A kind of mud cockles hemoglobin alpha helix antimicrobial peptide and its application

A technology of hemoglobin and antimicrobial peptides, applied in hemoglobin/myoglobin, application, protein-containing food ingredients, etc., can solve problems such as loss of antibacterial effect, easy to be destroyed, unstable α helix structure, etc.

Active Publication Date: 2022-08-05
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different from traditional antibiotics, most antimicrobial peptides play a role by destroying bacterial cell membranes, but their α-helical structure is unstable under medium and high-intensity ultrasonic conditions, and it is easily destroyed and loses its antibacterial effect

Method used

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  • A kind of mud cockles hemoglobin alpha helix antimicrobial peptide and its application
  • A kind of mud cockles hemoglobin alpha helix antimicrobial peptide and its application
  • A kind of mud cockles hemoglobin alpha helix antimicrobial peptide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Optimization of ultrasonic conditions

[0026] The diluted bacterial solution and peptide were mixed and placed in an ultrasonic water bath at a temperature of 37°C. Using ultrasonic frequency, ultrasonic power and ultrasonic time as parameters, the antibacterial activity of antimicrobial peptide TGH2 against Escherichia coli and other antibacterial parameters were determined under different conditions. According to the needs of the ultrasonic sonoporation mechanism, the ultrasonic power should be controlled at 5.0W / cm as far as possible. 2 In the following, the α-helical structure of the antimicrobial peptide TGH2 may remain stable.

Embodiment 2

[0027] Example 2: Screening of antimicrobial peptides

[0028] First, log on to the website of NCBI (National Center for Biotechnology Information) to find the hemoglobin sequence of the slug, and then use the antibacterial peptide prediction online server AntiBP to predict the possible antibacterial regions of the hemoglobin sequence, and conduct the charge, hydrophobicity and reliability of the antibacterial sequence. analyze. The amino acid sequence AEFLREKLGDKCTDR was finally screened and named as TGH2. The hemoglobin sequence of the slug is composed of 152 amino acids, and the TGH2 sequence is located in the sequence number 119-136. Using the online server APD3 to calculate the hydrophobicity of the TGH2 sequence, the total hydrophobicity ratio was 33%. Finally, the antibacterial activity of TGH2 was verified.

Embodiment 3

[0029] Example 3: E. coli growth curve

[0030] Inoculate 20uL of the activated strain into 20mL of nutrient broth liquid medium to 10 2-4 CFU / mL concentration, then at 0.5W / cm 2 Under ultrasonic treatment for 1h, take 20ul (0, 1, 2, 3, 4, 5, 6, 7, 8 hours) samples for plate counting and observe the growth curve. like figure 1 As shown, the E. coli treated with low-intensity sonication did not have obvious damage, and the growth trend was basically the same as that under the culture conditions.

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Abstract

The present invention relates to an antibacterial peptide, in particular to an antibacterial peptide of hemoglobin hemoglobin alpha helix and its application. The antibacterial peptide comprises or consists of an amino acid sequence selected from SEQ ID NO: 1, has an effective bacteriostatic effect, and enhances its bacteriostatic activity on bacteria through low-intensity ultrasonic processing. Low-intensity ultrasonic-assisted treatment of TGH2 has a strong inhibitory effect on bacteria, while the α-helix structure of antimicrobial peptide TGH2 remains stable. The invention provides an experimental basis for the sterilization method of low-intensity ultrasound and the use of TGH2 as a food preservative.

Description

technical field [0001] The present invention relates to an antibacterial peptide, in particular to an antibacterial peptide of hemoglobin hemoglobin alpha helix and its application. Background technique [0002] Foodborne illnesses caused by pathogenic microorganisms are a major concern for consumers, industry and regulators. Foodborne pathogens pose a threat to human health during food raw materials, processing and storage. [0003] Ultrasound is a sound wave with a frequency of over 20kHz. As a form of vibrational energy with good directionality, penetration and reflectivity, it has become a cutting-edge antibacterial technology in the food industry. It is generally believed that the mechanism of ultrasonic bacteriostasis is divided into three mechanisms: acoustic cavitation, acoustic perforation and sonochemistry. Medium and high-intensity ultrasound has been reported to inhibit E. coli O157:H7 and Listeria monocytogenes in almond milk and prolong shelf life under refri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/805C12N15/12A23L3/30A23L3/3526
CPCC07K14/805A23L3/3526A23L3/30A23V2002/00A23V2250/55A23V2200/10A23V2300/48
Inventor 杨燊袁紫金戴静怡石林凡郑明静翁武银
Owner JIMEI UNIV
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