Compound organic fertilizer as well as preparation method and application thereof

Abstract

The invention provides a compound organic fertilizer as well as a preparation method and application thereof, and the compound organic fertilizer is prepared from the following raw materials in parts by weight: 40 to 50 parts of basic fertilizer, 5 to 10 parts of alkaloid compound preparation, 1 to 3 parts of dendrobium nobile stem extract, 2 to 4 parts of carnosic acid nutrient solution, 5 to 8 parts of compound fermentation liquor and 0.8 to 1.2 parts of antibacterial peptide, the alkaloid compound preparation is prepared from nicotine, caffeine, theobromine, sophocarpidine and colchicine according to the mass ratio of 1: (1 to 2): (0.8 to 1.2): (0.5 to 0.8): (1.2 to 1.5); the compound fermentation liquor is prepared from earthworm fermentation liquor and chitin fermentation liquor in a mass ratio of 1: (3-5). According to the compound organic fertilizer disclosed by the invention, the alkaloid compound preparation, the dendrobium stem extract, the carnosic acid nutrient solution, the compound fermentation liquor and the antibacterial peptide are mixed with the basic fertilizer, so that the compound organic fertilizer has a prevention and treatment effect on phomopsis areca leaf spot and banana wilt, the yield increasing effect of bananas is remarkable, and the compound organic fertilizer has relatively good popularization and application values.

Description

Technical Field

[0001] The invention relates to the field of organic fertilizers, and in particular to a composite organic fertilizer and a preparation method and application thereof. Background Art

[0002] my country is a large agricultural country. In agriculture, pests and diseases are mainly controlled by mixing chemical preparations and toxic pesticides and applying them to the soil or crops. Long-term use of large amounts of pesticides and fertilizers has caused pests to develop drug resistance, soil compaction, soil and crop pollution, and a decline in the quality of agricultural products. Pesticide residues exceed the standard, seriously affecting the competitiveness of my country's agricultural products in the international market. Therefore, it is a development trend to develop a safe, effective and environmentally friendly organic fertilizer.

[0003] Among them, betel nut and banana are important economic crops. In recent years, the Phomopsis leaf spot disease of betel nut has seriously affected the betel nut industry. Phomopsis can infect betel nut seedlings and mature trees, and both old and young leaves can be damaged. In severe cases, it causes seedling death and leaf blight. In addition, banana wilt is an important disease in banana production, which can cause serious reduction in banana production or even total crop failure. It is very difficult to prevent and control, and is known as banana cancer. There is currently no cure.

[0004] Dendrobium contains a variety of chemical components, among which the main active ingredients include dendrobium polysaccharides and alkaloids, which are used in the treatment of immune-related diseases. However, it has not been reported that the alkaloids in Dendrobium have a preventive and therapeutic effect on areca nut Phomopsis leaf spot disease and banana wilt disease.

[0005] Carnosic acid is a diterpene phenol compound found in rosemary, sage and other Lamiaceae plants. It has antioxidant, anti-inflammatory, anti-tumor, antibacterial and cardiovascular protection effects. It is safe and low-toxic to humans and animals. It is often used in food preservation, cosmetics and health products. There are no reports on the preventive and therapeutic effects of carnosic acid on areca nut Phomopsis leaf spot disease and banana wilt disease. Summary of the invention

[0006] In view of this, the present invention provides a composite organic fertilizer and a preparation method and application thereof.

[0007] The technical solution of the present invention is achieved in this way:

[0008] A composite organic fertilizer comprises the following raw materials in parts by weight: 40-50 parts of basic fertilizer, 5-10 parts of alkaloid compound preparation, 1-3 parts of Dendrobium nobile stem extract, 2-4 parts of carnosic acid nutrient solution, 5-8 parts of composite fermentation liquid and 0.8-1.2 parts of antimicrobial peptide;

[0009] The alkaloid compound preparation is nicotine, caffeine, theobromine, matrine and colchicine in a mass ratio of 1:1-2:0.8-1.2:0.5-0.8:1.2-1.5;

[0010] The composite fermentation liquid is earthworm fermentation liquid and chitin fermentation liquid in a mass ratio of 1:3-5.

[0011] Furthermore, the preparation method of the carnosic acid nutrient solution includes: adding carnosic acid to vegetable oil and mixing evenly to obtain an oil phase, adding amino acids to water and mixing evenly to obtain an aqueous phase, mixing the oil phase and the aqueous phase, adding polysorbate, and high-speed shearing to obtain a carnosic acid nutrient solution.

