Tegillarca granosa hemoglobin alpha helix antibacterial peptide and application thereof

A hemoglobin, alpha helix technology, applied in the directions of hemoglobin/myoglobin, application, protein-containing food ingredients, etc., can solve the problems of unstable alpha helix structure, loss of bacteriostatic effect, and easy damage.

Active Publication Date: 2021-06-25
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different from traditional antibiotics, most antimicrobial peptides play a role by destroying bacterial cell membranes, but their α-helical structure is unstable under medium and high-intensity ultrasonic conditions, and it is easily destroyed and loses its antibacterial effect

Method used

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  • Tegillarca granosa hemoglobin alpha helix antibacterial peptide and application thereof
  • Tegillarca granosa hemoglobin alpha helix antibacterial peptide and application thereof
  • Tegillarca granosa hemoglobin alpha helix antibacterial peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Optimization of ultrasonic conditions

[0026] The diluted bacterial solution was mixed with the peptide and placed in an ultrasonic water bath at a temperature of 37°C. Using ultrasonic frequency, ultrasonic power and ultrasonic time as parameters, the antibacterial activity and other antibacterial parameters of the antimicrobial peptide TGH2 against Escherichia coli were determined under different conditions. According to the needs of ultrasonic sound perforation mechanism, the ultrasonic power should be controlled at 5.0W / cm as much as possible 2 In the following, the α-helical structure of the antimicrobial peptide TGH2 may remain stable.

Embodiment 2

[0027] Embodiment 2: Screening of antimicrobial peptides

[0028] First log on to the NCBI (National Center for Biotechnology Information) website to find the cockles hemoglobin sequence, and then use the Antibacterial Peptide Prediction Online Server AntiBP to predict the possible antibacterial regions of the hemoglobin sequence, and conduct an investigation on the charge, hydrophobicity and reliability of the antibacterial sequence. analyze. Finally, the amino acid sequence AEFLREKLGDKCTDR was selected and named as TGH2. The hemoglobin sequence of cockles consists of 152 amino acids, and the TGH2 sequence is located at sequence number 119-136. Using the online server APD3 to calculate the hydrophobicity of the TGH2 sequence, the total hydrophobicity ratio is 33%. Finally, the antibacterial activity of TGH2 was verified.

Embodiment 3

[0029] Embodiment 3: Escherichia coli growth curve

[0030] Inoculate 20uL of the activated strain into 20mL nutrient broth liquid medium to reach 10 2-4 CFU / mL concentration, then at 0.5W / cm 2 After ultrasonic treatment for 1 hour, take 20ul (0, 1, 2, 3, 4, 5, 6, 7, 8 hours) samples for plate counting and observe the growth curve. like figure 1 As shown, there was no obvious damage to Escherichia coli after low-intensity ultrasonic treatment, and the growth trend was basically consistent with that under the culture conditions.

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Abstract

The invention relates to an antibacterial peptide, in particular to a tegillarca granosa hemoglobin alpha-helix antibacterial peptide and application thereof. The antibacterial peptide comprises an amino acid sequence selected from SEQ ID NO: 1 or consists of the amino acid sequence, has an effective antibacterial effect, and enhances the antibacterial activity of the antibacterial peptide on bacteria through low-intensity ultrasonic processing. The TGH2 subjected to low-intensity ultrasonic-assisted treatment has a strong inhibition effect on bacteria, and meanwhile, the alpha helical structure of the antibacterial peptide TGH2 is kept stable. The invention provides an experimental basis for a low-intensity ultrasonic sterilization method and TGH2 as a food preservative.

Description

technical field [0001] The invention relates to an antibacterial peptide, in particular to a cockle hemoglobin alpha helical antibacterial peptide and application thereof. Background technique [0002] Foodborne illness caused by pathogenic microorganisms is a major concern for consumers, industry and regulators. Foodborne pathogenic bacteria pose a threat to human health in the process of food raw materials, processing and storage. [0003] Ultrasound is a sound wave with a frequency exceeding 20kHz. As a form of vibration energy with good directionality, penetration and reflectivity, it has become a cutting-edge antibacterial technology in the food industry. It is generally believed that the antibacterial mechanism of ultrasound can be divided into three mechanisms: acoustic cavitation, sonoporation and sonochemistry, and the acoustic cavitation and sonochemical mechanisms can only be realized under medium and high-intensity ultrasound. It has been reported that medium a...

Claims

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Application Information

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IPC IPC(8): C07K14/805C12N15/12A23L3/30A23L3/3526
CPCC07K14/805A23L3/3526A23L3/30A23V2002/00A23V2250/55A23V2200/10A23V2300/48
Inventor 杨燊袁紫金戴静怡石林凡郑明静翁武银
Owner JIMEI UNIV
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