Bacillus subtilis and application thereof

A technology of bacillus subtilis and its composition, applied in the field of bacillus subtilis and its application, can solve the problems of odor generation, long time for corpse treatment, etc.

Active Publication Date: 2017-05-31
福建洛东生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the use of biodegradation method to deal with animal carcasses, but th

Method used

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  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Organic fertilizer fermentation test

[0032] This experiment has 2 control groups and 1 test group. Each group composted 3 groups of organic fertilizer at a weight ratio of 85% pig manure and 15% straw. Control group 1 did not add any bacteria; control group 2 added 100g of Bacillus subtilis with the preservation number of CCTCCM2016441 to each cubic of compost raw materials (The number of viable bacteria is 1.0×10 10 CFU / g); the test group added 100g of Bacillus subtilis with the preservation number of CCTCC M2016441 (the number of viable cells is 1.0×10 10 CFU / g) and 100g of Bacillus subtilis with the preservation number of CCTCC M2016443 (the number of viable bacteria is 1.0×10 9 CFU / g); the 3 groups of organic fertilizers are mixed and piled up and turned over every other day.

[0033] The fermentation time of control group 1 was 28 days, of which the 3rd to 12th day was the high temperature period, and the temperature during the high temperature period was 58...

Embodiment 2

[0040] Example 2 Strawberry planting test

[0041] Two groups of greenhouse strawberries were selected, each with a strawberry planting area of ​​100 square meters, applied when the strawberries were planted, and harvested 6 months later. The control group was applied with the organic fertilizer fermented by the control group 2 in Example 1, and the test group was applied with the organic fertilizer fermented by the test group in Example 1.

[0042] After 180 days of sowing, the strawberries were harvested. The strawberry yield per mu in the control group was 430 kg, and the yield per mu in the test group was 508 kg. The yield of strawberries in the test group was 18% higher than that of the control group. The test results show that adding Bacillus subtilis with the preservation number of CCTCC M2016443 to the organic fertilizer pile can significantly increase the efficiency of compost and soil fertility.

Embodiment 3

[0043] Example 3 Composition for biological fermentation bed

[0044] 100g Bacillus subtilis with the preservation number of CCTCC M2016441 (the number of viable bacteria is 1.0×10 10 cfu / g), 100g of Bacillus subtilis with the preservation number of CCTCC M2016443 (the number of viable bacteria is 5.0×10 8 cfu / g), mixed uniformly with 800g zeolite powder to obtain 1000g biological fermentation bed composition, which is expanded in the same proportion during production.

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Abstract

The invention relates to bacillus subtilis preserved in a CCTCC with a preservation number of CCTCC M2016443 and application of the bacillus subtilis. The bacillus subtilis has higher enzymatic productivity in a microaerobic environment, and the activity of lipase is higher; the bacillus subtilis realizes the effects of reducing volatilization of ammonia gas and protecting fertilizer efficiency in fermentation of organic fertilizer; in application of deodorization and bacteriostasis of a raising farm, the number of coliform groups can be reduced, harmful microbes in environment are strongly inhibited, ecological balance of environment is adjusted, and the health of a raising environment is benefited; in application of harmless treatment of livestock and poultry corpses, oil can be effectively decomposed and exhaust of stink is reduced; in application of a biological fermentation bed, pig manure can be decomposed more thoroughly, and harmful gases such as diffused ammonia gas is reduced.

Description

Technical field [0001] The invention relates to the field of microbial applications, in particular to a Bacillus subtilis and its application. Background technique [0002] Bacillus subtilis is a kind of Bacillus. A single cell is 0.7~0.8×2~3 microns, and the color is even. There is no capsule, the flagellum is peripheral, and it can move. Gram-positive bacteria, spores 0.6-0.9×1.0-1.5 microns, elliptical to columnar, located in the center of the cell or slightly to the side, the cell does not expand after spore formation. The surface of the colony is rough and opaque, white or slightly yellow, and wrinkles are often formed when growing in liquid medium. Aerobic bacteria can use protein, various sugars and starches to decompose tryptophan to form indole. It is widely used in genetics research, and the synthesis pathway and regulation mechanism of purine nucleotides of this bacteria are relatively clear. Widely distributed in soil and decaying organic matter, it is easy to mu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F15/00C05F11/08C12R1/125
CPCC05F3/00C05F11/08C12N1/205C12R2001/125C05F11/00
Inventor 陈永明李坤卢祯鑫陈丽萍
Owner 福建洛东生物技术有限公司
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