[0012] Furthermore, the solid-liquid ratio of carnosic acid to vegetable oil is 1:5-7 g / mL; the solid-liquid ratio of amino acids to water is 1:8-10 g / mL; the volume ratio of the oil phase to the water phase is 1:2-4; the mass ratio of the polysorbate to the oil phase is 0.1-0.3:1; and the high-speed shearing is performed at 2000-3000 rpm for 5-10 min.

[0013] Furthermore, the Dendrobium nobile stem extract contains 1.04-2.12 mg / g of alkaloids.

[0014] Furthermore, the antimicrobial peptides are antimicrobial peptides separated from the fermentation broth of Brevibacillus laterosporus and antimicrobial peptides separated from the fermentation broth of Trichoderma harzianum in a mass ratio of 1:1-1.5.

[0015] Furthermore, the earthworm fermentation liquid is obtained by mixing and stirring chopped hungry earthworms and compound enzymes; the mass ratio of the hungry earthworms to the compound enzymes is 100:0.1-0.5; the compound enzymes are papain and lipase in a mass ratio of 1:1-3; the mixing and stirring is performed at 100-200 rpm and 40-60° C. for 20-30 hours;

[0016] The chitosan fermentation liquid is obtained by mixing and fermenting shrimp shells with a composite bacterial liquid; the mass ratio of the shrimp shells to the composite bacterial liquid is 1:5-10; the effective colony count of the composite bacterial liquid is 106-108 CFU / mL; the composite bacterial liquid contains Lactobacillus bulgaricus and Streptococcus thermophilus in a mass ratio of 3-5:1; the fermentation temperature is 40-50°C and the fermentation time is 12-24h.

[0017] A method for preparing a composite organic fertilizer, the specific steps comprising:

[0018] (1) mixing the alkaloid composite preparation and the Dendrobium nobile stem extract uniformly, adding carnosic acid nutrient solution and composite fermentation liquid, and ultrasonically treating to obtain a mixed material;

[0019] (2) The mixed material and the basic fertilizer are mixed, the pH is adjusted to be neutral, anaerobically fermented, dried and granulated to obtain the target compound organic fertilizer.

[0020] Furthermore, in step (1), the ultrasonic treatment is carried out at 20-40 kHz and 30-40° C. for 1-3 h;

[0021] In step (2), the anaerobic fermentation is first performed at 50-60°C for 1-3 days, and then at 35-45°C for 2-4 days.

[0022] The application of the composite organic fertilizer in preventing and controlling plant diseases.

[0023] Furthermore, the plant diseases are Phomopsis areca leaf spot and banana wilt.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] The composite organic fertilizer of the invention is prepared by mixing a basic fertilizer with an alkaloid compound preparation, a stem extract of Dendrobium nobile, a carnosic acid nutrient solution, a composite fermentation liquid and an antimicrobial peptide to achieve synergistic enhancement. Test examples have shown that the composite organic fertilizer has preventive and therapeutic effects on Phomopsis areca leaf spot disease and banana wilt disease, and has a significant effect on increasing banana yield, and has good promotion and application value. DETAILED DESCRIPTION

[0026] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0027] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0028] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0029] The Dendrobium nobile stem extract of the present invention was purchased from Gansu Yishengxiang Biotechnology Co., Ltd.

[0030] The basic fertilizer of the invention is a fully decomposed fermentation semi-finished product obtained by composting and fermenting straw, humus and feces in a mass ratio of 1:0.2:2 for 20-30 days.

[0031] The vegetable oil of the present invention is any one of olive oil, palm oil, corn oil or sunflower oil.

[0032] The amino acid of the present invention is glutamic acid.

[0033] The nicotine, caffeine, theobromine, matrine and colchicine of the invention are obtained by fermenting agricultural wastes of Solanaceae crops, Theaceae crops, Leguminosae crops and Liliaceae crops.

[0034] The Brevibacillus laterosporus of the present invention was purchased from Noan Gene Technology (Wuhan) Co., Ltd.

[0035] The Trichoderma harzianum of the present invention was purchased from Weifang Ruichen Biotechnology Co., Ltd.

[0036] The Lactobacillus bulgaricus of the present invention was purchased from Xi'an Rongzhen Biotechnology Co., Ltd.

[0037] The thermophilic streptococcus of the present invention was purchased from Xi'an Meihe Biotechnology Co., Ltd.

