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Microbial deodorizing microbial inoculum as well as preparation method and application thereof

A deodorant bacterium and microbial technology, applied in the field of microbial deodorant bacterium and its preparation, can solve the problems of weak environmental adaptability, metabolism type, respiration type and single function, etc., achieve strong environmental adaptability and improve ecology The environment, the effect of inhibiting growth and reproduction

Inactive Publication Date: 2014-01-29
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Odor release sources such as public toilets, livestock and poultry farms, manure storage tanks, and garbage collection stations are micro-ecosystems with complex nutrients and diverse indigenous microbial communities. Whether it is the key to achieve odor control, because of its single metabolic type, respiratory type, and single function, it is difficult for a single strain to become the dominant bacterial group in the odor release source because of its weak adaptability to the environment

Method used

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  • Microbial deodorizing microbial inoculum as well as preparation method and application thereof
  • Microbial deodorizing microbial inoculum as well as preparation method and application thereof
  • Microbial deodorizing microbial inoculum as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) Quantify YPD, MRS, beef extract peptone and Gaoshi No. 1 solid medium after high-temperature sterilization (121°C, 30 minutes) for standby use. The formula of solid YPD medium is 20g of peptone, 10g of beef extract, and 20g of glucose , water 1000mL, agar 15-20g, pH6.5-7.0; solid MRS medium formula is peptone 10g, beef extract 10g, yeast extract 5g, glucose 5g, sodium acetate 5g, diammonium citrate 2g, Tween 801mL, sulfuric acid Magnesium 0.58g, manganese sulfate 0.05g, dipotassium hydrogen phosphate 2g, agar 15-20g, water 1000mL, pH6.2; beef extract peptone solid medium is beef extract 5g, peptone 10g, sodium chloride 5g, agar 15-20g , water 1000mL, pH7.0-7.5; Gaoshi No. 1 solid medium is starch 20g, potassium nitrate 1g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, sodium chloride 0.5g, ferrous sulfate heptahydrate 0.01g , Agar 15-20g, water 1000mL, pH7.2-7.4.

[0050] (2) Insert the CR and SX strains into 1 ring respectively and activate them in ...

Embodiment 2

[0058] The same as Example 1 will not be repeated, the difference is that the 6 bacterial solutions are mixed in the ratio of CR:SX:NG:SR:DY:XH=1:1:2:2:1.5:1.5 (volume / volume) .

[0059] The indicators of the prepared liquid microbial deodorant agent: the number of viable bacteria is equal to 1.1 billion CFU / mL; pH5; suspension, yellowish in color, slightly sour.

[0060] The indicators of the prepared solid microbial deodorant agent: the number of viable bacteria is equal to 1.1 billion CFU / g; the particles are dry, the surface is milky white, and there is a slight sour taste.

Embodiment 3

[0062] The same as Example 1 will not be repeated, the difference is that the 6 bacterial solutions are mixed according to the ratio of CR:SX:NG:SR:DY:XH=2:2:1.5:1.5:1:1 (volume / volume) .

[0063] The indicators of the prepared liquid microbial deodorant agent: the number of viable bacteria is equal to 1 billion CFU / mL; pH6; suspension, slightly yellowish in color, with wine aroma.

[0064] The indicators of the prepared solid microbial deodorant agent: the number of viable bacteria is equal to 1 billion CFU / g; the particles are dry, the surface is milky white, and there is wine fragrance.

[0065] 2. Application of microbial deodorizer

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Abstract

The invention discloses a microbial deodorizing microbial inoculum for public toilets, livestock and poultry farms and refuse collection points. The microbial deodorizing microbial inoculum contains pichia jadinii with the preservation number of CGMCCNO.5982, issatchenkia orientalis with the preservation number of CGMCCNO.6496, lactobacillus sp. with the preservation number of CGMCCNO.6495, streptococcus thermophilus with the preservation number of CGMCCNO.5985, bacillus licheniformis with the preservation number of CICCNO.20085 and streptomyces microflavus var. lactosus with the preservation number of CGMCCNO.4891. The invention also discloses a preparation method of the microbial deodorizing microbial inoculum in both liquid and solid states, and application of the microbial deodorizing microbial inoculum.

Description

technical field [0001] The invention relates to a microbial deodorizing agent for public toilets, livestock and poultry farms and garbage collection stations and a preparation method thereof, in particular to utilizing the symbiosis, intergrowth, synergistic utilization and metabolism of bacterial strains according to the principles of microbial ecology A microbial deodorizing agent with various types, respiration types and functions, its preparation method and its application. Background technique [0002] With the rapid development of my country's economy, people have gradually changed from material life to spiritual life, tourism has developed rapidly, and the number of public toilets in scenic spots has continued to increase. The control of toilet odor in scenic spots and the disposal of feces have become important factors affecting the sustainable development of scenic spots. At the same time, as of 2012, the output value of my country's livestock and poultry breeding i...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/18A61L9/01C12R1/84C12R1/225C12R1/46C12R1/10C12R1/465
Inventor 闫志英刘晓风袁月祥许力山李东
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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