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Bacillus subtilis for degrading aflatoxin B1, and application of bacillus subtilis

A Bacillus subtilis, aflatoxin technology, applied in the application of Bacillus subtilis, the field of Bacillus subtilis, can solve the problem that the degradation effect of high concentration AFB1 needs to be improved, etc.

Active Publication Date: 2017-09-26
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Bacillus subtilis ANSB060 and Bacillus subtilis ANSB324 strains only have a higher degradation rate (more than 80%) to lower concentrations of AFB1 (final concentration 0.5 μg / mL), and the degradation effect on high concentrations of AFB1 needs to be improved

Method used

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  • Bacillus subtilis for degrading aflatoxin B1, and application of bacillus subtilis
  • Bacillus subtilis for degrading aflatoxin B1, and application of bacillus subtilis
  • Bacillus subtilis for degrading aflatoxin B1, and application of bacillus subtilis

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Embodiment 1

[0024] Bacillus subtilis S1 for degrading aflatoxin B1, deposit number: CCTCC NO: M 2016390, deposit date: July 12, 2016, depository unit: China Center for Type Culture Collection (CCTCC), deposit Address: Wuhan, China. Wuhan University (Wuhan University Collection Center, Luojia Mountain, Wuchang, Wuhan City, Hubei Province).

Embodiment 2

[0026] The application of Bacillus subtilis S1 (CCTCC NO: M 2016390) in degrading aflatoxin B1 includes: taking Bacillus subtilis S1 for fermentation and culturing, and the obtained fermentation liquid is used to degrade aflatoxin B1. Alternatively, the fermentation broth is centrifuged (to remove bacteria), and the active protein in the supernatant is separated, and the active protein is used to degrade aflatoxin B1.

Embodiment 3

[0028] The application of Bacillus subtilis S1 (CCTCC NO: M 2016390) in the preparation of biological feed additives for degrading aflatoxin B1 includes: taking Bacillus subtilis S1 for fermentation and culture, centrifuging after cultivation to obtain supernatant; The active protein is separated and mixed with auxiliary materials to obtain a biological feed additive.

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Abstract

The invention discloses bacillus subtilis for degrading aflatoxin B1 and application of the bacillus subtilis, and belongs to the field of biotechnologies. Primary screening is carried out by the aid of courmarin which is used as a unique carbon source and a unique energy source, AFB1 (aflatoxin B1) degradation rates of strains are used as secondary screening indexes, and accordingly the bacillus subtilis S1 (CCTCC NO:M 2016390) capable of efficiently degrading the AFB1 can be obtained by means of screening. The bacillus subtilis and the application have the advantages that the AFB1 degradation rate of strain fermentation broth can reach 61.36%; the degradation rate can reach 80.26% after the bacillus subtilis is cultured according to an inoculation amount of 10%; the degradation rate can reach 85.65% after fermented supernatant is concentrated by 8 times; as shown in research, the aflatoxin B1 can be degraded by extracellular enzymes secreted by the bacillus subtilis S1, and accordingly the strain fermentation broth or active proteins separated from the strain fermentation broth can be used for degrading the aflatoxin B1 or preparing biological additives for degrading the aflatoxin B1.

Description

technical field [0001] The invention relates to a bacillus subtilis for degrading aflatoxin B1, and also relates to the application of the bacillus subtilis, which belongs to the field of biotechnology. Background technique [0002] Aflatoxins (AFT) are mainly highly toxic secondary heterocyclic metabolites produced by various fungi such as Aspergillus flavus and Aspergillus parasiticus. Due to improper storage and transportation, grain, feed and food are prone to mildew and produce aflatoxin. Among the aflatoxins, AFB1 is the most toxic and has strong carcinogenic, teratogenic and immunosuppressive effects on humans and animals. Therefore, solving the problem of aflatoxin pollution in grain and feed is of great significance to improving food safety and ensuring animal health. [0003] At present, the removal methods of aflatoxin in grain and feed mainly include physical, chemical and biological methods. However, the traditional physical and chemical methods have many sho...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L5/20A23K10/18C12R1/125
CPCC12N1/205C12R2001/125
Inventor 王彦彬魏单平闫峰宾蒋瑞瑞李转见田亚东刘小军康相涛
Owner HENAN AGRICULTURAL UNIVERSITY
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