[0038] Example 1

[0039] A composite organic fertilizer, comprising the following raw materials in parts by weight: 45 parts of basic fertilizer, 8 parts of alkaloid compound preparation (nicotine, caffeine, theobromine, matrine and colchicine in a mass ratio of 1:1.5:1:0.7:1.3), 2 parts of Dendrobium nobile stem extract, 3 parts of carnosic acid nutrient solution, 7 parts of composite fermentation liquid (earthworm fermentation liquid and chitin fermentation liquid in a mass ratio of 1:4), and 1 part of antimicrobial peptide;

[0040] The antimicrobial peptides are antimicrobial peptides isolated from the fermentation broth of Brevibacillus laterosporus and antimicrobial peptides isolated from the fermentation broth of Trichoderma harzianum in a mass ratio of 1:1.3.

[0041] The preparation method of carnosic acid nutrient solution is as follows: carnosic acid is added to vegetable oil at a solid-liquid ratio of 1:6 g / mL and mixed evenly to obtain an oil phase; amino acids are added to water at a solid-liquid ratio of 1:9 g / mL and mixed evenly to obtain an aqueous phase; the oil phase and the aqueous phase are mixed at a volume ratio of 1:3, polysorbate is added (the mass ratio of polysorbate to oil phase is 0.2:1), and sheared at 2500 rpm for 8 minutes to obtain a carnosic acid nutrient solution.

[0042] Preparation method of earthworm fermentation liquid: mix chopped hungry earthworms and complex enzyme (papain and lipase in a mass ratio of 1:2) in a mass ratio of 100:0.3, and stir at 150 rpm and 50°C for 25 hours.

[0043] Preparation method of chitosan fermentation liquid: crush shrimp shells, mix shrimp shells with composite bacterial liquid (effective colony count is 10 7 CFU / mL, Lactobacillus bulgaricus and Streptococcus thermophilus in a mass ratio of 4:1 were mixed and fermented at 45°C for 18h.

[0044] The preparation method of the above-mentioned composite organic fertilizer comprises the following specific steps:

[0045] (1) mixing the alkaloid compound preparation and the stem extract of Dendrobium nobile uniformly, adding carnosic acid nutrient solution and composite fermentation liquid, and ultrasonically treating at 30 kHz and 35° C. for 2 h to obtain a mixed material;

[0046] (2) The mixed material and the basic fertilizer are mixed, the pH is adjusted to be neutral, anaerobically fermented (first at 55° C. for 2 days, then at 40° C. for 3 days), dried and granulated to obtain the target compound organic fertilizer.

[0047] Example 2

[0048] A composite organic fertilizer comprises the following raw materials in parts by weight: 40 parts of basic fertilizer, 5 parts of alkaloid compound preparation (nicotine, caffeine, theobromine, matrine and colchicine in a mass ratio of 1:1:0.8:0.5:1.2), 1 part of Dendrobium nobile stem extract, 2 parts of carnosic acid nutrient solution, 5 parts of composite fermentation liquid (earthworm fermentation liquid and chitin fermentation liquid in a mass ratio of 1:3), and 0.8 parts of antimicrobial peptide;

[0049] The antimicrobial peptides are antimicrobial peptides isolated from the fermentation broth of Brevibacillus laterosporus and antimicrobial peptides isolated from the fermentation broth of Trichoderma harzianum in a mass ratio of 1:1.

[0050] The preparation method of carnosic acid nutrient solution is as follows: carnosic acid is added to vegetable oil at a solid-liquid ratio of 1:5 g / mL and mixed evenly to obtain an oil phase; amino acids are added to water at a solid-liquid ratio of 1:8 g / mL and mixed evenly to obtain an aqueous phase; the oil phase and the aqueous phase are mixed at a volume ratio of 1:2, polysorbate is added (the mass ratio of polysorbate to oil phase is 0.1:1), and sheared at 2000 rpm for 5 minutes to obtain a carnosic acid nutrient solution.

[0051] Preparation method of earthworm fermentation liquid: mix chopped hungry earthworms and complex enzyme (papain and lipase in a mass ratio of 1:1) in a mass ratio of 100:0.1, and stir at 100 rpm and 40°C for 20 hours.

[0052] Preparation method of chitosan fermentation liquid: crush shrimp shells, mix shrimp shells with composite bacterial liquid (effective colony count is 10 6 CFU / mL, Lactobacillus bulgaricus and Streptococcus thermophilus in a mass ratio of 3:1 were mixed and fermented at 40°C for 12h.

