Bacillus subtilis dcy-1 and application thereof in biofermentation
A Bacillus subtilis, bio-fermentation technology, applied in the field of agricultural microorganisms and animal husbandry, can solve the problems that have not been seen in the screening of Bacillus subtilis
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[0018] 1. Isolation and cultivation of microorganisms
[0019] 1.1 Sampling: Weigh 5g of pig manure compost samples (with a pore size of 1mm, that is, No. 18 sieve) and place them in a 50mL beaker, add glass beads, add 25mL of distilled water with a measuring cylinder, and place on a shaking table for 20 minutes to fully disperse the samples. And disperse the microbial cells and let it stand still. Then boil it with boiling water for about 15 minutes to kill other microorganisms and make the bacillus produce spores to obtain a diluted bacterial solution containing the bacillus.
[0020] 1.2 Preparation of diluted bacterial solution: use a sterile straw to absorb step (1) 10 -1 Transfer 1mL of the diluted bacterial solution into a test tube filled with 9mL of sterile water, blow and inhale 3 times, let the diluted bacterial solution fully mix, and serve as 10 -2 Dilute the bacterial solution; then use a sterile straw to draw 10 -2 Transfer 1mL of the diluent into a test tube...
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