Method for inducing Colletotrichum gloesporioides to generate conidia
A technology of conidia and anthrax bacteria, applied in the direction of using spores, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problem of no effective method for inducing spore production of anthrax bacteria, and achieve a large amount of spore production and Stable, huge spore production, convenient and fast drying effect
Active Publication Date: 2018-12-14
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology
[0006] Aiming at the problem that the existing anthrax bacteria has no effective method for inducing the production of spores, the present invention provides a method for inducing the production of conidia in the anthrax bacteria, which can effectively induce the mass production of anthrax spores
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Embodiment 1
[0044] A method for inducing anthrax bacteria to produce conidia, comprising the steps of:
[0045] (1) Activation of G. anthracnose: On a clean bench, inoculate PDA plate culture medium with G. anthracis (strain purchased from China Agricultural Microorganism Culture Collection Management Center, strain number: ACCC 31200) preserved at 4°C The activated culture is carried out on the above-mentioned activated culture, and the condition of the described activated culture is to cultivate in the dark at a temperature of 27° C. for 5 days to obtain the activated G. anthracnose;
[0046] (2) Naturally induce spore production (natural sporulation method): get the activated glyosporium anthracis in step (1) and put it into a constant temperature incubator for constant temperature cultivation. The specific steps are as follows: on the ultra-clean workbench, use a diameter of A 0.5cm hole puncher is punched at the colony edge of the PDA plate culture medium, and the mycelia block (i.e....
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Abstract
The invention discloses a method for inducing Colletotrichum gloesporioides to generate conidia, belonging to the technical field of plant protection. The method comprises the following steps: (1) activation of Colletotrichum gloesporioides: inoculating a culture medium with the Colletotrichum gloesporioides, and carrying out activation culturing so as to obtain activated Colletotrichum gloesporioides; and (2) generation of the conidia by induction: putting the Colletotrichum gloesporioides activated in the step (1) into an oven, carrying out high-temperature culturing, then taking cultured Colletotrichum gloesporioides out of the oven, placing the cultured Colletotrichum gloesporioides into a constant-temperature incubator with a temperature of 27 DEG C, and carrying out constant-temperature culturing for 7 days so as to realize induction of the generation the conidia. According to the invention, the Colletotrichum gloesporioides is successively subjected to high-temperature culturingand constant-temperature culturing for the first time, so the method provided by the invention realizes simple, convenient and large-scale induction of Colletotrichum gloesporioides for generation ofa large number of conidia, and has important significance to researches on determination of pathogenic ability of the Colletotrichum gloesporioides, determination of biological activity of a bactericide of the Colletotrichum gloesporioides, identification of disease resistance of a variety, etc.
Description
technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a method for inducing anthracnose bacteria to produce conidia. Background technique [0002] Crop anthracnose is a fungal disease caused by anthracnose bacteria (Colletotrichum sp.). In addition to infecting field crops, it can also damage various fruit trees and flowers such as figs, peaches, pears, grapes and citrus. With climate change, the disease is becoming more and more serious, becoming the main disease affecting crop yield and quality. In order to be used in studies such as the determination of the pathogenicity of anthrax bacteria, the determination of the biological activity of fungicides, and the identification of disease resistance of varieties, the conventional methods for inducing spore production of anthrax bacteria are the natural sporulation method and the yeast PDA induction method: [0003] Natural sporulation method: pick mycelium preser...
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IPC IPC(8): C12N3/00C12N1/14C12R1/645
CPCC12N1/14C12N3/00
Inventor 檀根甲李丹丹汪章勋叶磊刘灿
Owner ANHUI AGRICULTURAL UNIVERSITY
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