Application of bacillus subtilis and production method for strains
A technology of Bacillus subtilis and a production method, applied in the field of biocontrol strains, can solve the problems of decreased disease prevention effect, outbreaks of pests, excessive pesticide residues and the like
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Embodiment 1
[0033] Preparation of fermentation medium: Take 10Kg of corn flour, 35Kg of soybean meal, 3Kg of honey, 1Kg of sodium carbonate, 1Kg of dipotassium hydrogen phosphate, 0.3Kg of magnesium sulfate, 1Kg of calcium carbonate, 0.1Kg of manganese sulfate, 0.1Kg of ferric chloride, and 0.5 kg of sodium chloride Kg, glycine 1Kg.
[0034]The ratio of the above raw materials and 1000L water is formulated into an emulsion, and the inoculation amount of 5% of the volume of the emulsion is inserted into the Bacillus subtilis strain and then placed in a fermenter, cultured and fermented at a temperature of 28 ° C and stirred at a speed of 180r / min, cultured for 50h.
Embodiment 2
[0036] Preparation of fermentation medium: corn flour 25Kg, soybean meal 15Kg, honey 5Kg, sodium carbonate 1.2Kg, dipotassium hydrogen phosphate 1.5Kg, glycine 1.5Kg, calcium carbonate 1Kg, magnesium sulfate 0.2Kg, manganese sulfate 0.15Kg, ferric chloride 0.1Kg , sodium chloride 0.5Kg;
[0037] The ratio of the above raw materials and 1000L water is formulated into an emulsion, and the inoculation amount of 8% of the volume of the emulsion is inoculated with Bacillus subtilis strains, and then placed in a fermenter, and cultured and fermented at a temperature of 30° C. for 60 hours.
Embodiment 3
[0039] Preparation of fermentation medium: corn flour 15Kg, soybean meal 25Kg, honey 4Kg, sodium carbonate 1.5Kg, dipotassium hydrogen phosphate 1.8Kg, glycine 2Kg, calcium carbonate 0.8Kg, magnesium sulfate 0.25Kg, manganese sulfate 0.15Kg, ferric chloride 0.15Kg , Sodium chloride 0.6Kg.
[0040] The ratio of the above raw materials and 1000L water is made into an emulsion, and the inoculation amount of 6% of the volume of the emulsion is inserted into the Bacillus subtilis strain and placed in a fermenter, and cultured and fermented at a temperature of 27°C and stirred at a speed of 210r / min, cultured for 55h.
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