Anti-prostate-cancer tegillarca granosa protein polypeptide as well as preparation method and application thereof

An anti-prostate cancer, protein polypeptide technology, applied in the field of anti-prostate cancer cockle protein polypeptide, can solve the problem of high-activity anti-prostate cancer polypeptides that have not been reported, and achieve strong anti-prostate cancer cell proliferation inhibition and strong anti-prostate cancer activity , easy to digest and absorb

Inactive Publication Date: 2015-04-22
ZHEJIANG OCEAN UNIV
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Problems solved by technology

[0003] At present, the applicant has found that the research on the preparation of anti-prostate cancer polypeptides using cockles as raw materials by enzymatic hydrolysis technology is in a blank stage, and the preparation of high-activity anti-prostate cancer polypeptides and their applications using enzymatic hydrolysis products as materials has never been seen. to report

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  • Anti-prostate-cancer tegillarca granosa protein polypeptide as well as preparation method and application thereof
  • Anti-prostate-cancer tegillarca granosa protein polypeptide as well as preparation method and application thereof
  • Anti-prostate-cancer tegillarca granosa protein polypeptide as well as preparation method and application thereof

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[0026] Examples: (1) Blood cockles were used as raw materials, cockle meat tissue was minced, and isopropanol was added at a solid-to-liquid ratio of 1 g: 4 mL. After degreasing at 35 °C for 48 h, centrifuged at 6 000 r / min for 15 min, take the precipitate and dry it to get the defatted cockle meat;

[0027] (2) Add phosphate buffer (0.02 mol / L, pH 7.0) to the defatted cockle meat at a solid-to-liquid ratio of 1 g: 20 mL, and add neutral protease (enzyme activity ≥ 1.5 AU / g), enzymatic hydrolysis at 60°C for 6 h;

[0028] (3) Heat the enzymatic solution to 95°C for 10 min, centrifuge at 12 000 r / min for 10 min, remove the precipitate, and take the supernatant; the supernatant is subjected to ultrafiltration and chromatography in turn to obtain the anti-prostate cancer polypeptide , using amino acid sequence analysis and mass spectrometry to determine its structure, the specific process is:

[0029] ①Ultrafiltration: The supernatant was subjected to ultrafiltration treatment...

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Abstract

The invention discloses an anti-prostate-cancer tegillarca granosa protein polypeptide as well as a preparation method and application thereof. The amino acid sequence of the anti-prostate-cancer tegillarca granosa protein polypeptide is Pro-Gln-Trp-Pro-Pro (PEWPP), the ESI / MS (electron spray ionization / mass spectrometry) detection shows that the molecular weight of the anti-prostate cancer tegillarca granosa protein polypeptide is 624.65Da. The preparation method of the anti-prostate-cancer tegillarca granosa protein polypeptide comprises the steps of: mincing tissues, hydrolyzing with an enzyme, ultra-filtrating and chromatographing. The prepared high-activity anti-prostate-cancer tegillarca granosa protein polypeptide with the amino acid sequence of Pro-Gln-Trp-Pro-Pro (PEWPP) has a significant effect of inhibiting the proliferation of human prostate cancer cells PC-3 and can be used as an anti-prostate cancer medicine or health food.

Description

technical field [0001] The invention relates to an anti-prostate cancer cockle protein polypeptide, a preparation method of the anti-prostate cancer cockle protein polypeptide, and an anti-tumor application of the anti-prostate cancer cockle protein polypeptide. Background technique [0002] blood cockles ( Tegillarca granosa ) belongs to the mollusk "valvolabranches", which are distributed in all parts of the north and south coasts of my country. The blood cockles are purple-red in color and extremely delicious, and are very popular among consumers in Jiangsu, Zhejiang, Fujian and other places. In addition, traditional herbal works record that cockle meat has the effects of nourishing qi and blood, invigorating the spleen and stomach, dispelling stagnation and eliminating phlegm, and is often used for diseases such as lumps in the abdomen, old phlegm accumulation and so on. [0003] At present, the applicant has found that the research on the preparation of anti-prosta...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/22C07K1/18C07K1/16A61K38/08A61P35/00A23L1/305A23L33/18
Inventor 王斌潘欣迟长凤胡发远陈荫
Owner ZHEJIANG OCEAN UNIV
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