[0053] The preparation method of the above-mentioned composite organic fertilizer comprises the following specific steps:

[0054] (1) mixing the alkaloid compound preparation and the stem extract of Dendrobium nobile uniformly, adding carnosic acid nutrient solution and composite fermentation liquid, and ultrasonically treating at 20 kHz and 30° C. for 1 h to obtain a mixed material;

[0055] (2) The mixed material and the basic fertilizer are mixed, the pH is adjusted to be neutral, anaerobically fermented (first fermented at 50° C. for 1 day, then fermented at 35° C. for 2 days), dried and granulated to obtain the target compound organic fertilizer.

[0056] Example 3

[0057] A composite organic fertilizer comprises the following raw materials in parts by weight: 50 parts of basic fertilizer, 10 parts of alkaloid compound preparation (nicotine, caffeine, theobromine, matrine and colchicine in a mass ratio of 1:2:1.2:0.8:1.5), 3 parts of Dendrobium nobile stem extract, 4 parts of carnosic acid nutrient solution, 8 parts of composite fermentation liquid (earthworm fermentation liquid and chitin fermentation liquid in a mass ratio of 1:5), and 1.2 parts of antimicrobial peptide;

[0058] The antimicrobial peptides are antimicrobial peptides isolated from the fermentation broth of Brevibacillus laterosporus and antimicrobial peptides isolated from the fermentation broth of Trichoderma harzianum in a mass ratio of 1:1.5.

[0059] The preparation method of carnosic acid nutrient solution is as follows: carnosic acid is added to vegetable oil at a solid-liquid ratio of 1:7 g / mL and mixed evenly to obtain an oil phase; amino acids are added to water at a solid-liquid ratio of 1:10 g / mL and mixed evenly to obtain an aqueous phase; the oil phase and the aqueous phase are mixed at a volume ratio of 1:4, polysorbate is added (the mass ratio of polysorbate to oil phase is 0.3:1), and sheared at 3000 rpm for 10 minutes to obtain a carnosic acid nutrient solution.

[0060] Preparation method of earthworm fermentation liquid: mix chopped hungry earthworms and complex enzyme (papain and lipase in a mass ratio of 1:3) in a mass ratio of 100:0.5, and stir at 200 rpm and 60°C for 30 hours.

[0061] Preparation method of chitosan fermentation liquid: crush shrimp shells, mix shrimp shells with composite bacterial liquid (effective colony count is 10) at a mass ratio of 1:10 8 CFU / mL, Lactobacillus bulgaricus and Streptococcus thermophilus in a mass ratio of 5:1 were mixed and fermented at 50°C for 24h.

[0062] The preparation method of the above-mentioned composite organic fertilizer comprises the following specific steps:

[0063] (1) mixing the alkaloid compound preparation and the stem extract of Dendrobium nobile uniformly, adding carnosic acid nutrient solution and composite fermentation liquid, and ultrasonically treating at 40 kHz and 40° C. for 3 h to obtain a mixed material;

[0064] (2) The mixed material and the basic fertilizer are mixed, the pH is adjusted to be neutral, anaerobically fermented (first at 60° C. for 3 days, then at 45° C. for 4 days), dried and granulated to obtain the target compound organic fertilizer.

[0065] Comparative Example 1

[0066] The difference from Example 1 is that the amount of raw materials is different, and the rest is consistent with Example 1.

[0067] That is, the composite organic fertilizer of this comparative example comprises the following raw materials in parts by weight: 38 parts of basic fertilizer, 4 parts of alkaloid compound preparation, 4 parts of Dendrobium nobile stem extract, 5 parts of carnosic acid nutrient solution, 4 parts of composite fermentation liquid, and 1.5 parts of antimicrobial peptide.

[0068] Comparative Example 2

[0069] The difference from Example 1 is that the alkaloid compound preparation is replaced by matrine, and the rest is the same as Example 1.

[0070] That is, the composite organic fertilizer of this comparative example includes the following raw materials in parts by weight: 45 parts of basic fertilizer, 8 parts of matrine, 2 parts of Dendrobium nobile stem extract, 3 parts of carnosic acid nutrient solution, 7 parts of composite fermentation liquid (earthworm fermentation liquid and chitin fermentation liquid in a mass ratio of 1:4), and 1 part of antimicrobial peptide.

