Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of bacillus subtilis and production method for strains

A technology of Bacillus subtilis and a production method, applied in the field of biocontrol strains, can solve the problems of decreased disease prevention effect, outbreaks of pests, excessive pesticide residues and the like

Inactive Publication Date: 2009-12-16
SHIHEZI UNIVERSITY
View PDF2 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the past work in Xinjiang was mostly limited to follow-up, and basic research was still relatively weak, which led to insufficient innovation ability and research and development stamina. large gap
Therefore, in the agricultural production process in Xinjiang, chemical pesticides are still in an irreplaceable position, and the abuse of chemical pesticides leads to resistance of pathogenic bacteria, which leads to a decline in disease prevention effects, excessive pesticide residues in agricultural products, and outbreaks of other pests caused by irrational use of pesticides Disasters happen frequently

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation of fermentation medium: Take 10Kg of corn flour, 35Kg of soybean meal, 3Kg of honey, 1Kg of sodium carbonate, 1Kg of dipotassium hydrogen phosphate, 0.3Kg of magnesium sulfate, 1Kg of calcium carbonate, 0.1Kg of manganese sulfate, 0.1Kg of ferric chloride, and 0.5 kg of sodium chloride Kg, glycine 1Kg.

[0034]The ratio of the above raw materials and 1000L water is formulated into an emulsion, and the inoculation amount of 5% of the volume of the emulsion is inserted into the Bacillus subtilis strain and then placed in a fermenter, cultured and fermented at a temperature of 28 ° C and stirred at a speed of 180r / min, cultured for 50h.

Embodiment 2

[0036] Preparation of fermentation medium: corn flour 25Kg, soybean meal 15Kg, honey 5Kg, sodium carbonate 1.2Kg, dipotassium hydrogen phosphate 1.5Kg, glycine 1.5Kg, calcium carbonate 1Kg, magnesium sulfate 0.2Kg, manganese sulfate 0.15Kg, ferric chloride 0.1Kg , sodium chloride 0.5Kg;

[0037] The ratio of the above raw materials and 1000L water is formulated into an emulsion, and the inoculation amount of 8% of the volume of the emulsion is inoculated with Bacillus subtilis strains, and then placed in a fermenter, and cultured and fermented at a temperature of 30° C. for 60 hours.

Embodiment 3

[0039] Preparation of fermentation medium: corn flour 15Kg, soybean meal 25Kg, honey 4Kg, sodium carbonate 1.5Kg, dipotassium hydrogen phosphate 1.8Kg, glycine 2Kg, calcium carbonate 0.8Kg, magnesium sulfate 0.25Kg, manganese sulfate 0.15Kg, ferric chloride 0.15Kg , Sodium chloride 0.6Kg.

[0040] The ratio of the above raw materials and 1000L water is made into an emulsion, and the inoculation amount of 6% of the volume of the emulsion is inserted into the Bacillus subtilis strain and placed in a fermenter, and cultured and fermented at a temperature of 27°C and stirred at a speed of 210r / min, cultured for 55h.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of bacillus subtilis and a production method for strains. The production method comprises the following steps: mixing 10 to 25 grams of corn flour, 10 to 35 grams of bean pulp, 3 to 5 grams of honey, 1 to 2 grams of sodium carbonate, 1 to 2 grams of dipotassium phosphate, 1 to 2 grams of glycine, 0.5 to 1.5 grams of calcium carbonate, 0.2 to 0.3 gram of magnesium sulfate, 0.05 to 0.15 gram of manganese sulfate, 0.05 to 0.15 gram of ferric chloride, 0.3 to 0.8 gram of sodium chloride and 1,000 milliliters of water to form emulsion, inoculating 5 to 8 percent of bacillus subtilis strains by volume of the emulsion to the emulsion, then placing the emulsion into a fermentation tank, and culturing the bacillus subtilis strains for 45 to 60 hours at a temperature of between 28 and 30 DEG C to obtain the bacillus subtilis. The invention provides a biologically-prevented strain which is suitable for special geographic-climate conditions of Xinjiang and particularly can well control soil-borne diseases of economic crops mainly comprising tomatoes and cottons for processing, provides a process method suitable for industrialized mass production, and provides base conditions for developing new biological pesticide suitable for the special geographic-climate conditions of Xinjiang.

Description

Technical field: [0001] The present invention relates to the application of a kind of biocontrol bacterial strain that controls the occurrence of soil-borne diseases of economic crops based on processing tomato and cotton and the process method suitable for industrial scale production of the biocontrol bacterial strain, especially a kind of Bacillus subtilis ( Bacillus subtilis) and the production method of the bacterial strain. Background technique: [0002] At present, Xinjiang's unique geographical and climatic conditions are very suitable for cotton and processed tomato planting. The cotton planting and processing industry is an important pillar of Xinjiang's economic development. In addition, the processed tomato produced in Xinjiang has high content of red pigment and oryzanol, less mold, and good viscosity. It is the first-class raw material for processed tomato products and has become the most important planting and processing base for processed tomato. At the same ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N63/02A01P3/00C12N1/20C12R1/125
Inventor 赵思峰李春阎豫君吴艳李俊华
Owner SHIHEZI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products