Application of bacillus subtilis and production method for strains
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A technology of Bacillus subtilis and a production method, applied in the field of biocontrol strains, can solve the problems of decreased disease prevention effect, outbreaks of pests, excessive pesticide residues and the like
Inactive Publication Date: 2009-12-16
SHIHEZI UNIVERSITY
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Problems solved by technology
However, the past work in Xinjiang was mostly limited to follow-up, and basic research was still relatively weak, which led to insufficient innovation ability and research and development stamina. large gap
Therefore, in the agricultural production process in Xinjiang, chemical pesticides are still in an ...
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Embodiment 1
[0033] Preparation of fermentation medium: Take 10Kg of corn flour, 35Kg of soybean meal, 3Kg of honey, 1Kg of sodium carbonate, 1Kg of dipotassium hydrogen phosphate, 0.3Kg of magnesium sulfate, 1Kg of calcium carbonate, 0.1Kg of manganese sulfate, 0.1Kg of ferric chloride, and 0.5 kg of sodium chloride Kg, glycine 1Kg.
[0034]The ratio of the above raw materials and 1000L water is formulated into an emulsion, and the inoculation amount of 5% of the volume of the emulsion is inserted into the Bacillus subtilis strain and then placed in a fermenter, cultured and fermented at a temperature of 28 ° C and stirred at a speed of 180r / min, cultured for 50h.
[0037] The ratio of the above raw materials and 1000L water is formulated into an emulsion, and the inoculation amount of 8% of the volume of the emulsion is inoculated with Bacillus subtilis strains, and then placed in a fermenter, and cultured and fermented at a temperature of 30° C. for 60 hours.
[0040] The ratio of the above raw materials and 1000L water is made into an emulsion, and the inoculation amount of 6% of the volume of the emulsion is inserted into the Bacillus subtilis strain and placed in a fermenter, and cultured and fermented at a temperature of 27°C and stirred at a speed of 210r / min, cultured for 55h.
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Abstract
The invention discloses application of bacillus subtilis and a production method for strains. The production method comprises the following steps: mixing 10 to 25 grams of corn flour, 10 to 35 grams of bean pulp, 3 to 5 grams of honey, 1 to 2 grams of sodium carbonate, 1 to 2 grams of dipotassium phosphate, 1 to 2 grams of glycine, 0.5 to 1.5 grams of calcium carbonate, 0.2 to 0.3 gram of magnesium sulfate, 0.05 to 0.15 gram of manganese sulfate, 0.05 to 0.15 gram of ferric chloride, 0.3 to 0.8 gram of sodium chloride and 1,000 milliliters of water to form emulsion, inoculating 5 to 8 percent of bacillus subtilis strains by volume of the emulsion to the emulsion, then placing the emulsion into a fermentation tank, and culturing the bacillus subtilis strains for 45 to 60 hours at a temperature of between 28 and 30 DEG C to obtain the bacillus subtilis. The invention provides a biologically-prevented strain which is suitable for special geographic-climate conditions of Xinjiang and particularly can well control soil-borne diseases of economic crops mainly comprising tomatoes and cottons for processing, provides a process method suitable for industrialized mass production, and provides base conditions for developing new biological pesticide suitable for the special geographic-climate conditions of Xinjiang.
Description
Technical field: [0001] The present invention relates to the application of a kind of biocontrol bacterial strain that controls the occurrence of soil-borne diseases of economic crops based on processing tomato and cotton and the process method suitable for industrial scale production of the biocontrol bacterial strain, especially a kind of Bacillus subtilis ( Bacillus subtilis) and the production method of the bacterial strain. Background technique: [0002] At present, Xinjiang's unique geographical and climatic conditions are very suitable for cotton and processed tomato planting. The cotton planting and processing industry is an important pillar of Xinjiang's economic development. In addition, the processed tomato produced in Xinjiang has high content of red pigment and oryzanol, less mold, and good viscosity. It is the first-class raw material for processed tomato products and has become the most important planting and processing base for processed tomato. At the same ...
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