[0071] Comparative Example 3

[0072] The difference from Example 1 is that the extract of Dendrobium nobile stem is missing, and the rest is consistent with Example 1.

[0073] That is, the composite organic fertilizer of this comparative example includes the following raw materials in parts by weight: 45 parts of basic fertilizer, 8.9 parts of alkaloid compound preparation (nicotine, caffeine, theobromine, matrine and colchicine in a mass ratio of 1:1.5:1:0.7:1.3), 3.3 parts of carnosic acid nutrient solution, 7.7 parts of composite fermentation liquid (earthworm fermentation liquid and chitin fermentation liquid in a mass ratio of 1:4), and 1.1 parts of antimicrobial peptide;

[0074] Comparative Example 4

[0075] The difference from Example 1 is that the carnosic acid nutrient solution is replaced by carnosic acid, and the rest is the same as Example 1.

[0076] That is, the composite organic fertilizer of this comparative example includes the following raw materials in parts by weight: 45 parts of basic fertilizer, 8 parts of alkaloid compound preparation (nicotine, caffeine, theobromine, matrine and colchicine in a mass ratio of 1:1.5:1:0.7:1.3), 2 parts of Dendrobium nobile stem extract, 3 parts of carnosic acid, 7 parts of composite fermentation liquid (earthworm fermentation liquid and chitin fermentation liquid in a mass ratio of 1:4), and 1 part of antimicrobial peptide.

[0077] Comparative Example 5

[0078] The difference from Example 1 is that the composite fermentation liquid is replaced with earthworm fermentation liquid, and the rest is the same as Example 1.

[0079] That is, the composite organic fertilizer of this comparative example includes the following raw materials in parts by weight: 45 parts of basic fertilizer, 8 parts of alkaloid compound preparation (nicotine, caffeine, theobromine, matrine and colchicine in a mass ratio of 1:1.5:1:0.7:1.3), 2 parts of Dendrobium nobile stem extract, 3 parts of carnosic acid, 7 parts of earthworm fermentation liquid, and 1 part of antimicrobial peptide.

[0080] Comparative Example 6

[0081] The difference from Example 1 is that the antimicrobial peptide is missing, and the rest is the same as Example 1.

[0082] Test example

[0083] 1. Banana wilt disease control effect

[0084] Variety: Brazilian banana

[0085] Experimental location: Banana planting base in Yulin City, Guangxi

[0086] Test method: The newly planted area (first generation seedlings) of the banana planting base is divided into 10 areas (control group and test group 1-9), each area is set with 3 treatments, each treatment has 20 rows, each row has 30 plants, 600 plants are planted, and the plant spacing is 2m×2m. The control group applies basic fertilizer, and the test group applies the composite organic fertilizer prepared by Examples 1-3 and Comparative Examples 1-6, and the application amount is 7 jin / plant, of which 4 jin / plant is used as base fertilizer, and 3 jin / plant is applied as topdressing after 3 months, and the field management of each treatment is consistent. The condition of banana wilt is investigated 1 month after topdressing, and the average banana yield per plant is counted after the bananas are ripe and picked, and the results are shown in Table 1.

[0087] Table 1

[0088]

[0089]

[0090] As can be seen from Table 1, compared with the control group, the organic compound fertilizer prepared in Examples 1-3 of the present invention has a better effect on preventing and treating banana wilt disease and a significant yield-increasing effect. Although Comparative Examples 1-6 are also better than the control group in preventing and treating banana wilt disease and increasing banana yield, the effect is far inferior to that of Examples 1-3.

[0091] 2. Effect of Phomopsis areca leaf spot disease control

[0092] Variety: Tobacco betel nut

[0093] Experimental location: Areca nut base in Yulin City, Guangxi

[0094] Test method: The planting area of ​​the betel nut base is divided into 9 areas (control group and test group 1-8), each area is set with 3 treatments, each treatment has 20 rows, 10 plants in each row, 200 plants are planted, and the plant spacing is 3m×3m. The control group applies basic fertilizer, and the test group applies the composite organic fertilizer prepared by Examples 1-3 and Comparative Examples 1-6, and the application amount is 10 jin / plant, of which 5 jin / plant is used as basal fertilizer, and 5 jin / plant is applied as topdressing after 5 months, and the field management of each treatment is consistent. The condition of the betel nut phomopsis leaf spot disease was investigated one month after the topdressing, and the results are shown in Table 2.

