Fish hypoxia-tolerance gene and application thereof

A technology for tolerance and fish, applied in genetic engineering, plant genetic improvement, application, etc., can solve the problems of inability to provide candidate genes and the small number of anti-stress genes, so as to avoid food safety and bioethical concerns, and improve hypoxia Survival rate, the effect of increasing the expression of antioxidant proteins

Active Publication Date: 2016-09-28
CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

[0006] However, most of the exogenous genes reported to be transferred into the fish genome are growth hormone genes and fluorescent protein genes used in ornamental fish. The number of stress-resistant genes is very small, and only one case of hypoxia-resistant transgene has been reported. Transferring the hemoglobin gene VHb of Vibrella hyaline into zebrafish (Guan B et al., MarBiotechnol, 2011, 13:336–344)
The main reason is that we currently know too little about the genes that are significantly associated with hypoxia tolerance traits in fish, and we cannot provide candidate genes for genetic manipulation

Method used

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  • Fish hypoxia-tolerance gene and application thereof
  • Fish hypoxia-tolerance gene and application thereof
  • Fish hypoxia-tolerance gene and application thereof

Examples

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Embodiment 1

[0060] 1. GWAS analysis of carp hypoxia tolerance traits

[0061] Hypoxia tolerance traits vary significantly among different species and geographic groups of common carp. The F10 hybrid descendants of the hypoxia-tolerant variety Mirror carp and the hypoxia-sensitive variety Pouch red carp were selected as the experimental group, and the experimental individuals were reared in the same pond until they were 3 months old, and then moved into an indoor temporary breeding tank for 7 days at 25°C to adapt Environment, select individuals with no trauma, no infection, normal swimming and feeding for hypoxic experiments. Oxygenation with an air pump to control the dissolved oxygen concentration above 6.0mg / L, stop oxygenation after 24 hours of fasting, so that the dissolved oxygen concentration continues to decline naturally, and collect venous blood samples one by one when the individual is hypoxic and comatose. The average body weight of the experimental individuals was 107±20g, a...

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Abstract

The invention discloses a nucleotide sequence with the function of enhancing hypoxia tolerance of fish. The base sequence of the nucleotide sequence is disclosed as (a) or (b) or (c) or (d): (a) base sequence disclosed as SEQ ID NO:1; (b) base sequence disclosed as SEQ ID NO:2; (c) base sequence for coding the protein with the amino acid sequence disclosed as SEQ ID NO:3; or (d) nucleotide sequence for hybridizing with (a) or (b) defined nucleotide sequence under strict hybridization conditions and coding mercapto-specific oxidation-resistant protein. The invention also discloses application of the nucleotide sequence protein, recombinant vector or host cell in enhancing hypoxia tolerance of fish and breeding hypoxia-tolerance transgenic fish varieties. When being integrated into the receptor fish genome as a transplanting gene and effectively expressed, the fish hypoxia-tolerance gene can obviously enhance the hypoxia tolerance of the receptor fish cells and organisms.

Description

technical field [0001] The invention belongs to the technical field of fish genetic engineering, in particular to a fish hypoxia tolerance gene and its application. Background technique [0002] Under ordinary atmospheric pressure, the solubility of oxygen in water is only equivalent to 1 / 30 of the oxygen content in the same volume of air, and the diffusion rate of oxygen in water is much slower than in air. Vegetation or ice cover, temperature gradient, and salinity gradient can all cause local The oxygen content of the water body dropped sharply. Due to the limited oxygen supply in the water body, the low-oxygen tolerance of cultured species has become an important factor affecting the density of aquaculture. For low-oxygen-tolerant fish such as snakehead, mirror carp, etc., through reasonable optimization of the breeding model, "one pond water" can be realized. For high-efficiency and intensive farming of salmon, trout and tongue sole that are not resistant to low oxygen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08C12N15/53C12N15/85C12N5/10A01K67/027
CPCA01K67/0275A01K2207/05A01K2217/05A01K2227/40C12N9/0065C12Y111/01
Inventor 赵紫霞徐鹏张研江炎亮李尚琪孙晓晴
Owner CHINESE ACAD OF FISHERY SCI
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