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Compound microbial flocculant and preparation method and application of compound microbial flocculant

A technology of composite microorganisms and flocculants, which is applied in the preparation of composite microbial flocculants and in the field of composite microbial flocculants, can solve problems such as difficult strain screening, unclear flocculation mechanism, secondary pollution, etc., to reduce sludge production and improve Survival rate, efficiency improvement effect

Inactive Publication Date: 2013-04-03
无锡格莱生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2012, the "polymeric aluminum-modified composite biological flocculant inorganic-organic composite flocculant and its preparation method" (application number 201210123810.7) was applied for by the invention patent of Shandong University. Allyl ammonium chloride in the presence of potassium persulfate and sodium sulfite initiator graft copolymerization prepared by the modified composite bioflocculant and polyaluminum (AlCl 3 ·6H 2 Dissolve the O raw material in distilled water, add Na 2 CO 3 Adjust the degree of alkalization, prepared by normal temperature reaction) mixed reaction in proportion, make full use of the characteristics of aluminum chloride, acrylamide and biological flocculant, but there will be residual aluminum ions and acrylamide in the sewage, forming secondary pollution
[0005] At present, the research on microbial flocculants has been mainly focused on the screening of flocculant-producing bacteria and the laboratory stage of strain culture solution treatment of wastewater, and most of them are aimed at a single bacterial group or extract organic macromolecules as flocculants through various processes. Difficult screening of bacterial strains, high cost, complex operation procedures, unclear flocculation mechanism, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Step 1: Carry out amplified culture separately

[0042] After the Bacillus jelly-like bacillus CGMCC1.231 is activated on the slant, it is inoculated in the liquid medium and cultured at 30°C for 120 hours. The liquid medium formula is 0.5% sucrose, 0.05% magnesium sulfate, 0.01% calcium carbonate, and 0.2 sodium hydrogen phosphate. %, ferric chloride 0.0005%, potassium feldspar 0.05% and the rest is water, pH is 7.0-7.3.

[0043] After Bacillus subtilis CICC23585 is activated on a slant, it is inoculated in a liquid medium and cultured at 30°C for 48 hours. The liquid medium formula is 1.5% soybean powder, 1% starch, 0.2% yeast extract, 1% glucose and the rest is water. The pH is 6.5.

[0044] Saccharomyces cerevisiae GIM2.39 was activated on a slant, inoculated in a liquid medium, and cultured at 30°C for 48 hours. The liquid medium was formulated with glucose 3%, beef extract 3%, tryptone 1.5%, peptone 2%, sodium acetate 0.5% %, 0.2% ammonium citrate, 0.058% magnes...

Embodiment 2

[0052] Step 1: Carry out amplified culture separately

[0053] The jelly-like bacillus CGMCC1.231, CGMCC1.232 and CICC23640 were activated on a slant, inoculated in a liquid medium, and cultured at 28° C. for 96 hours. The formulation of the liquid medium was the same as in Example 1.

[0054] After the Bacillus subtilis CGMCC1.1630 was activated on the slant, it was inoculated in a liquid medium, and cultured at 35° C. for 72 hours. The formulation of the liquid medium was the same as in Example 1.

[0055] Saccharomyces cerevisiae GIM2.39 was activated on a slant, inoculated in a liquid medium, and cultured at 30° C. for 48 hours. The formulation of the liquid medium was the same as in Example 1.

[0056] After the Penicillium heterophylla CGMCC3.5724 and ATCC52210 were activated on the slant, they were inoculated in the liquid medium and cultured at 30° C. for 72 hours. The formulation of the liquid medium was the same as in Example 1.

[0057] Viable bacteria count, the n...

Embodiment 3

[0063] Step 1: Carry out amplified culture separately

[0064] The jelly-like bacillus CGMCC1.231 and CGMCC1.232 were activated on a slant, inoculated in a liquid medium, and cultured at 28° C. for 108 hours. The liquid medium formulation was the same as in Example 1.

[0065] After the Bacillus subtilis CGMCC1.1630 was activated on the slant, it was inoculated in a liquid medium, and cultured at 35° C. for 72 hours. The formulation of the liquid medium was the same as in Example 1.

[0066] Saccharomyces cerevisiae GIM2.39 and CGMCC2.599 were activated on a slant, inoculated in a liquid medium, and cultured at 30° C. for 72 hours. The formulation of the liquid medium was the same as in Example 1.

[0067] After the Penicillium heterophylla CGMCC3.5724 and ATCC52210 were activated on the slant, they were inoculated in the liquid medium and cultured at 30° C. for 72 hours. The formulation of the liquid medium was the same as that in Example 1.

[0068] Viable bacteria count, t...

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PUM

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Abstract

The invention discloses a preparation method of a compound microbial flocculant. The compound microbial flocculant comprises the following materials: Bacillus mucilaginosus, Bacillus subtilis, Saccharomyces cerevisiae and Penicillium diversum, wherein in the compound microbial flocculant, the total bacteria count of microbes is more than one billion per gram; Bacillus mucilaginosus accounts for 30-50% of the total bacteria count; Bacillus subtilis accounts for 10-25% of the total bacteria count; Saccharomy cescerevisiae accounts for 20-40% of the total bacteria count; and Penicillium diversum accounts for 10-20% of the total bacteria count. The method comprises the following steps: amplifying culture and solidifying compound microbial flocculant. The method improves the survival rate of the compound microbial flocculant.

Description

technical field [0001] The invention relates to a flocculant, a preparation method and application of the flocculant, in particular to a composite microbial flocculant, and a preparation method and application of the composite microbial flocculant. Background technique [0002] Landfill leachate refers to the moisture contained in the garbage itself in the landfill, the rain and snow water and other moisture entering the landfill, deducting the saturated water holding capacity of the garbage and the covering soil, and passing through the garbage layer and the covering soil. A high-concentration wastewater that is difficult to treat. Its properties mainly depend on various factors such as the composition of waste, landfill time, climate conditions and landfill design. Generally speaking, it has the following characteristics: ①The water quality is complex and the concentration of organic matter is high; ②The water quality is unstable and the range of change is large. ; ③ The ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N1/14C12N11/10C12N11/08C02F3/34C12R1/07C12R1/125C12R1/865C12R1/80
Inventor 李南华赵海泉曾苏胡子全李春燕沈超
Owner 无锡格莱生物技术有限公司
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