[0095] Table 2

[0096]

[0097]

[0098] As can be seen from Table 2, compared with the control group, the organic compound fertilizer prepared in Examples 1-3 of the present invention has a better effect in preventing and controlling Phomopsis areca leaf spot. Although Comparative Examples 1-6 are also better than the control group in preventing and controlling Phomopsis areca leaf spot, the effect is far inferior to that of Examples 1-3.

[0099] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A composite organic fertilizer, characterized in that: The raw materials include the following in parts by weight: 40-50 parts of basic fertilizer, 5-10 parts of alkaloid compound preparation, 1-3 parts of Dendrobium nobile stem extract, 2-4 parts of carnosic acid nutrient solution, 5-8 parts of compound fermentation liquid and 0.8-1.2 parts of antimicrobial peptide; The alkaloid compound preparation is nicotine, caffeine, theobromine, matrine and colchicine in a mass ratio of 1:1-2:0.8-1.2:0.5-0.8:1.2-1.5; The composite fermentation liquid is earthworm fermentation liquid and chitin fermentation liquid in a mass ratio of 1:3-5.

2. A composite organic fertilizer as claimed in claim 1, characterized in that, The preparation method of the carnosic acid nutrient solution comprises: adding carnosic acid to vegetable oil and mixing evenly to obtain an oil phase, adding amino acids to water and mixing evenly to obtain an aqueous phase, mixing the oil phase and the aqueous phase, adding polysorbate, and high-speed shearing to obtain the carnosic acid nutrient solution.

3. A composite organic fertilizer as claimed in claim 2, characterized in that, The solid-liquid ratio of carnosic acid to vegetable oil is 1:5-7 g / mL; the solid-liquid ratio of amino acids to water is 1:8-10 g / mL; the volume ratio of the oil phase to the water phase is 1:2-4; the mass ratio of the polysorbate to the oil phase is 0.1-0.3:1; and the high-speed shearing is performed at 2000-3000 rpm for 5-10 min.

4. A composite organic fertilizer as claimed in claim 1, characterized in that, The Dendrobium nobile stem extract contains 1.04-2.12 mg / g of alkaloids.

5. A composite organic fertilizer as claimed in claim 1, characterized in that, The antimicrobial peptides are antimicrobial peptides separated from the fermentation broth of Brevibacillus laterosporus and antimicrobial peptides separated from the fermentation broth of Trichoderma harzianum in a mass ratio of 1:1-1.

5.

6. A composite organic fertilizer as claimed in claim 1, characterized in that, The earthworm fermentation liquid is obtained by mixing and stirring chopped hungry earthworms and a composite enzyme; the mass ratio of the hungry earthworms to the composite enzyme is 100:0.1-0.5; the composite enzyme is papain and lipase in a mass ratio of 1:1-3; the mixing and stirring is performed at 100-200 rpm and 40-60° C. for 20-30 hours; The chitosan fermentation liquid is obtained by mixing and fermenting shrimp shells with composite bacterial liquid; the mass ratio of the shrimp shells to the composite bacterial liquid is 1:5-10; the effective colony count of the composite bacterial liquid is 10 6 -10 8 CFU / mL; the composite bacterial liquid is Lactobacillus bulgaricus and Streptococcus thermophilus in a mass ratio of 3-5:1; the fermentation temperature is 40-50°C and the fermentation time is 12-24h.

7. The method for preparing a composite organic fertilizer according to claim 1, wherein The specific steps include: (1) mixing the alkaloid composite preparation and the Dendrobium nobile stem extract uniformly, adding carnosic acid nutrient solution and composite fermentation liquid, and ultrasonically treating to obtain a mixed material; (2) The mixed material and the basic fertilizer are mixed, the pH is adjusted to be neutral, anaerobically fermented, dried and granulated to obtain the target compound organic fertilizer.

8. A method for preparing a composite organic fertilizer as claimed in claim 7, characterized in that, In step (1), the ultrasonic treatment is carried out at 20-40 kHz and 30-40° C. for 1-3 h; In step (2), the anaerobic fermentation is first performed at 50-60°C for 1-3 days, and then at 35-45°C for 2-4 days.

9. Use of the compound organic fertilizer according to any one of claims 1 to 6 in preventing and controlling plant diseases.

10. The use according to claim 9, characterized in that The plant diseases are Phomopsis areca leaf spot and Fusarium wilt of banana.

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