Fingerprint identification method for tegillarca granosa in different sea area

A mud cockles and fingerprint technology is applied in the field of fingerprint identification of mud cockles in different sea areas, which can solve the problems of inconvenient identification of cultured species, difficulty in distinguishing Fenghua mud cockles, and high price, and achieve the effect of easy identification and breeding.

Inactive Publication Date: 2008-05-07
NINGBO UNIV
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AI Technical Summary

Problems solved by technology

Mud cockles are one of the leading economically cultured shellfish in the aquaculture industry. They are delicious and have high nutritional value, and are deeply loved by people. The blood color of the cockles is bright red and lighter, while the blood color of the mud cockles in Rongcheng is red, while the blood color of the mud cockles in Guangxi Beihai, Guangdong Yangjiang and Zhanjiang is dark red and darker; and the taste of the mud cockles in Yueqing is more delicious, and the price is correspondingly higher
[0003] Mud cockles are dioecious, sexually mature at 2 years old, and the ratio of shell length to shell height of mature cockles is similar to that of Shantou cockles, Zhanjiang cockles, Korean cockles and Rongcheng cockles, and the number of longitudinal ribs on the shell surface There is no significant difference between them. There is no significant difference in shell length, shell height and number of longitudinal ribs between Fenghua cockles, Yueqing cockles and Fuding cockles. It is difficult to distinguish Shantou cockles, Zhanjiang cockles, Korean cockles and Rongcheng cockles, and it is also difficult to distinguish Fenghua cockles, Yueqing cockles and Fuding cockles
In addition, when the fertilized eggs develop into spat, the spat of mud cockles in various places are basically similar, and it is difficult to distinguish them morphologically, which brings inconvenience to the identification of cultured species of mud cockles

Method used

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  • Fingerprint identification method for tegillarca granosa in different sea area
  • Fingerprint identification method for tegillarca granosa in different sea area

Examples

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Embodiment 1

[0027] A fingerprint identification method for cockles in different sea areas is to randomly get cockles in each sea area respectively, and then process the cockles in each sea area in the following steps:

[0028] a. Tissue selection: Randomly select 10 cockles from Yueqing, Zhejiang, divide and weigh the digestive gland tissue of each cockle to obtain 1g tissue;

[0029] B, extract enzyme liquid: put the digestive gland tissue of each cockle into glass homogenizer respectively, then add 0.05% mercaptoethanol aqueous solution 3ml, make homogenate under ice bath condition; Put homogenate into temperature In a refrigerated centrifuge at 4°C, centrifuge at 12,000rpm for 30min, extract the supernatant after centrifugation, and the supernatant is the enzyme solution;

[0030] c. Electrophoresis: Divide the enzyme solution into two parts, add an appropriate amount of bromophenol blue indicator to each part of the enzyme solution, and then use polyacrylamide gel electrophoresis with...

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Abstract

The invention discloses a fingerprint identification method for tegillarca granosa in different sea areas. The fingerprints are identified through the special albumen of the digestive gland tissue of the tegillarca granosa and the special DNA sequences of the tegillarca granosa, because of the special albumen in the digestive gland tissue of the tegillarca granosa in different sea areas, the enzyme bands of the SOD enzyme and the MDH enzyme are all different, in addition, the special DNA sequences of the tegillarca granosa in different sea areas all have specificity, therefore the places of production of the tegillarca granosa can be identified according to the special albumen of the digestive gland tissue of the tegillarca granosa and the special DNA sequences of the tegillarca granosa, which facilitates to identify and culture tegillarca granosa with delicious flavor and high price.

Description

technical field [0001] The invention relates to identification technology within species, in particular to a fingerprint identification method for cockles in different sea areas. Background technique [0002] Mud cockles (Tegillarca granosa Linnaeus), commonly known as blood cockles, Ning cockles, flower cockles, and grain cockles, belong to the mollusca (Mollusca), lamellibranchia (Lamellibranchia), taxodonta (Taxodonta), cockles (Arcidae) animals, mainly Distributed in countries such as China, South Korea, Thailand, the Philippines, and Malaysia in the Western Pacific, Indian Ocean, and Atlantic Ocean. In my country, it is distributed along the south coast of Shandong Peninsula. Mud cockles are one of the leading economically cultured shellfish in the aquaculture industry. They are delicious and have high nutritional value, and are deeply loved by people. The blood color of the cockles is bright red and light, while the blood color of the mud cockles in Rongcheng is red,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25G01N27/447G01N33/68C12Q1/68
Inventor 李太武苏秀榕
Owner NINGBO UNIV
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