Fingerprint identification method for tegillarca granosa in different sea area
A mud cockles and fingerprint technology is applied in the field of fingerprint identification of mud cockles in different sea areas, which can solve the problems of inconvenient identification of cultured species, difficulty in distinguishing Fenghua mud cockles, and high price, and achieve the effect of easy identification and breeding.
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[0027] A fingerprint identification method for cockles in different sea areas is to randomly get cockles in each sea area respectively, and then process the cockles in each sea area in the following steps:
[0028] a. Tissue selection: Randomly select 10 cockles from Yueqing, Zhejiang, divide and weigh the digestive gland tissue of each cockle to obtain 1g tissue;
[0029] B, extract enzyme liquid: put the digestive gland tissue of each cockle into glass homogenizer respectively, then add 0.05% mercaptoethanol aqueous solution 3ml, make homogenate under ice bath condition; Put homogenate into temperature In a refrigerated centrifuge at 4°C, centrifuge at 12,000rpm for 30min, extract the supernatant after centrifugation, and the supernatant is the enzyme solution;
[0030] c. Electrophoresis: Divide the enzyme solution into two parts, add an appropriate amount of bromophenol blue indicator to each part of the enzyme solution, and then use polyacrylamide gel electrophoresis with...
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