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Preparation of Streptomyces antibioticus and metabolite thereof and application of Streptomyces antibioticus in aspect of bacterium resistance

A technology against Streptomyces and metabolites, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and botanical equipment and methods, and can solve problems such as the growth of negative effects such as pesticide environmental pollution

Active Publication Date: 2020-07-14
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, chemical agents such as carbendazim and mancozeb are mainly used for prevention and control in production, but negative effects such as pesticide residues and environmental pollution are gradually increasing

Method used

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  • Preparation of Streptomyces antibioticus and metabolite thereof and application of Streptomyces antibioticus in aspect of bacterium resistance
  • Preparation of Streptomyces antibioticus and metabolite thereof and application of Streptomyces antibioticus in aspect of bacterium resistance
  • Preparation of Streptomyces antibioticus and metabolite thereof and application of Streptomyces antibioticus in aspect of bacterium resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: Isolation and cultivation of marine actinomycetes

[0045] 1. Biological material and culture medium: The biological material used to isolate marine actinomycetes was collected from mangroves in Enping City, Guangdong Province, with a total of 792 samples. The medium used for the isolation of actinomycetes is Gao's No. 1 medium (in order to ensure the quantity and type of isolated actinomycetes, the separation medium is prepared with artificial seawater, and the formula is: 20 g of soluble corn starch, KNO 3 1g, K 2 HPO4 0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 ·7H 2 O 0.01g, NaCl 0.5g, agar 20g, artificial seawater 1000mL, pH 7.2~7.4; the artificial seawater formula is: NaCl 24.477g, MgCl 2 ·6H 2 O 4.981g, Na 2 SO 4 3.917g, CaCl 2 2H 2 O 1.102g, KCl 0.664g, NaHCO 3 0.192g, KBr 0.096g, H 3 BO 3 0.026g, SrCl 2 0.024g, NaF0.0039g, distilled water 1000mL). The media used in the routine culture of actinomycetes in the following experiments were pr...

Embodiment 2

[0048] Example 2: Screening, isolation and identification of antagonistic marine actinomycetes

[0049] 1. Screening and isolation of antagonistic marine actinomycetes

[0050] 1.1 Test strains and culture medium: use Candida albicans (Candida albican) (purchased in Microbiology Research of Guangdong Province) as indicator bacteria, measure the inhibitory activity of 792 isolated actinomycetes to Candida albicans, to screen out the inhibitory activity against Candida albicans therefrom The strain with the strongest inhibitory activity against Candida. The culture medium used for actinomycetes cultivation is Gao Shi No. 1 medium (specific formula as described in embodiment 1); , yeast extract 4g, K 2 HPO 4 ·7H 2 O 0.63g, MgSO 4 ·7H 2 O 1.8g, agar 15g, add water to make up to 1000mL).

[0051] 1.2 Activation of bacterial strains: prepare the corresponding medium according to the above formula, heat and dissolve the medium in the conical flask and pour it into a 9 cm diame...

Embodiment 3

[0085] The preparation of embodiment 3 metabolites

[0086] The extract of the present invention can be obtained by extracting and separating from the ethyl acetate extract of the culture (fermentation liquid) of marine actinomycetes PPI-16, and its specific preparation method comprises the following steps:

[0087] (1) Seed culture of marine actinomycetes PPI-16:

[0088] Pick the strains and insert them into the slant medium, and cultivate them at 28-30°C for 3-5 days; among them,

[0089] Medium (by weight): 5g soluble corn starch, 10g glucose, 3g yeast extract, 2g peptone, KNO 3 1g, K 2 HPO 4 0.5g, NaCl 0.5g, FeSO 4 0.01g, MgSO 4 ·7H 2 O 0.05g, agar 15g, pH7.4, dilute to 1000mL with water; make a test tube slope.

[0090] (2) Fermentation and cultivation of marine actinomycetes PPI-16:

[0091] Pick the cultured strain (inoculum size is 5%) in the slant into the fermentation medium, and ferment and cultivate it at room temperature 28°C for 5 days; wherein, the f...

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Abstract

The invention discloses preparation of Streptomyces antibioticus and metabolite thereof and application of the Streptomyces antibioticus in the aspect of bacterium resistance. The strain is named as Streptomyces antibioticus PPI-16, the preservation number is GDMCC NO: 60970, and the strain was preserved in the Guangdong Microbiological Culture Collection Center in the fifth floor, No. 59 Building, No. 100 Courtyard, Xianliezhong road, Guangzhou City on March 5, 2020. Active compounds generated by the Streptomyces antibioticus can inhibit various pathogenic fungi such as Colletotrichum lagenarium, Colletotrichum acutatum, Colletotrichum nicotianae and Candida albicans, and new resources are provided for research and development of antibiotic.

Description

technical field [0001] The invention belongs to the field of biopesticide research, in particular to the preparation of a strain of antibiotic streptomyces and its metabolite and its application in antibacterial aspects. Background technique [0002] With the widespread application of broad-spectrum antibiotics and immunosuppressants and the development of invasive treatments, new mycoses are emerging, and the incidence of some ancient mycoses has begun to rise, especially the increasing number of opportunistic fungal infections. The increasing incidence of fungal infections (especially invasive fungal infections) has caused serious harm to human health. Human fungal infections can be divided into 3 types: superficial mycoses, deep mycoses, and systemic mycoses (Garber, 2001), among which the most serious and life-threatening fungal infections are systemic mycoses (Meis, 2001) . The pathogenic bacteria of deep mycoses are mainly Candida, especially Candida albicans. It is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N3/00C12P17/08A01N63/28A01P3/00C12R1/48
CPCC12N1/20C12N3/00C12P17/08A01N63/00C12R2001/48C12N1/205A01N63/28A01P3/00
Inventor 杨祁云林壁润路征张景欣孙大元吕永华沈会芳蒲小明
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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