Bacillus subtilis and feed additive containing same

A technology of Bacillus subtilis and feed additives, applied in the field of functional microorganism screening, can solve the problems of aquatic animal residues, drug-resistant strains, losses, etc., and achieve the effects of reducing the occurrence of diseases, improving digestibility, and promoting animal growth

Active Publication Date: 2016-03-02
青岛玛斯特生物技术有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the intensification and high-density scale of aquaculture is expanding day by day, the deterioration of the breeding environment and the harm of diseases have brought huge economic losses to the aquaculture industry.
The traditional method ...

Method used

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  • Bacillus subtilis and feed additive containing same
  • Bacillus subtilis and feed additive containing same

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Isolation, screening and identification of embodiment 1 bacterial strain

[0013] 1. Sample

[0014] The water samples were collected from the culture pond of Penaeus vannamei farm in Jiaozhou, Qingdao.

[0015] 2. Culture medium:

[0016] 1) 2216E seawater medium: 5g protein, 1g yeast extract, 0.01g iron phosphate, 1000ml seawater, pH7.6-7.8, prepare solid medium, add 15-20g agar, and sterilize at 121°C for 20min.

[0017] 2) TSB medium: tryptone soybean broth 30g, sodium chloride 15g, distilled water 1000ml, pH7.4-7.6, prepare solid medium, add 15-20g agar, sterilize at 121°C for 20min.

[0018] 3) MRS medium: peptone 10g, beef extract 10g, glucose 20g, yeast powder 5g, Tween-801ml, potassium dihydrogen phosphate 2g, diammonium citrate 2g, sodium acetate 5g, magnesium sulfate 0.58g, manganese sulfate 0.15g , 1000ml of distilled water, pH6.2-6.4, add 15-20g of agar to prepare a solid medium, and sterilize at 115°C for 20min.

[0019] 4) Starch hydrolysis medium: pe...

Embodiment 2

[0035] Example 2: Effects of Bacillus subtilis SY12 on immunity and growth of grass carp

[0036] Healthy grass carp were selected and randomly divided into two groups—the control group and the experimental group. Each group had three replicates, and each replicate had 30 grass carp (initial body weight 50.86±2.35). The control group was fed with basal feed, and the feed of the experimental group was basal feed with 10 7 CFU / g Bacillus subtilis SY12. The bait was fed daily at 3% of the body weight of the grass carp, and the bait was fed three times at 08:00, 12:00 and 19:00 every day, with a period of 45 days. The experiment was carried out in an indoor circulating water system, during which the water temperature was 23-25°C, pH 7.4-7.8, and dissolved oxygen was not less than 7.5mg / L.

[0037] After the feeding experiment, the feeding was stopped for 24 hours, and 10 fish were randomly selected for each repetition for detection. Blood was collected from the caudal vein, and...

Embodiment 3

[0042] Embodiment 3: The compound additive comprising Bacillus subtilis SY12 affects the growth, immunity and digestion of Penaeus vannamei

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Abstract

The invention relates to the technical field of function microorganism screening and particularly provides novel bacillus subtilis SY12 with the preservation number being CCTCC No: M2015483. The bacillus subtilis SY12 has the advantages that the bacillus subtilis SY12 is capable of effectively inhibiting pathogenic bacteria such as vibrio harveyi and vibrio anguillarum, improving immunity of aquaculture animals and reducing diseases; the bacillus subtilis SY12 with high proteinase-producing and amylase producing capabilities can serve as a feed additive, so that digestive ability of the aquaculture animals is improved remarkably, feed use ratio is increased remarkably, and animal growth is promoted.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a strain of Bacillus subtilis and a feed additive containing the strain. Background technique [0002] With the increase in demand for aquatic products and the international development of aquatic product trade, the aquaculture industry has developed by leaps and bounds in recent years. However, the intensification and high-density scale of aquaculture is expanding day by day, the deterioration of the aquaculture environment and the harm of diseases have brought huge economic losses to the aquaculture industry. The traditional way to prevent and control diseases is mainly to use antibiotics. The use of antibiotics is easy to produce drug-resistant strains, and there are residual problems in aquatic animals. Therefore, it is necessary to find alternatives to antibiotics. [0003] Probiotics can participate in the microbial balance in animals, directly b...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18C12R1/125
Inventor 周怡于朝磊许宏伟苟万里陈琳
Owner 青岛玛斯特生物技术有限公司
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