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Application of mogroside IIE in preparation of antioxidant food, health care products or drugs

A mogroside, oxidized food technology, applied in the functions, applications, food ingredients and other directions of food ingredients, can solve the problem of low utilization rate of mogroside IIE, achieve less toxic side effects, broad market prospects, and scavenge ROS.

Pending Publication Date: 2020-10-02
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the technical problem that the utilization rate of mogroside IIE is low at present, the purpose of the present invention is to provide a new application of mogroside IIE in the preparation of antioxidant food, health products or medicines. After long-term research by the inventors of the present application, it was found that mogroside IIE IIE can scavenge intracellular ROS, resist oxidative stress by regulating PI3K / AKT1-Nrf2 / ARE signaling channel, and enhance the expression of antioxidant proteins, thereby exerting its antioxidant effect

Method used

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  • Application of mogroside IIE in preparation of antioxidant food, health care products or drugs
  • Application of mogroside IIE in preparation of antioxidant food, health care products or drugs
  • Application of mogroside IIE in preparation of antioxidant food, health care products or drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 MTT assay to detect the effect of mogroside IIE on the survival of RAW264.7 cells

[0034] The mouse macrophage RAW264.7 was purchased at the ATCC Model Cell Center in the United States. Cultured in DMEM medium containing 10% fetal bovine serum (FBS) in an incubator at a constant 37°C and filled with a stable 5% CO 2 gas. After cell recovery, cell culture, cell passage and cell counting, cytotoxicity assay was performed using MTT colorimetry to examine the effect of mogroside IIE on the survival of RAW264.7 cells. The specific measurement steps are as follows:

[0035] Prepare a 96-well plate, collect the cells in good condition, and plate the cells to adjust the density to 4×10 4 After confirming that the cells are in good growth state, the drug to be tested can be added with a concentration gradient, set a suitable gradient, set up duplicate wells, and put it into the incubator for 16-48 hours. The old medium in the 96-well plate was removed, replaced wi...

Embodiment 2

[0037] Example 2 ROS activity detection

[0038]When cells are induced with lipopolysaccharide (LPS), the cells will be flooded with ROS, resulting in the accumulation of free radicals in cells or tissues. Excessive oxidation may damage DNA and cause damage to intracellular lipids and proteins, thereby accelerating cell aging. It stimulates the formation of inflammatory mediators and triggers a series of diseases. Therefore, in this example, LPS was used to stimulate cells to construct a cell oxidative damage model, and then the antioxidant effect of mogroside IIE in a mouse macrophage model was detected.

[0039] In this example, the Biyuntian reactive oxygen species assay kit was used to measure ROS, and the kit included DCFH-DA and reactive oxygen species positive control. Cells were treated with LPS and Mogroside IIE and divided into blank group, LPS (40μg / mL) group, LPS+Mogroside IIE (20μM) group, LPS+Mogroside IIE (40μM) group, LPS+Mogroside IIE (80μM) group, LPS+ Mogr...

Embodiment 3

[0043] Example 3 The effect of mogroside IIE on Nrf2 / ARE signaling pathway

[0044] As an important nuclear transcription factor, Nrf2 regulates the expression of antioxidant proteins, thereby preventing oxidative responses triggered by inflammation. When Nrf2 is activated, it will enter the nucleus to combine with ARE, and activate a series of downstream phase II detoxification enzymes and antioxidant genes to remove excess reactive oxygen species in cells. HO-1 is a protective enzyme and rate-limiting enzyme, and its expression is not obvious under normal conditions. When oxidative stress activates the Nrf2 pathway in the organism, the expression of HO-1 increases instantaneously in order to resist external stimuli, so it can be measured by measuring HO-1. HO-1 to determine whether to activate the Nrf2 / ARE signaling pathway. NQO1 is an intracellular enzyme that catalyzes the reduction of various compounds. Its main physiological role is to reduce the detoxification of free...

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Abstract

The invention belongs to the technical field of medicinal natural products, and particularly relates to application of mogroside IIE in preparation of antioxidant food, health care products or drugs.The invention provides the new application of the mogroside IIE and provides a new direction for development and utilization of corsvenor momordica fruits, meanwhile, as a food functional component, the mogroside IIE has smaller toxic and side effects, can be used for preparing antioxidant food, health care products and drugs, and has broad market prospects. The research of the invention finds that the mogroside IIE can clearly remove ROS in cell tissue and shows the antioxidant capacity of the mogroside IIE. The mogroside IIE can activate a Nrf2 / ARE signal path, activate downstream antioxidant proteins and the expression of phase II detoxification enzymes: Nrf2, HO-1 and NQO1. The mogroside IIE can also inhibit the activation of a PI3K / AKT1 signal path, thereby exerting the antioxidant effect of the mogroside IIE.

Description

technical field [0001] The invention belongs to the technical field of medicinal natural products, in particular to the application of mogroside IIE in the preparation of antioxidant food, health care product or medicine. Background technique [0002] Corsvenor Momordica Fruit is a perennial herb of the Cucurbitaceae family, mainly distributed in parts of southern my country. In China, the plant is prized as a sweet fruit and is widely used in medicinal and food sweeteners. [0003] Mogroside IIE (Mogroside IIE) is a typical triterpenoid saponin with a bitter taste. It is the most important component in the fruit less than 50 days old. This bitter compound slowly disappears when the fruit matures to 70 days. It is also the precursor and metabolite of the commercially available sweetener Mogroside V. As a low-calorie food additive, mogroside V has become one of the most potential chemopreventive substances due to its antioxidant, anti-carcinogen and anti-inflammatory proper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P39/06A23L33/105
CPCA61K31/704A61P39/06A23L33/105A23V2002/00A23V2200/30A23V2250/21
Inventor 覃思刘嘉祺邹陆妍
Owner HUNAN AGRICULTURAL UNIV
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