stage, and the spray volume was 300ml, which was used as the experimental group.

[0057] At the same time, the 1000-fold dilution of 50% carbendazim and the NB dilution without inoculation were sprayed at the flowering stage of wheat Shixin 828, and the spray volume was 300ml, and the NB dilution without inoculation was used as the control group .

[0058] 2. The Fusarium graminearum FG 1 Use mung bean soup medium for shaking culture (28°C, 5 days) to obtain a bacterial suspension with a concentration of 2×10 5 cfu / ml.

[0059] Three, after finishing above-mentioned three groups of spraying treatments, carry out Fusarium graminearum suspension spray inoculation immediately, spray volume is 300ml, evenly spray on the plot of every ...

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Abstract

The invention discloses bacillus subtilis and an application in preventing fusarium graminearum. The preservation number of the bacillus subtilis disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.7621. As a biological prevention material against wheat scab, the bacillus subtilis has a good application prospect in developing a new biological pesticide or biological control bacterial agent.

Description

technical field [0001] The invention relates to a bacillus subtilis and its application in controlling Fusarium graminearum. Background technique [0002] Fusarium graminearum (Fusarium graminearum) is one of the important pathogens of gramineous crops, which can cause wheat head blight (Fusarium Head Blight, FHB). Fusarium graminearum is a major factor limiting wheat yield (Dubin, 1997) . my country's climatic conditions are very favorable for the occurrence of wheat head blight, and the perennial disease in the middle and lower reaches of the Yangtze River caused a yield loss of 10%-15%, and a production reduction of nearly 50% in a pandemic year (Huang Chang et al., 2000). Due to the influence of global warming and changes in farming systems such as straw returning to the field and no-tillage cultivation, the occurrence area of ​​wheat head blight in my country has rapidly expanded from the Yangtze River Basin to Northwest and North China (Zhang Hao, 2011), especially in...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
Inventor 刘阳赵月菊邢福国周露王龑
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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